Exercise with low muscle glycogen augments TCA cycle anaplerosis but impairs oxidative energy provision in humans

Journal of Physiology

Volumn 540, Issue 3, 2002, Pages 1079-1086

  Gibala, Martin J ^a,c   Peirce, Nick ^b   Constantin Teodosiu, Dimitru ^a   Greenhaff, Paul L ^a  

^a Rigshospitalet   (Denmark)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^c MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCARNITINE; ALANINE AMINOTRANSFERASE; CREATINE PHOSPHATE; GLYCOGEN; OXYGEN; PYRUVATE DEHYDROGENASE; PYRUVIC ACID; TRICARBOXYLIC ACID; ALANINE; CARNITINE; GLUTAMIC ACID; LACTIC ACID; PYRUVATE DEHYDROGENASE COMPLEX;

ADULT; ARTICLE; ATTENUATION; CARBOHYDRATE INTAKE; CITRIC ACID CYCLE; CONTROLLED STUDY; CYCLING; DIET RESTRICTION; DIETARY INTAKE; ENERGY RESOURCE; ENZYME ACTIVITY; EXERCISE; EXHAUSTION; GLYCOGEN MUSCLE LEVEL; GLYCOGENOLYSIS; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; MALE; MUSCLE BIOPSY; MUSCLE CONTRACTION; MUSCLE RELAXATION; NORMAL HUMAN; OXIDATION; OXYGEN CONSUMPTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; REST; TIME; TRANSPORT KINETICS; VASTUS LATERALIS MUSCLE; AEROBIC METABOLISM; ANAPLEROSIS; LOW CARBOHYDRATE DIET; MUSCLE METABOLISM;

ADULT; ALANINE; ALANINE TRANSAMINASE; CITRIC ACID CYCLE; ENERGY METABOLISM; EXERCISE; EXERCISE TEST; GLUTAMIC ACID; GLYCOGEN; HUMANS; KINETICS; LUNG; MALE; MUSCLE, SKELETAL; OXYGEN CONSUMPTION; PYRUVATE DEHYDROGENASE COMPLEX; RESPIRATORY MECHANICS; REST; TRICARBOXYLIC ACIDS;

EID: 0036588469     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1113/jphysiol.2001.012983     Document Type: Article

References (33)

