Myosin-1c Interacts with Hair-Cell Receptors through Its Calmodulin-Binding IQ Domains

Journal of Neuroscience

Volumn 22, Issue 7, 2002, Pages 2487-2495

  Cyr, Janet L ^a,b   Dumont, Rachel A ^a   Gillespie, Peter G ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b WEST VIRGINIA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adaptation; Calmodulin; Hair bundle; Hair cell; IQ domain; Myo1c; Myosin 1c; Myosin 1 ; Stereocilia; Transduction

Indexed keywords

CALCIUM; CALMODULIN; CALMODULIN BINDING PROTEIN; MYOSIN; MYOSIN 1C; RECEPTOR; UNCLASSIFIED DRUG; CELL SURFACE RECEPTOR; ISOPROTEIN; LIGAND; MYOSIN I;

ADAPTATION; ANIMAL CELL; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; HAIR CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING; STEREOCILIUM; ANIMAL; BACULOVIRUS; CELL CULTURE; CELL SEPARATION; CYTOLOGY; DRUG EFFECT; EUKARYOTIC FLAGELLUM; FLUORESCENT ANTIBODY TECHNIQUE; FROG; GENE DELETION; GENETICS; HEARING; IN VITRO STUDY; INTRACELLULAR FLUID; METABOLISM; PHYSIOLOGY; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; VESTIBULE; XENOPUS;

ADAPTATION, PHYSIOLOGICAL; ANIMALS; BACULOVIRIDAE; BINDING SITES; CALCIUM; CALMODULIN; CELL SEPARATION; CELLS, CULTURED; CILIA; FLUORESCENT ANTIBODY TECHNIQUE; HAIR CELLS; HEARING; INTRACELLULAR FLUID; LIGANDS; MUTAGENESIS, SITE-DIRECTED; MYOSIN TYPE I; PROTEIN BINDING; PROTEIN ISOFORMS; PROTEIN STRUCTURE, TERTIARY; RANA CATESBEIANA; RECEPTORS, CELL SURFACE; SACCULE AND UTRICLE; SEQUENCE DELETION; SIGNAL TRANSDUCTION; XENOPUS;

EID: 0036547070     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.22-07-02487.2002     Document Type: Article

References (44)

