A model system for optimising the selection of membrane antigen-specific human antibodies on intact cells using phage antibody display technology

Journal of Immunological Methods

Volumn 262, Issue 1-2, 2002, Pages 191-204

  Hegmans, J P J J ^a   Radosevic, K ^a   Voerman, J S A ^a   Burgers, J A ^a   Hoogsteden, H C ^a   Prins, J B ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Fluorescein DHPE; Intact cells; Model system; Optimisation; Phage antibody display

Indexed keywords

ANTIBODY; FLUORESCENT DYE; MEMBRANE ANTIGEN; PHOSPHOLIPID DERIVATIVE;

ADULT; ANTIBODY ISOLATION; ANTIBODY SPECIFICITY; ARTICLE; CELL LABELING; CELL MEMBRANE; CELL TYPE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EXPERIMENTAL MODEL; HUMAN; HUMAN CELL; INCUBATION TIME; MALE; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROCESS OPTIMIZATION; TECHNOLOGY;

ANTIBODIES; ANTIBODY SPECIFICITY; ANTIGENS, SURFACE; CELL LINE; HUMANS; MALE; MIDDLE AGED; PEPTIDE LIBRARY; REPRODUCIBILITY OF RESULTS;

FILAMENTOUS BACTERIOPHAGE;

EID: 0036537832     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00005-4     Document Type: Article

References (32)

1. A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells
2. Anti-melanoma antibodies from melanoma patients immunized with genetically modified autologous tumor cells: Selection of specific antibodies from single-chain Fv fusion phage libraries
3. Isolation of anti-mesothelin antibodies from a phage display library
4. Isolation of a high-affinity stable single-chain Fv specific for mesothelin from DNA-immunized mice by phage display and construction of a recombinant immunotoxin with anti-tumor activity
5. Streptabody, a high avidity molecule made by tetramerization of in vivo biotinylated, phage display-selected scFv fragments on streptavidin
6. Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions
7. Rapid selection of cell subpopulation-specific human monoclonal antibodies from a synthetic phage antibody library
8. New perspectives on recombinant human antibodies
9. Isolation of high affinity human antibodies directly from large synthetic repertoires
10. Human single-chain Fv antibodies to MUC1 core peptide selected from phage display libraries recognize unique epitopes and predominantly bind adenocarcinoma
11. Designing and optimizing library selection strategies for generating high-affinity antibodies
12. Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains
13. Antibody phage display technology and its applications
14. Malignant pleural mesothelioma
15. Antibodies to human fetal erythroid cells from a non-immune phage antibody library
16. Isolation of tumor cell-specific single-chain Fv from immunized mice using phage-antibody libraries and the re-construction of whole antibodies from these antibody fragments
17. Generation and selection of monoclonal antibodies, single-chain Fv and antibody fusion phage specific for human melanoma-associated antigens
18. Isolation of human tumor-specific antibodies by selection of an antibody phage library on melanoma cells
19. Stochastic modeling and optimization of phage display
20. Phage-display library selection of high-affinity human single-chain antibodies to tumor-associated carbohydrate antigens sialyl Lewisx and Lewisx
21. Phage antibodies: Filamentous phage displaying antibody variable domains
22. Model systems to study the parameters determining the success of phage antibody selections on complex antigens
23. The use of phage display for the development of tumour targeting agents
24. A model system for detection and isolation of a tumor cell surface antigen using antibody phage display
25. Combinatorial antibodies against human malignant melanoma
26. New protein engineering approaches to multivalent and bispecific antibody fragments
27. In vitro and in vivo characterization of a human anti-c-erbB-2 single-chain Fv isolated from a filamentous phage antibody library
28. Isolation of cell surface-specific human monoclonal antibodies using phage display and magnetically-activated cell sorting: Applications in immunohematology
29. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments
30. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library
31. An optimized method for cell-based phage display panning
32. Making antibodies by phage display technology