1. BERGMEYER, H. U. (1974). Methods of Enzymatic Analysis, 2nd edn. Academic Press, New York.
2. BERGMEYER, H. U., M. GRAßL, M. & WALTER H.-E. (1983). Section I: Enzymes. In Methods of Enzymatic Analysis, 3rd edn., ed. BERGMEYER, H. U., pp. 136-138. Verlag Chemie, Weinheim, Germany.
3. BERGSTRÖM, J., FÜRST, P. & HULTMAN, E. (1985). Free amino acids in muscle tissue and plasma during exercise in man. Clinical Physiology 5, 155-160.
4. BRUCE, M., CONSTANTIN-TEODOSIU, D., GREENHAFF, P. L., BOOBIS, L. H., WILLIAMS, C. & BOWTELL, J. L. (2001). Glutamine supplementation promotes anaplerosis but not oxidative energy delivery in human skeletal muscle. American Journal of Physiology - Endocrinology and Metabolism 280, E669-675.
5. CEDERBLAD, G., CARLIN, J. L, CONSTANTIN-TEODOSIU, D., HARPER, P. & HULTMAN, E. (1990). Radioisotopic assays of CoASH and carnitine and their acetylated forms in human skeletal muscle. Analytical Biochemistry 185, 274-278.
6. CHRISTIE, A. & BUTLER, M. (1999). The adaptation of BHK cells to a non-ammoniagenic glutamate-based culture medium. Biotechnology and Bioengineering 64, 298-309.
7. CONSTANTIN-TEODOSIU, D., CARLIN, J. L, CEDERBLAD, G., HARRIS, R. C. & HULTMAN, E. (1991a). Acetyl group accumulation and pyruvate dehydrogenase activity in human muscle during incremental exercise. Acta Physiologica Scandinavica 143, 367-372.
8. CONSTANTIN-TEODOSIU, D., CEDERBLAD, G. & HULTMAN, E. (1991b). A sensitive radioisotopic assay of pyruvate dehydrogenase complex in human muscle tissue. Analytical Biochemistry 198, 347-351.
9. CONSTANTIN-TEODOSIU, D. & GREENHAFF, P. L. (1999). The tricarboxylic acid cycle in human skeletal muscle: Is there a role for nutritional intervention? Current Opinions in Clinical Nutrition and Metabolic Care 2, 527-531.
10. CONSTANTIN-TEODOSIU, D., SIMPSON, E. J. & GREENHAFF, P. L. (1999). The importance of pyruvate availability to PDC activation and anaplerosis in human skeletal muscle. American Journal of Physiology 276, E472-478.
11. DYCK, D. J., PUTMAN, C. T., HEIGENHAUSER, G. J. F., HULTMAN, E. & SPRIET, L. L. (1993). Regulation of fat-carbohydrate interaction in skeletal muscle during intense aerobic cycling. American Journal of Physiology 265, E852-859.
12. GIBALA, M. J., LOZEJ, M., TARNOPOLSKY, M. A., MCLEAN, C. & GRAHAM, T. E. (1999). Low glycogen and branched-chain amino acid ingestion do not impair anaplerosis during exercise in humans. Journal of Applied Physiology 87, 1662-1667.
13. GIBALA, M. J., MACLEAN, D. A., GRAHAM, T. E. & SALTIN, B. (1997). Anaplerotic processes in human skeletal muscle during brief dynamic exercise. Journal of Physiology 502, 703-713.
14. GIBALA, M. J., MACLEAN, D. A., GRAHAM, T. E. & SALTIN, B. (1998). Tricarboxylic acid cycle pool size and estimated cycle flux in human muscle during exercise. American Journal of Physiology 275, E235-242.
15. GIBALA, M. J. & SALTIN, B. (1999). PDH activation by dichloroacetate reduces TCA cycle intermediates at rest but not during exercise in humans. American Journal of Physiology 277, E33-38.
16. GRAHAM, T. E. & GIBALA, M. J. (1998). Anaplerosis of the tricarboxylic acid cycle during exercise in human skeletal muscle: Magnitude, sources and potential physiological significance. In Skeletal Muscle Metabolism in Exercise and Diabetes, ed. RICHTER, E. A., GALBO, H., KIENS, B. & SALTIN, B., pp. 271-286. Plenum Press, New York.
17. GREENHAFF, P. L., GLEESON, M. & MAUGHAN, R. J. (1988). The effects of diet on muscle pH and metabolism during high intensity exercise. European Journal of Applied Physiology 57, 531-539.
18. HARRIS, R. C., HULTMAN, E. & NORDESJO, L. O. (1974). Glycogen, glycolytic intermediates and high-energy phosphates determined in biopsy samples of musculus quadriceps femoris of man at rest. Scandinavian Journal of Clinical and Laboratory Investigation 33, 109-120.
19. HULTMAN, E., BERGSTRÖM, J. & MCLEANNAN ANDERSON, N. (1967). Breakdown and resynthesis of phosphocreatine and adenosine triphosphate in connection with muscular work in man. Scandinavian Journal of Clinical and Laboratory Investigation 19, 56-66.
20. PARDRIDGE, W. M. & DAVIDSON, M. B. (1979). Alanine metabolism in skeletal muscle in tissue culture. Biochimica Biophysica Acta 585, 34-42.
21. PASSONEAU, J. V. & LOWRY, O. H. (1993). Enzymatic Analysis: A Practical Guide. Humana Press, Totowa, NJ, USA.
22. PETERS, S. J., ST AMAND, T. A., HOWLETT, R. A., HEIGENHAUSER, G. J. F. & SPRIET, L. L. (1998). Human skeletal muscle pyruvate dehydrogenase kinase activity increases after a low-carbohydrate diet. American Journal of Physiology 275, E980-986.
23. PUTMAN, C. L., SPRIET, L. L., HULTMAN, E., LINDINGER, M. I., LANDS, L. C., MCKELVIE, R. S., CEDERBLAD, G., JONES, N. L. & HEIGENHAUSER, G. J. F. (1993). Pyruvate dehydrogenase activity and acetyl group accumulation during exercise after different diets. American Journal of Physiology 265, E752-760.
24. RANDLE, P.J. (1995). Metabolic fuel selection: general integration at the whole-body level. Proceedings of the Nutrition Society 54, 317-327.
25. REN, J. M., BROBERG, S., SAHLIN, K. & HULTMAN, E. (1990). Influence of reduced glycogen level on glycogenolysis during short-term stimulation in man. Acta Physiologica Scandinavica 139, 467-474.
26. SAHLIN, K., KATZ, A. & BROBERG, S. (1990). Tricarboxylic acid cycle intermediates in human muscle during prolonged exercise. American Journal of Physiology 259, C834-841.
27. SNELL, K. (1980). Muscle alanine synthesis and hepatic gluconeogenesis. Biochemical Society Transactions 8, 205-213.
28. SPENCER, M. K., ZHEN, Y. & KATZ, A. (1992). Effect of low glycogen on carbohydrate and energy metabolism in human muscle during exercise. American Journal of Physiology 262, C975-979.
29. TSCHAKOVKSY, M. E. & HUGHSON, R. L. (1999). Interaction of factors determining oxygen uptake at the onset of exercise. Journal of Applied Physiology 86, 1101-1113.
30. TIMMONS, J. A., GUSTAFSSON, T., SUNDBERG, C. J., JANSSON, E. & GREENHAFF, P. L. (1998). Muscle acetyl group availability is a major determinant of oxygen deficit in humans during submaximal exercise. American Journal of Physiology 274, E377-380.
31. TIMMONS, J. A., POUCHER, S. M., CONSTANTIN-TEODOSIU, D., MACDONALD, I. & GREENHAFF, P. L. (1997). The metabolic responses from rest to steady-state determine contractile function in ischemic canine skeletal muscle. American Journal of Physiology 273, E233-238.
32. VAN HALL, G., SALTIN, B., VAN DER VUSSE, G. J., SÖDERLAND, K. & WAGENMAKERS, A. J. M. (1995). Deamination of amino acids as a source for ammonia production in human skeletal muscle during prolonged exercise. Journal of Physiology 489, 251-261.
33. WAGENMAKERS, A. J. M. (1998). Muscle amino acid metabolism at rest and during exercise: Role in human physiology and metabolism. In Exercise and Sports Science Reviews, ed. HOLLOSZY, J. O., pp. 287-314. Williams and Wilkins, Baltimore, MD, USA.