Studies on kinetic parameters and stability of aminoacylase in non-conventional media

Journal of Biotechnology

Volumn 66, Issue 1, 1998, Pages 69-73

  Boross, L ^a   Kosary J ^a   Stefanovits Bányai, É ^a   Sisak, C ^b   Szajani B ^c  

^a UNIV OF HORT AND FOOD INDUSTRY   (Hungary)

^b UNIVERSITY OF PANNONIA   (Hungary)

^c Covent Co Inc ^*  (Hungary)

Author keywords

Aminoacylase; Dioxane; Kinetic parameters; N,N dimethylformamide; Non conventional media; Stability

Indexed keywords

BINARY MIXTURES; CATALYSIS; CATALYST ACTIVITY; CATALYST DEACTIVATION; CONFORMATIONS; ENZYME INHIBITION; NITROGEN COMPOUNDS; SOLVENTS;

AMINOACYLASE; DIMETHYLFORMAMIDE; DIOXANE;

ENZYME KINETICS;

AMINOACYLASE; DIOXANE; HYDROLASE; N,N DIMETHYLFORMAMIDE; ORGANIC SOLVENT; WATER;

ACYLATION; BIOTECHNOLOGY; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STABILITY; NONHUMAN; PRIORITY JOURNAL; SOLVATION;

AMIDOHYDROLASES; DIMETHYLFORMAMIDE; DIOXANES; ENZYME STABILITY; KINETICS; SOLVENTS;

EID: 0344836063     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00158-8     Document Type: Conference Paper

References (9)

1. Bell G., Halling P.J., Moore B.D., Patridge J., Rees D.G. Biocatalyst behaviour in low water systems. Trends Biotechnol. 13:1995;468-473.
2. Carrea G., Ottolina G., Riva S. Role of solvents in the control of enzyme selectivity in organic media. Trends Biotechnol. 13:1995;63-70.
3. Farber K., Franssen M.C. Prospects for the increased application of biocatalysts in organic transformations. Trends Biotechnol. 13:1993;63-70.
4. Iborra J.L., Obon J.M., Manjon A., Canovas S.M. Water concentration and activity effects on aminoacylase in aqueous/organic one-liquid-phase systems. Biocatalysis. 7:1992;37-48.
5. Khmelnitsky G.L., Levashov A.V., Klyachko N.L., Martinek K. Engineering biocatalytic systems in organic media with low water content. Enzyme Microbiol. Biotechnol. 10:1988;710-724.
6. Kosáry J., Sisak C., Szajáni B., Boross L. Acylation of amino acids by aminoacylase in non-conventional media. Biocatalysis. 11:1994;329-337.
7. Simon L.M., László K., Vértesi A., Bagi K., Szajáni B. Stability of hydrolytic enzymes in water-organic solvent systems. J. Mol. Biocatal. 4B:1998;41-45.
8. Szajáni B., Kiss A., Boross L. Investigation of the active center and catalytic mechanism of porcine kidney aminoacylase: a model of the active center. Acta Biochim. Biophys. Acad. Sci. Hung. 15:1980;29-37.
9. Terradas F., Feston-Henry M., Fitzpatrick P.A., Klibanov A.M. Marked dependence of enzyme prochiral selectivity on solvent. J. Am. Chem. Soc. 115:1993;390-396.