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0012070156
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note
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Covalent disulfides form rapidly in the absence of dithiothreitol (DTT), linking through the terminal PP thiol to either the thiol of C17 or the PP thiol of another holo-ACP. In these forms, ACP is catalytically inactive. The use of a C17S mutant and preincubation with DTT avoids these pitfalls. See ref. [12] for more details.
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0012035942
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note
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At 5mM 5 b more acyl ACP and ACP SNAC was evident, but addition of MCAT made no detectable difference.
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28
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0029878720
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