A novel subtilisin-like serine protease from Thermoanaerobacter yonseiensis KB-1: Its cloning, expression, and biochemical properties

Extremophiles

Volumn 6, Issue 3, 2002, Pages 233-243

  Hyeung, Jin Jang ^a   Byoung, Chan Kim ^a   Yu, Ryang Pyun ^a   Yu, Sam Kim ^a  

^a Yonsei University   (South Korea)

Author keywords

Phylogenetic analysis; Subtilisin like serine protease; Thermicin; Thermoanaerobacter yonseiensis KB 1

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THERMOANAEROBACTER YONSEIENSIS;

BACTERIAL DNA; COLLAGEN; FIBRONECTIN; SERINE PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; GRAM POSITIVE BACTERIUM; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; COLLAGEN; DNA, BACTERIAL; FIBRONECTINS; GRAM-POSITIVE BACTERIA; MOLECULAR SEQUENCE DATA; PHYLOGENY; SEQUENCE HOMOLOGY, AMINO ACID; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY;

EID: 0036594250     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-001-0248-1     Document Type: Article

References (33)

1. Abraham LD, Chow DT, Breuil C (1995) Characterization of the cleavage specificity of a subtilisin-like proteinase from Ophiostoma piceae by liquid chromatography/mass spectrometry and tandem MS. FEBS Lett 374:208-210
2. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402
3. Basak A, Toure BB, Lazure C, Mbikay M, Chretien M, Seidah NG (1999) Enzymic characterization in vitro of recombinant proprotein convertase PC4. Biochem J 343:29-37
4. Blumentals II, Robinson AS, Kelly RM (1990) Characterization of sodium dodecyl sulfate-resistant proteolytic activity in the hyperthermophilic archaebacterium Pyrococcus furiosus. Appl Environ Microbiol 56:1992-1998
5. Chavez Croocker P, Sako Y, Uchida A (1999) Purification and characterization of an intracellular heat-stable proteinase (pernilase) from the marine hyperthermophilic archaeon Aeropyrum pernix K1. Extremophiles 3:3-9
6. Choi IG, Bang WG, Kim SH, Yu YG (1999) Extremely thermostable serine-type protease from Aquifex pyrophilus. Molecular cloning, expression, and characterization. J Biol Chem 274:881-888
7. Cowan DA, Smolenski KA, Daniel RM, Morgan HW (1987) An extremely thermostable extracellular proteinase from a strain of the archaebacterium Desulfurococcus growing at 88°C. Biochem J 247:121-133
8. Harrington DJ (1996) Bacterial collagenases and collagen-degrading enzymes and their potential role in human disease. Infect Immun 64:1885-1891
9. Heussen C, Dowdle EB (1980) Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Anal Biochem 102:196-202
10. Ikemura H, Inouye M (1988) In vitro processing of pro-subtilisin produced in Escherichia coli. J Biol Chem 263:12959-12963
11. Jeon OH, Kim DS (1999) Molecular cloning and functional characterization of a snake venom metalloprotease. Eur J Biochem 263:526-533
12. Kelleher PJ, Juliano RL (1984) Detection of proteases in polyacrylamide gels containing covalently bound substrates. Anal Biochem 136:470-475
13. Kim BC, Grote R, Lee DW, Antranikian G, Pyun YR (2001) Thermoanaerobacter yonseiensis sp. nov., a novel extreme thermophilic, xylose-utilizing bacterium that grows at up to 85°C. Int J Syst Evol Microbiol 51:1539-1548
14. Klingeberg M, Hashwa F, Antranikian G (1991) Properties of extremely thermostable proteases from anaerobic hyperthermophilic bacteria. Appl Microbiol Biotechnol 34:715-719
15. Klingeberg M, Galunsky B, Sjoholm C, Kasche V, Antranikian G (1995) Purification and properties of a highly thermostable, sodium dodecyl sulfate-resistant and stereospecific proteinase from the extremely thermophilic archaeon Thermococcus stetteri. Appl Environ Microbiol 61:3098-3104
16. Koch R, Zablowsky R Spreinat A, Antranikian G (1990) Extremely thermostable amylolytic enzyme from the archaebacterium Pyrococcus furiosus. FEMS Microbiol Lett 71:21-26
17. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
18. Li Y, Inouye M (1996) The mechanism of autoprocessing of the propeptide of prosubtilisin E: intramolecular or intermolecular event? J Mol Biol 262:591-594
19. Morikawa M, Izawa Y, Rashid N, Hoaki T, Imanaka T (1994) Purification and characterization of a thermostable thiol protease from a newly isolated hyperthermophilic Pyrococcus sp. Appl Environ Microbiol 60:4559-4566
20. Nagase H, Fields CG, Fields GB (1994) Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin I (matrix metalloproteinase-3). J Biol Chem 269:20952-20957
21. Ng T K, Kenealy WR (1986) Industrial application of thermostable enzymes. In: Brock TD (ed) Thermophiles, general, molecular, and applied microbiology. Wiley, New York, pp 197-215
22. Page RDM (1996) Tree View: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357-358
23. Peek K, Daniel RM, Monk C, Parker L, Coolbear T (1992) Purification and characterization of a thermostable proteinase isolated from Thermus sp. strain Rt41A. Eur J Biochem 207:1035-1044
24. Peek K, Veitch DR Prescott M, Daniel RM, MacIver B, Bergquist PL (1993) Some characteristics of a proteinase from a thermophilic Bacillus sp. expressed in Escherichia coli: comparison with the native enzyme and its processing in E. coli and in vitro. Appl Environ Microbiol 59:1168-1175
25. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597-635
26. Saeki K, Okuda M, Hatada Y, Kobayashi T, Ito S, Takami H, Horikoshi K (2000) Novel oxidatively stable subtilisin-like serine proteases from alkaliphilic Bacillus spp.: enzymatic properties, sequences, and evolutionary relationships. Biochem Biophys Res Commun 279:313-319
27. Saitou N, Nei M (1987) A neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 44: 406-425
28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Laboratory Press, Cold Spring Harbor, NY, USA
29. Siezen RJ, Leunissen JA (1997) Subtilases: the superfamily of subtilisin-like serine proteases. Protein Sci 6:501-523
30. Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignments through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680
31. Völkl P, Markiewicz P, Stetter KO, Miller JH (1994) The sequence of a subtilisin-type protease (aerolysin) from the hyperthermophilic archaeum Pyrobaculum aerophilum reveals sites important to thermostability. Protein Sci 3:1329-1340
32. Wells JA, Powers DB, Bott RR, Graycar TP, Estel DA (1987) Designing substrate specificity by protein engineering of electrostatic interactions. Proc Natl Acad Sci U S A 84:1219-1223
33. Yip KS, Stillman TJ, Britton KL, Artymiuk PJ, Baker PJ, Sedelnikova SE, Engel PC, Pasquo A, Chiaraluce R, Consalvi V (1995) The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures. Structure 3:1147-1158