Single-channel currents of the permeability transition pore from the inner mitochondrial membrane of rat liver and of a human hepatoma cell line

Cellular Physiology and Biochemistry

Volumn 12, Issue 5-6, 2002, Pages 269-278

  Loupatatzis, Christos ^b   Seitz, Gordon ^a   Schoenfeld, Peter ^a   Lang, Florian ^b   Siemen, Detlef ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Cyclosporin A; Inner mitochondrial membrane; Mitochondrial megachannel; Mitoplast; Patch clamp; Permeability transition pore

Indexed keywords

CALCIUM ION; CYCLOSPORIN A; EGTAZIC ACID; CALCIUM; CARRIER PROTEIN; CYCLOSPORIN; FAS ANTIGEN; ION CHANNEL; MITOCHONDRIAL PERMEABILITY TRANSITION PORE;

ANIMAL CELL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL LINE; CELL LYSATE; CONCENTRATION RESPONSE; CONDUCTANCE; CONTROLLED STUDY; DRUG EFFECT; ELECTRIC POTENTIAL; FEMALE; HEPATOMA CELL; HUMAN; HUMAN CELL; MEMBRANE PERMEABILITY; MITOCHONDRIAL MEMBRANE; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; ROOM TEMPERATURE; T LYMPHOCYTE; ANIMAL; CELL CULTURE; CELL MEMBRANE POTENTIAL; DOSE RESPONSE; ELECTROPHYSIOLOGY; INTRACELLULAR MEMBRANE; LEUKEMIA CELL LINE; LIVER CELL CARCINOMA; LIVER MITOCHONDRION; LIVER TUMOR; METABOLISM; PHYSIOLOGY;

ANIMALS; ANTIGENS, CD95; APOPTOSIS; CALCIUM; CARCINOMA, HEPATOCELLULAR; CYCLOSPORINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHYSIOLOGY; HUMANS; INTRACELLULAR MEMBRANES; ION CHANNELS; JURKAT CELLS; LIVER NEOPLASMS; MEMBRANE POTENTIALS; MITOCHONDRIA, LIVER; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; PATCH-CLAMP TECHNIQUES; RATS; TUMOR CELLS, CULTURED;

EID: 0036435713     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000067897     Document Type: Article

References (44)

