Real-time zymography and reverse zymography: A method for detecting activities of matrix metalloproteinases and their inhibitors using FITC-labeled collagen and casein as substrates

Analytical Biochemistry

Volumn 301, Issue 1, 2002, Pages 27-34

  Hattori, Shunji ^a,b   Fujisaki, Hitomi ^a   Kiriyama, Tomomi ^a   Yokoyama, Tsukao ^c   Irie, Shinkichi ^a,b  

^a NIPPI RESEARCH INSTITUTE OF BIOMATRIX   (Japan)

^b Japan Institute of Leather Research   (Japan)

^c Collagen Research Center ^*  (Japan)

Author keywords

Collagen; FITC; Matrix metalloproteinase; Real time; Tissue inhibitor of metalloproteinase; Zymography

Indexed keywords

COLLAGEN; GELS; SUBSTRATES; TISSUE;

FITC; MATRIX METALLOPROTEINASES; METALLOPROTEINASES; REAL TIME; ZYMOGRAPHY;

METALS;

CASEIN; COLLAGEN; FLUORESCEIN ISOTHIOCYANATE; MATRIX METALLOPROTEINASE; POLYACRYLAMIDE GEL; PROTEINASE INHIBITOR; TISSUE INHIBITOR OF METALLOPROTEINASE;

ACCURACY; ANALYTIC METHOD; ARTICLE; CHEMICAL REACTION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SUBSTRATE; FLUORESCENCE; INCUBATION TIME; PRIORITY JOURNAL; PROTEIN DEGRADATION; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; REAL TIME ZYMOGRAPHY; REVERSE ZYMOGRAPHY; ZYMOGRAPHY;

EID: 0036467706     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5479     Document Type: Article

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