Implication of His68 in the substrate site of Bacillus subtilis adenylosuccinate lyase by mutagenesis and affinity labeling with 2- [(4- bromo-2,3-dioxobutyl)thio]adenosine 5'-monophosphate

Biochemistry

Volumn 37, Issue 23, 1998, Pages 8481-8489

  Lee, Tom T ^a   Worby, Carolyn ^b   Bao, Zhao Qin ^b   Dixon, Jack E ^b   Colman, Roberta F ^a  

^a UNIVERSITY OF DELAWARE   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 [(4 BROMO 2,3 DIOXOBUTYL)THIO]ADENOSINE 5' PHOSPHATE; ADENOSINE DERIVATIVE; ADENOSINE PHOSPHATE; ADENYLOSUCCINATE LYASE; ADENYLOSUCCINIC ACID; ALANINE; ARGININE; BACTERIAL ENZYME; FUMARIC ACID; HISTIDINE; ISOLEUCINE; MUTANT PROTEIN; SUCCINIC ACID DERIVATIVE; UNCLASSIFIED DRUG; 2 ((4 BROMO 2,3 DIOXOBUTYL)THIO) ADENOSINE 3'5' CYCLIC MONOPHOSPHATE; 2-((4-BROMO-2,3-DIOXOBUTYL)THIO)-ADENOSINE 3'5'-CYCLIC MONOPHOSPHATE; CARBON; CYCLIC AMP; DRUG DERIVATIVE; NUCLEOTIDE;

AFFINITY LABELING; ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; ENZYME SUBSTRATE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITE; CHEMISTRY; DRUG EFFECT; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PEPTIDE MAPPING;

ADENYLOSUCCINATE LYASE; AFFINITY LABELS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARGININE; BACILLUS SUBTILIS; BINDING SITES; CARBON RADIOISOTOPES; CYCLIC AMP; ENZYME ACTIVATION; HISTIDINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE MAPPING; SUBSTRATE SPECIFICITY; THIONUCLEOTIDES;

EID: 0032499536     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9805339     Document Type: Article

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