Flow cytometric determination of intracytoplasmic Wiskott-Aldrich syndrome protein in peripheral blood lymphocyte subpopulations

Journal of Immunological Methods

Volumn 260, Issue 1-2, 2002, Pages 195-205

  Kawai, S ^a   Minegishi, M ^a,b   Ohashi, Y ^a   Sasahara, Y ^a   Kumaki, S ^a   Konno, T ^c   Miki, H ^d   Derry, J ^e   Nonoyama, S ^f   Miyawaki, T ^g   Horibe, K ^h   Tachibana, N ⁱ   Kudoh, E ^j   Yoshimura, Y ^k   Izumikawa, Y ^l   Sako, M ^m   Tsuchiya, S ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY HOSPITAL   (Japan)

^c Miyagi Cancer Center   (Japan)

^d THE UNIVERSITY OF TOKYO   (Japan)

^e AMGEN INC   (United States)

^f TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^g TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY   (Japan)

^h NAGOYA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

ⁱ AOMORI PREFECTURAL CENTRAL HOSPITAL   (Japan)

^j HIROSAKI NATIONAL HOSPITAL   (Japan)

^k Yamagata Prefectural Shinjo Hospital   (Japan)

^l UNIVERSITY OF THE RYUKYUS SCHOOL OF MEDICINE   (Japan)

^m OSAKA CITY GENERAL HOSPITAL   (Japan)

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Author keywords

Flow cytometry; Monoclonal antibody; WASP; Wiskott Aldrich syndrome; X linked thrombocytopenia

Indexed keywords

CD19 ANTIGEN; CD3 ANTIGEN; CD56 ANTIGEN; WISKOTT ALDRICH SYNDROME PROTEIN;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL LINE; CELL SURFACE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; EPSTEIN BARR VIRUS; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; INTERMETHOD COMPARISON; LYMPHOBLASTOID CELL; LYMPHOCYTE SUBPOPULATION; MALE; MONONUCLEAR CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; THROMBOCYTOPENIA; VIRUS TRANSFORMATION; WESTERN BLOTTING; WISKOTT ALDRICH SYNDROME;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE, TRANSFORMED; CYTOPLASM; FEMALE; FLOW CYTOMETRY; HUMANS; LYMPHOCYTES; MICE; MICE, INBRED BALB C; PROTEINS; THROMBOCYTOPENIA; WISKOTT-ALDRICH SYNDROME; WISKOTT-ALDRICH SYNDROME PROTEIN;

HUMAN HERPESVIRUS 4; VESPIDAE;

EID: 0036469993     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00549-X     Document Type: Article

References (38)

1. A growth factor for B cell hybridomas produced by human monocytes
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding
3. Wiskott-Aldrich syndrome: An immunologic deficiency disease involving the afferent limb of immunity
4. Isolation of a novel gene mutated in Wiskott-Aldrich syndrome
5. WASP gene mutations in Wiskott-Aldrich syndrome and X-linked thrombocytopenia
6. Deficient expression of Bruton's tyrosine kinase in monocytes from X-linked agammaglobulinemia as evaluated by a flow cytometric analysis and its clinical application to carrier detection
7. Activation by Cdc 42 and PIP2 of Wiskott-Aldrich Syndrome protein (WASp) stimulates actin nucleation by Arp 2/3 complex
8. An Ia-like antigen positive null cell leukemia cell line (THP-5) lacking in the stimulating capacity in autologous and allogeneic mixed lymphocyte reaction
9. Establishment and characterization of a novel human immature megakaryoblastic leukemia cell line, M-MOK, dependent on fibroblasts for its viability
10. Mutations of the WASP gene in 10 Japanese patients with Wiskott-Aldrich syndrome and X-linked thrombocytopenia
11. X-linked thrombocytopenia identified by flow cytometric demonstration of defective Wiskott-Aldrich syndrome protein in lymphocytes
12. Identification of WASP mutations in patients with Wiskott-Aldrich syndrome and isolated thrombocytopenia reveals allelic heterogeneity at the WAS locus
13. Identification of mutations in the Wiskott-Aldrich syndrome gene and characterization of a polymorphic dinucleotide repeat at DXS6940, adjacent to the disease gene
14. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm
15. N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases
16. Establishment of five human malignant non-T lymphoid cell lines and mixed lymphocyte-tumor reaction
17. Characterization of a precursor T-cell line (THP-6) with rearranged T-cell receptor β chain gene
18. Erythropoietin-dependent induction of hemoglobin synthesis in a cytokine-dependent cell line M-TAT
19. A human CD4-CD8-T-cell receptor αβ+T leukemic cell line undergoing phytohemagglutinin-ind ed apoptosis
20. Structure and function of Cas-L, a 105-kD crk-associated substrate-related protein that is involved in β1 integrin-mediated signaling in lymphocytes
21. Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: Selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture
22. Isolation and characterization of two monoclonal antibodies that recognize different epitopes of the human c-kit receptor
23. The effector cells in human peripheral blood mediating mitogen-induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity
24. The Wiskott-Aldrich syndrome: Studies of lymphocytes, granulocytes, and platelets
25. Three monoclonal antibodies against human LFA-1 α and β chains with different biological activities
26. Expression of Wiskott-Aldrich Syndrome Protein (WASP) gene during hematopoietic differentiation
27. Defects in Wiskott-Aldrich syndrome blood cells
28. Novel mutations, no detectable mRNA and familial genetic analysis of the Wiskott-Aldrich syndrome protein gene in six Japanese patients with Wiskott-Aldrich syndrome
29. WASP levels in platelets and lymphocytes of Wiskott-Aldrich syndrome patients correlate with cell dysfunction
30. Studies of the expression of the Wiskott-Aldrich syndrome protein
31. A multi-institutional survey of the Wiskott-Aldrich syndrome
32. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1)
33. X-linked thrombocytopenia and Wiskott-Aldrich syndrome are allelic diseases with mutation in the WASP gene
34. High prevalence of nonsense, frame-shift and splice-site mutations in 16 patients with full-blown Wiskott-Aldrich syndrome
35. Flow cytometric analysis of Wiskott-Aldrich syndrome (WAS) protein in lymphocytes from WAS patients and their familial carriers
36. The Wiskott-Aldrich syndrome and X-linked congenital thrombocytopenia are caused by mutations of the same gene
37. Wiskott-Aldrich syndrome/X-linked thrombocytopenia: WASP gene mutations, protein expression, and phenotype