Efficient in vitro transduction of epithelial cells and keratinocytes with improved adenoviral gene transfer for the application in skin tissue engineering

Transplant Immunology

Volumn 9, Issue 2-4, 2002, Pages 323-329

  Doebis, Cornelia ^a   Ritter, Thomas ^a   Brandt, Christine ^a   Schönberger, Bernd ^a   Volk, Hans Dieter ^a   Seifert, Martina ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

Gene therapy; Keratinocytes; Polycation; Skin transplantation

Indexed keywords

ADENOVIRUS VECTOR; BETA GALACTOSIDASE; HEXADIMETHRINE BROMIDE; POLYCATION;

ANIMAL CELL; ASSAY; CELL COUNT; CONFERENCE PAPER; CONTROLLED STUDY; DISEASE COURSE; DOSE RESPONSE; DRUG EFFECT; DRUG EFFICACY; DRUG EXPOSURE; EPITHELIUM CELL; ESCHERICHIA COLI; GENE FUNCTION; GENE TRANSFER; GENETIC REGULATION; GENETIC TRANSDUCTION; HOST RESISTANCE; HUMAN; HUMAN CELL; IN VITRO STUDY; INFECTION; INFLAMMATION; KERATINOCYTE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REPORTER GENE; SIGNAL TRANSDUCTION; SKIN TRANSPLANTATION; STAINING; TISSUE ENGINEERING; VIRAL GENE THERAPY; VIRUS GENE; VIRUS PARTICLE; VIRUS RECOMBINANT; ADENOVIRUS; ANIMAL; ARTICLE; CELL LINE; GENE THERAPY; GENETICS; METABOLISM; METHODOLOGY;

ADENOVIRIDAE; ANIMALS; CELL LINE; EPITHELIAL CELLS; GENE THERAPY; GENE TRANSFER TECHNIQUES; HEXADIMETHRINE; HUMANS; KERATINOCYTES; RATS; SKIN TRANSPLANTATION; TISSUE ENGINEERING;

EID: 0036580019     PISSN: 09663274     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0966-3274(02)00034-5     Document Type: Conference Paper

References (19)

1. Gene therapy - Promises, problems and prospects
2. Adenovirus-mediated gene transfer for cystic fibrosis: Part A. Safety of dose and repeat administration in the nasal epithelium. Part B. Clinical efficacy in the maxillary sinus
3. Gene therapy for lung cancer and mesothelioma
4. Adenovirus-mediated transfer of p33ING1 with p53 drastically augments apoptosis in gliomas
5. Ex vivo and in vivo gene transfer to the skin using replication-deficient recombinant adenovirus vectors
6. Inefficient gene transfer by adenovirus vector to cystic fibrosis airway epithelia of mice and humans
7. Polycations increase the efficiency of adenovirus-mediated gene transfer to epithelial and endothelial cells in vitro
8. Cellular and humoral immune responses to viral antigens create barriers to lung-directed gene therapy with recombinant adenoviruses
9. Gene therapy
10. CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts
11. Variability of adenovirus receptor density influences gene transfer efficiency and therapeutic response in head and neck cancer
12. Use of protamine to augment adenovirus-mediated cancer gene therapy
13. Polycations and cationic lipids enhance adenovirus transduction and transgene expression in tumor cells
14. Cationic lipids and polymers are able to enhance adenoviral infection of cultured mouse myotubes
15. Prolonged and effective blockade of tumor necrosis factor activity through adenovirus-mediated gene transfer
16. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte line
17. Cell receptors involved in adenovirus entry
18. Retroviral vector-mediated gene transfer into keratocytes: In vitro effects of polybrene and protamine sulfate
19. Potentiation of gene transfer to the mouse lung by complexes of adenovirus vector and polycations improves therapeutic potential