A thermostable L-aminoacylase from Thermococcus litoralis: Cloning, overexpression, characterization, and applications in biotransformations

Extremophiles

Volumn 6, Issue 2, 2002, Pages 111-122

  Toogood, Helen S ^a   Hollingsworth, Edward J ^a   Brown, Rob C ^b   Taylor, Ian N ^b   Taylor, Stephen J C ^b   McCague, Ray ^b   Littlechild, Jennifer A ^a  

^a UNIVERSITY OF EXETER   (United Kingdom)

^b Chirotech Technology Ltd   (United Kingdom)

Author keywords

Biotransformations; Carboxypeptidase; L Aminoacylase; Substrate specificity; Thermococcus litoralis; Thermostable

Indexed keywords

ESCHERICHIA COLI; PYROCOCCUS HORIKOSHII; SULFOLOBUS SOLFATARICUS; THERMOCOCCUS; THERMOCOCCUS LITORALIS;

AMIDASE; AMINOACYLASE I; METAL; RECOMBINANT PROTEIN; SOLVENT;

AMINO ACID SEQUENCE; ARTICLE; BIOTRANSFORMATION; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; SEQUENCE HOMOLOGY; TEMPERATURE; THERMOCOCCUS;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; BIOTRANSFORMATION; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, ARCHAEAL; METALS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SOLVENTS; SUBSTRATE SPECIFICITY; TEMPERATURE; THERMOCOCCUS;

EID: 0036545508     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s007920100230     Document Type: Article

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