Post-termination complex disassembly by ribosome recycling factor, a functional tRNA mimic

EMBO Journal

Volumn 21, Issue 9, 2002, Pages 2272-2281

  Hirokawa, Go ^a   Kiel, Michael C ^a   Muto, Aiko ^a   Selmer, Maria ^b   Raj, V Samuel ^c   Liljas, Anders ^d   Igarashi, Kazuei ^e   Kaji, Hideko ^e   Kaji, Akira ^f  

^a DIVISION OF REPRODUCTIVE BIOLOGY   (United States)

^b UNIVERSITY OF LONDON   (United Kingdom)

^c RESEARCH AND DEVELOPMENT   (Australia)

^d College of Chemistry and Molecular Engineering ^*  (China)

^e UNIVERSITY OF SHEFFIELD   (United Kingdom)

^f UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Antibiotics; Elongation factor G; Protein synthesis; RRF; Translocation

Indexed keywords

AMINOGLYCOSIDE ANTIBIOTIC AGENT; ANTIBIOTIC AGENT; ANTIBIOTIC G 418; ELONGATION FACTOR G; FUSIDIC ACID; GENTAMICIN; KANAMYCIN; NEOMYCIN; PAROMOMYCIN; PROTEIN; RIBOSOME RECYCLING FACTOR; STREPTOMYCIN; THIOSTREPTON; TOBRAMYCIN; TRANSFER RNA; UNCLASSIFIED DRUG; VIOMYCIN;

ARTICLE; DRUG EFFECT; MODEL; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; RIBOSOME; RNA ANALYSIS; RNA TRANSPORT;

ESCHERICHIA COLI; MACROMOLECULAR SUBSTANCES; PEPTIDE CHAIN TERMINATION, TRANSLATIONAL; PEPTIDE ELONGATION FACTOR G; PROTEIN SYNTHESIS INHIBITORS; PROTEINS; RIBOSOMAL PROTEINS; RIBOSOMES; RNA, MESSENGER; RNA, TRANSFER;

EID: 0036566330     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/21.9.2272     Document Type: Article

References (65)

1. Visualization of elongation factor G on the Escherichia coli 70S ribosome: The mechanism of translocation
2. Studies of elongation factor G-dependent tRNA translocation by three-dimensional cryo-electron microscopy
3. Formation of the ribosome-G factor-GDP complex in the presence of fusidic acid
4. Studies on translocation. VI. Thiostrepton prevents the formation of a ribosome-G factor-guanine nucleotide complex
5. Inhibition of ribosomal translocation by aminoglycoside antibiotics
6. Recognition sites for antibiotics within rRNA
7. Streptomycin, suppression and the code
8. A ratchet-like inter-subunit reorganization of the ribosome during translocation
9. Cryo-electron microscopy of the translational apparatus: Experimental evidence for the paths of mRNA, tRNA and the polypeptide chain
10. Non-enzymatic translation
11. The allosteric three-site model for the ribosomal elongation cycle. New insights into the inhibition mechanisms of aminoglycosides, thiostrepton and viomycin
12. Factor-dependent release of ribosomes from messenger RNA. Requirement for two heat-stable factors
13. Role of elongation factor G and a protein factor on the release of ribosomes from messenger ribonucleic acid
14. Mechanism of translocation: Effect of cognate transfer ribonucleic acids on the binding of AUGUn to 70S ribosomes
15. Comparative studies on the mechanism of action of lincomycin, streptomycin and erythromycin
16. Role of ribosome recycling factor (RRF) in translational coupling
17. The role of guanosine triphosphate in translocation reaction catalyzed by elongation factor G
18. Interaction of ribosome recycling factor and elongation factor EF-G with E. coli ribosomes studied by the surface plasmon resonance technique
19. Release of transfer ribonucleic acid from ribosomes. A G factor and guanosine triphosphate-dependent reaction
20. Ribosome recycling by ribosome recycling factor (RRF) - An important but overlooked step of protein biosynthesis
21. Dual functions of ribosome recycling factor in protein biosynthesis: Disassembling the termination complex and preventing translational errors
22. Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis
23. Ribosome-recycling factor: An essential factor for protein synthesis
24. Specific interaction of soluble RNA with polyribonucleic acid induced polysomes
25. The fourth step of protein synthesis: Disassembly of the post-termination complex is catalyzed by elongation factor G and ribosome recycling factor, RRF, a near perfect mimic of tRNA
26. Specific binding of sRNA to ribosomes: Effect of streptomycin
27. Binding of specific sRNA to template ribosome complex: Effect of proteolytic enzymes
28. Dissociation rate of cognate peptidyl-tRNA from the A-site of hyper-accurate and error-prone ribosomes
29. Novel roles for classical factors at the interface between translation termination and initiation
30. Crystal structure of the ribosome recycling factor from Escherichia coli
31. Formation of a complex between GTP, G factor and ribosomes as an intermediate of ribosome-dependent GTPase reaction
32. Affinities of tRNA binding sites of ribosomes from Escherichia coli
33. Release of transfer RNA during peptide chain elongation
34. Interaction of kanamycin and related antibiotics with the large subunit of ribosomes and the inhibition of translocation
35. Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA
36. The inhibition of ribosomal translocation by viomycin
37. Sedimentation studies on aminoacyl-sRNA synthetase and activation of aminoacyl-sRNA transfer factor
38. RNA codewords and protein synthesis: The effect of trinucleotides upon the binding of sRNA to ribosomes
39. Studies on the structure and function of ribosomes by combined use of chemical probing and X-ray crystallography
40. Requirement for ribosome-releasing factor for the release of ribosomes at the termination codon
41. Translational coupling during expression of the tryptophan operon of Escherichia coli
42. Conformational switch in the decoding region of 16S rRNA during aminoacyl-tRNA selection on the ribosome
43. Release factor RF3 abolishes competition between release factor RF1 and ribosome recycling factor (RRF) for a ribosome binding site
44. Studies on the formation of transfer ribonucleic acid-ribosome complexes. V. On the function of a soluble transfer factor in protein synthesis
45. Studies on the formation of transfer ribonucleic acid-ribosome complexes. VI. Oligopeptide synthesis and translocation on ribosomes in the presence and absence of soluble transfer factors
46. Specific interaction between the ribosome recycling factor and the elongation factor G from Mycobacterium tuberculosis mediates peptidyl-tRNA release and ribosome recycling in Escherichia coli
47. The third ribosomal tRNA-binding site, the E site, is occupied in native polysomes
48. Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome
49. Thiostrepton inhibits the turnover but not the GTPase of elongation factor G on the ribosome
50. Translation of phage Qβ mRNA: A test of the two-site model for ribosomal function
51. Streptomycin preferentially perturbs ribosomal proofreading
52. Crystal structure of Thermotoga maritima ribosome recycling factor: A tRNA mimic
53. How does the mRNA pass through the ribosome?
54. Cell-free translation reconstituted with purified components
55. Isolation and analysis of ribosomes from prokaryotes, eukaryotes and organelles
56. Ribosomal translocation: Facts and models
57. Arrangement of tRNAs in pre- and post-translocational ribosomes revealed by electron cryomicroscopy
58. Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation
59. Nucleotide sequences of the ribosomal binding sites of bacteriophage R17 RNA
60. A factor required for ribosome dissociation in Escherichia coli
61. Mechanism of protein synthesis inhibition by fusidic acid and related antibiotics
62. Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch
63. Solution structure of the ribosome recycling factor from Aquifex aeolicus
64. Crystal structure of the ribosome at 5.5 Å resolution
65. The path of messenger RNA through the ribosome