A new nonhydrolyzable reactive cAMP analog, (Sp)-adenosine-3′,5′-cyclic-S-(4-bromo-2,3-dioxobutyl) monophosphorothioate irreversibly inactivates human platelet cGMP-inhibited cAMP phosphodiesterase

Bioorganic Chemistry

Volumn 30, Issue 1, 2002, Pages 16-31

  Hung, S H ^b   Madhusoodanan, K S ^a   Beres, J A ^a   Boyd, R L ^b   Baldwin, J L ^b   Zhang, W ^b   Colman, R W ^b   Colman, R F ^a  

^a UNIVERSITY OF DELAWARE   (United States)

^b TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

1,4 dibromobutanedione; Adenosine 3 ,5 cyclic monophosphorothioate; Affinity labeling; cAMP; cGMP; Cyclic nucleotide phosphodiesterases; PDE3A; Platelets

Indexed keywords

1,4 DIBROMOBUTANEDIONE; ADENOSINE 3',5' CYCLIC (4 BROMO 2,3 DIOXOBUTYL)PHOSPHOROTHIOATE; ADENOSINE 3',5' CYCLIC PHOSPHOROTHIOLATE; CYCLIC AMP DERIVATIVE; CYCLIC AMP PHOSPHODIESTERASE; CYCLIC NUCLEOTIDE PHOSPHODIESTERASE; UNCLASSIFIED DRUG;

AFFINITY LABELING; ARTICLE; BINDING SITE; COMPETITIVE INHIBITION; DISSOCIATION CONSTANT; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME PURIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SECOND MESSENGER; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; THROMBOCYTE FUNCTION;

EID: 0036479121     PISSN: 00452068     EISSN: None     Source Type: Journal    
DOI: 10.1006/bioo.2001.1226     Document Type: Article

References (30)