1. Aruffo A, Stamenkovic I, Melnick M, Underhill CB, Seed B (1990) CD44 is the principal cell surface receptor for hyaluronate. Cell 61:1303-1313.
2. Assad JA, Hacohen N, Corey DP (1989) Voltage dependence of adaptation and active bundle movement in bullfrog saccular hair cells. Proc Natl Acad Sci USA 86:2918-2922.
3. Beningo KA, Lillie SH, Brown SS (2000) The yeast kinesin-related protein Smylp exerts its effects on the class V myosin Myo2p via a physical interaction. Mol Biol Cell 11:691-702.
4. Berg JS, Powell BC, Cheney RE (2001) A millennial myosin census. Mol Biol Cell 12:780-794.
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
6. Devos R, Richards JG, Campfield LA, Tartaglia LA, Guisez Y, van der Heyden J, Travernier J, Plaetinck G, Burn P (1996) OB protein binds specifically to the choroid plexus of mice and rats. Proc Natl Acad Sci USA 93:5668-5673.
7. 2+-ATPase isoform 2a is the PMCA of hair bundles. J Neurosci 21:5066-5078.
8. Flanagan JG, Leder P (1990) The kit ligand: a cell surface molecule altered in steel mutant fibroblasts. Cell 63:185-194.
9. Gillespie PG, Corey DP (1997) Myosin and adaptation by hair cells. Neuron 19:955-958.
10. Gillespie PG, Hudspeth AJ (1991) High-purity isolation of bullfrog hair bundles and subcellular and topological localization of constituent proteins. J Cell Biol 112:625-640.
11. Gillespie PG, Wagner MC, Hudspeth AJ (1993) Identification of a 120 kd hair-bundle myosin located near stereociliary tips. Neuron 11:581-594.
12. 6-modified ADP analogs. J Biol Chem 274:31373-31381.
13. 2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography. Biochem Biophys Res Commun 104:830-836.
14. Hasson T, Gillespie PG, Garcia JA, MacDonald RB, Zhao Y, Yee AG, Corey DP (1997) Unconventional myosins in inner-ear sensory epithelia. J Cell Biol 137:1287-1307.
15. Holt JR, Corey DP (2000) Two mechanisms for transducer adaptation in vertebrate hair cells. Proc Natl Acad Sci USA 97:11730-11735.
16. Holt JR, Gillespie SKH, Provance DW, Shah K, Shokat KM, Corey DP, Mercer JA, Gillespie PG (2002) A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells. Cell 108:371-381.
17. Hudspeth AJ (1982) Extracellular current flow and the site of transduction by vertebrate hair cells. J Neurosci 2:1-10.
18. Hudspeth AJ (1989) How the ear's works work. Nature 341:397-404.
19. 32P-labeled protein, applied to HIV-1 Gag and Rev. Gene 162:235-237.
20. Jung G, Remmert K, Wu X, Volosky JM, Hammer III JA (2001) The Dictyostelium CARMIL protein links capping protein and the Arp2/3 complex to type I myosins through their SH3 domains. J Cell Biol 153:1479-1497.
21. Jurado LA, Chockalingam PS, Jarrett HW (1999) Apocalmodulin. Physiol Rev 79:661-682.
22. Knowles GC, McCulloch CA (1992) Simultaneous localization and quantification of relative G and F actin content: optimization of fluorescence labeling methods. J Histochem Cytochem 40:1605-1612.
23. Kolodkin AL, Levengood DV, Rowe EG, Tai YT, Giger RJ, Ginty DD (1997) Neuropilin is a semaphorin III receptor. Cell 90:753-762.
24. Kussel-Andermann P, El-Amraoui A, Safieddine S, Hardelin JP, Nouaille S, Camonis J, Petit C (2000a) Unconventional myosin VIIA is a novel A-kinase-anchoring protein. J Biol Chem 275:29654-29659.
25. Kussel-Andermann P, El-Amraoui A, Safieddine S, Nouaille S, Perfettini I, Lecuit M, Cossart P, Wolfrum U, Petit C (2000b) Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex. EMBO J 19:6020-6029.
26. Lapierre LA, Kumar R, Hales CM, Navarre J, Bhartur SG, Burnette JO, Provance Jr DW, Mercer JA, Bähler M, Goldenring JR (2001) Myosin Vb is associated with plasma membrane recycling systems. Mol Biol Cell 12:1843-1857.
27. 2+ entry through mechanosensitive channels localizes the site of mechanoelectrical transduction in hair cells. Proc Natl Acad Sci USA 92:10297-10301.
28. 2+ concentration in hair-cell stereocilia. J Neurosci 18:6300-6318.
29. Mermall V, Post PL, Mooseker MS (1998) Unconventional myosins in cell movement, membrane traffic, and signal transduction. Science 279:527-533.
30. Metcalf AB, Chelliah Y, Hudspeth AJ (1994) Molecular cloning of a myosin Iβ isozyme that may mediate adaptation by hair cells of the bullfrog's internal ear. Proc Natl Acad Sci USA 91:11821-11825.
31. Muller U, Wang D, Denda S, Meneses JJ, Pedersen RA, Reichardt LF (1997) Integrin alpha8beta1 is critically important for epithelial-mesenchymal interactions during kidney morphogenesis. Cell 88:603-613.
32. Nakazawa K (2001) Ultrastructural localization of calmodulin in gerbil cochlea by immunogold electron microscopy. Hear Res 151:133-140.
33. O'Reilly DR, Miller LK, Luckow VA (1994) Baculovirus expression vectors: a laboratory manual. New York: Oxford UP.
34. Ogata Y, Slepecky NB (1998) Immunocytochemical localization of calmodulin in the vestibular end-organs of the gerbil. J Vestib Res 8:209-216.
35. Pack AK, Slepecky NB (1995) Cytoskeletal and calcium-binding proteins in the mammalian organ of Corti: cell type-specific proteins displaying longitudinal and radial gradients. Hear Res 91:119-135.
36. Pardue RL, Kaetzel MA, Hahn SH, Brinkley BR, Dedman JR (1981) The identification of calmodulin-binding sites on mitochondria in cultured 3T3 cells. Cell 23:533-542.
37. Reizes O, Barylko B, Li C, Sudhof TC, Albanesi JP (1994) Domain structure of a mammalian myosin Iβ. Proc Natl Acad Sci USA 91:6349-6353.
38. Rhoads AR, Friedberg F (1997) Sequence motifs for calmodulin recognition. FASEB J 11:331-340.
39. Shepherd GMG, Barres BA, Corey DP (1989) "Bundle-blot" purification and initial protein characterization of hair cell stereocilia. Proc Natl Acad Sci USA 86:4973-4977.
40. Sherr EH, Joyce MP, Greene LA (1993) Mammalian myosin Iα, Iβ, and Iγ: new widely expressed genes of the myosin I family. J Cell Biol 120:1405-1416.
41. Solc CK, Derfler BH, Duyk GM, Corey DP (1994) Molecular cloning of myosins from bullfrog saccular macula: a candidate for the hair cell adaptation motor. Auditory Neurosci 1:63-75.
42. 2+-stimulated binding of myosin I to phosphatidylserine concerted with calmodulin dissociation. J Biol Chem 267:3445-3454.
43. Walker RG, Hudspeth AJ, Gillespie PG (1993) Calmodulin and calmodulin-binding proteins in hair bundles. Proc Natl Acad Sci USA 90:2807-2811.
44. Welsh MJ, Aster JC, Ireland M, Alcala J, Maisel H (1982) Calmodulin binds to chick lens gap junction protein in a calcium-independent manner. Science 216:642-644.