1. Sorgato MC, Keller BU, Stühmer W: Patch-clamping of the inner mitochondrial membrane reveals a voltage-dependent ion channel. Nature 1987;330:498-500.
2. Sorgato MC, Moran O, De Pinto V, Keller BU and Stuehmer W: Further investigation on the high-conductance ion channel of the inner membrane of mitochondria. J Bioenerg Biomembr 1989;21:485-496.
3. Klitsch T, Siemen D: Inner mitochondrial membrane anion channel is present in brown adipocytes but is not identical with the uncoupling protein. J Membr Biol 1991;122:69-75.
4. Beavis AD: Properties of the inner membrane anion channel in intact mitochondria. J Bioenerg Biomembr 1992;24:77-90.
5. Borecky J, Jezek P, Siemen D: 108-pS channel in brown fat mitochondria might be identical to the inner membrane anion channel. J Biol Chem 1997;272:19282-19289.
6. Henry JP, Chich JF, Goldschmidt D, Thieffry M: Blockade of a mitochondrial cationic channel by an addressing peptide: An electrophysiological study. J Membr Biol 1989;112:139-147.
7. Inoue I, Nagase H, Kishi K and Higuti T: ATP-sensitive K+ channel in the mitochondrial inner membrane. Nature 1991;352:244-247.
8. Garlid KD: Cation transport in mitochondria - The potassium cycle. Biochim Biophys Acta 1996;1275:123-126.
9. Kinnally KW, Campo ML, Tedeschi H: Mitochondrial channel activity studied by patch-clamping mitoplasts. J Bioenerg Biomembr 1989;21:497-506.
10. Szabo I, Zoratti M: The mitochondrial permeability transition pore may comprise VDAC molecules. I. Binary structure and voltage dependence of the pore. FEBS Lett 1993;330:201-205.
11. Szabó I, De Pinto V, Zoratti M: The mitochondrial permeability transition pore may comprise VDAC molecules. II. The electrophysiological properties of VDAC are compatible with those of the mitochondrial megachannel. FEBS Lett 1993;330:206-210.
12. Szabo I, Zoratti M: The giant channel of the inner mitochondrial membrane is inhibited by cyclosporin A. J Biol Chem 1991;266:3376-3379.
13. Kroemer G, Zamzami N, Susin SA: Mitochondrial control of apoptosis. Immunol Today 1997;18:44-51.
14. 2+ . Biochemistry 1996;35:8483-8488.
15. Marzo I, Brenner C, Zamzami N, Jurgensmeier JM, Susin SA, Vieira HL, Prevost MC, Xie Z, Matsuyama S, Reed JC, Kroemer G: Bax and adenine nucleotide translocator cooperate in the mitochondrial control of apoptosis. Science 1998;281:2027-2031.
16. Halestrap AP: The mitochondrial permeability transition: Its molecular mechanism and role in reperfusion injury. Biochem Soc Symp 1999;66:181-203.
17. Shimizu S, Shinohara Y, Tsujimoto Y: Bax and bcl-xL independently regulate apoptotic changes of yeast mitochondria that require VDAC but not adenine nucleotide translocator. Oncogene 2000;19:4309-4318.
18. Lemasters JJ, Nieminen AL, Qian T, Trost LC, Elmore SP, Nishimura Y, Crowe RA, Cascio WE, Bradham CA, Brenner DA, Herman B: The mitochondrial permeability transition in cell death: A common mechanism in necrosis, apoptosis and autophagy. Biochim Biophys Acta 1998;1366:177-196.
19. Bernardi P: Mitochondrial transport of cations: Channels, exchangers, and permeability transition. Physiol Rev 1999;79:1127-1155.
20. Vieira HLA, Kroemer G: Pathophysiology of mitochondrial cell death control. CMLS Cell Mol Life Sci 1999;56:971-976.
21. Waterhouse, NJ, Goldstein JC, von Ahsen O, Schuler M, Newmeyer DD, Green DR: Cytochrome c maintains mitochondrial transmembrane potential and ATP generation after outer mitochondrial membrane permeabilization during the apoptotic process. J Cell Biol 2001;153:319-328.
22. Crompton M: Mitochondrial intermembrane junctional complexes and their role in cell death. J Physiol 2000;529:11-21.
23. Cotman CW, Anderson AJ: A potential role for apoptosis in neurodegeneration and Alzheimer's disease. Mol Neurobiol 1995;10:19-45.
24. Beal MF: Mitochondria, NO and neurodegeneration. Biochem Soc Symp 1999;66:43-54.
25. Berman SB and Hastings TG: Dopamine oxidation alters mitochondrial respiration and induces permeability transition in brain mitochondria: Implications for Parkinson's disease. J Neurochem 1999;73:1127-1137.
26. Lang F, Szabo I, Lepple-Wienhues A, Siemen D, Gulbins E: Physiology of receptor-mediated lymphocyte apoptosis. News Physiol Sci 1999;14:194-200.
27. Ichas F, Jouaville LS, Mazat JP: Mitochondria are excitable organelles capable of generating and conveying electrical and calcium signals. Cell 1997;89:1145-1153.
28. Gunter TE, Pfeiffer DR: Mechanisms by which mitochondria transport calcium. Am J Physiol 1990;258:C755-C786.
29. Smaili SS, Hsu YT, Youle R, Russel JT: Mitochondria in Ca2+ signaling and apoptosis. J Bioenergetics Biomembr 2000;32:35-46.
30. Lane D: How cells choose to die. Nature 2001;414:25-26.
31. Schönfeld P, Schild L, Kunz W: Long-chain fatty acids act as protonophoric uncouplers of oxidative phosphorylation in rat liver mitochondria. Biochim Biophys Acta 1989;977:266-272.
32. Brooks SP, Storey KB: Bound and determined: A computer program for making buffers of defined ion concentrations. Anal Biochem 1992;201:119-126.
33. 2+ -activated K channel of the BK-type in the inner mitochondrial membrane of a human glioma cell line. Biochem Biophys Res Commun 1999;257:549-554.
34. Szabó I, Bernardi P, Zoratti M: Modulation of the mitochondrial megachannel by divalent cations and protons. J Biol Chem 1992;267:2940-2946.
35. Lopez-Gomez FJ, Torres-Marquez ME, Moreno-Sanchez R: Control of oxidative phosphorylation in AS-30D hepatoma mitochondria. Int J Biochem 1993;25:373-377.
36. Kinnally KW, Antonenko YN, Zorov DB: Modulation of inner mitochondrial membrane channel activity. J Bioenerg Biomembr 1992;24:99-110.
37. Haworth RA, Hunter DR: Allosteric inhibition of the Ca2+-activated hydrophilic channel of the mitochondrial inner membrane by nucleotides. J Membr Biol 1980;54:231-236.
38. Gavin CE, Gunter KK, Gunter TE: Manganese and calcium transport in mitochondria: Implications for manganese toxicity. Neurotoxicology 1999;20:445-453.
39. Guo Q, Fu W, Sopher BL, Miller MW, Ware CB, Martin GM, Mattson MP: Increased vulnerability of hippocampal neurons to excitotoxic necrosis in presenilin-1 mutant knock-in mice. Nat Med 1999;5:101-106.
40. Bernardi P, Vassanelli S, Veronese P, Colonna R, Szabo I, Zoratti M: Modulation of the mitochondrial permeability transition pore. Effect of protons and divalent cations. J Biol Chem 1992;267:2934-2939.
41. Petronilli V, Penzo D, Scorrano L, Bernardi P, Di Lisa F: The mitochondrial permeability transition, release of cytochrome c and cell death. Correlation with the duration of pore openings in situ. J Biol Chem 2001;276:12030-12034.
42. Kroemer G, Reed JC: Mitochondrial control of cell death. Nat Med 2000;6:513-519.
43. Scaffidi C, Schmitz I, Zha J, Korsmeyer SJ, Krammer PH, Peter ME: Differential modulation of apoptosis sensitivity in CD95 type I and type II cells. J Biol Chem 1999;274:22532-22538.
44. nd Edn., Sinauer Assoc., Sunderland, MA 1991.