Purification, biochemical characterization and immunogenicity of SA5K, a secretion antigen of Mycobacterium tuberculosis

Scandinavian Journal of Immunology

Volumn 56, Issue 1, 2002, Pages 43-51

  Batoni, G ^a   Bottai, D ^a   Esin, S ^a   Florio, W ^a   Pardini, M ^a   Maisetta, G ^a   Freer, G ^a   Senesi, S ^a   Campa, M ^a  

^a UNIVERSITY OF PISA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BCG VACCINE; CD4 ANTIGEN; SA5K ANTIGEN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIGEN ANTIBODY REACTION; ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ANTIGEN SPECIFICITY; ARTICLE; BCG VACCINATION; CELL PROLIFERATION; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; HUMAN; HUMAN CELL; IMMUNOGENICITY; LUNG TUBERCULOSIS; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SERODIAGNOSIS; T LYMPHOCYTE; WESTERN BLOTTING;

ANTIGENS, BACTERIAL; B-LYMPHOCYTES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; MYCOBACTERIUM BOVIS; MYCOBACTERIUM TUBERCULOSIS; RECOMBINANT FUSION PROTEINS; T-LYMPHOCYTES; TUBERCULOSIS, PULMONARY;

EID: 0036423870     PISSN: 03009475     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3083.2002.01104.x     Document Type: Article

References (37)

1. Dye C, Scheele S, Dolin P, Pathania V, Raviglione MC. Global burden of tuberculosis. Estimated incidence prevalence and mortality by country. JAMA 1999;282:677-86.
2. Converse P J, Jones SL, Astemborski J, Vlahov D, Graham NMH. Comparison of a tuberculin interferon-γ assay with the tuberculin skin test in high-risk adults: Effect of immunodeficiency virus infection. J Infect Dis 1997;176:144-50.
3. Fine PE. The BCG story: Lessons from the past and implications for the future. Rev Infect Dis 1989;11:S353-9.
4. Milstien JB, Gibson JJ. Quality control of BCG vaccine by WHO: A review of factors that may influence vaccine effectiveness and safety. Bull World Health Organ 1990;68:93-108.
5. Andersen P. Host response and antigens involved in protective immunity to Mycobacterium tuberculosis. Scand J Immunol 1997;45:115-31.
6. Boesen H, Jensen BN, Wilcke T, Andersen P. Human T-cell responses to secreted antigen fractions of Mycobacterium tuberculosis. Infect Immun 1995;63:1491-7.
7. Kamath AT, Feng CG, Macdonald M, Briscoe H, Britton WJ. Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis. Infect Immun 1999;67:1702-7.
8. Huygen K, Content J, Denis O et al. Immunogenicity and protective efficacy of a tuberculosis DNA vaccine. Nat Med 1996;2:893-8.
9. Coler RN, Campos-Neto A, Ovendale P et al. Vaccination with the T cell antigen Mtb 8.4 protects against challenge with Mycobacterium tuberculosis. J Immunol 2001;166:6227-35.
10. Manca C, Lyashchenko K, Wiker HG, Usai D, Colangeli R, Gennaro ML. Molecular cloning, purification and serological characterization of MPT63, a novel antigen secreted by Mycobacterium tuberculosis. Infect Immun 1997;65:16-23.
11. Webb JR, Vedvick TJ, Alderson MR et al. Molecular cloning, expression, and immunogenicity of MTB12, a novel low-molecular-weight antigen secreted by Mycobacterium tuberculosis. Infect Immun 1998;66:4208-14.
12. Weldingh K, Rosenkrands I, Jacobsen S, Rasmussen PB, Elhay M J, Andersen P. Two-dimensional electrophoresis for analysis of Mycobacterium tuberculosis culture filtrate and purification and characterization of six novel proteins. Infect Immun 1998;66:3492-500.
13. Freer G, Florio W, Dalla Casa B et al. Identification and molecular cloning of a novel secretion antigen from Mycobacterium tuberculosis and Mycobacterium bovis BCG. Res Microbiol 1998;149:265-75.
14. Fernandes ND, Wu Q-L, Kong D, Puyang X, Garg S, Husson RN. A mycobacterial extracytoplasmatic sigma factor involved in survival following heat shock and oxidative stress. J Bacteriol 1999;181:4266-74.
15. Wiker HG, Michell SL, Hewinson RG, Spierings E, Nagai S, Harboe M. Cloning, expression and significance of MPT53 for identification of secreted proteins of Mycobacterium tuberculosis. Microb Pathog 1999;26:207-19.
16. Koul A, Choidas A, Treder M et al. Cloning and characterization of secretory tyrosine phosphatases of Mycobacterium tuberculosis. J Bacteriol 2000;182:5425-32.
17. Freer G, Florio W, Dalla Casa B et al. Subcellular localisation of antigens recognized by new monoclonal antibodies against proteins of Mycobacterium bovis bacillus Calmette-Guérin. FEMS Immunol Med Microbiol 1998;20:129-38.
18. Batoni G, Bottai D, Maisetta G et al. Involvement of the Mycobacterium tuberculosis secreted antigen SA5K in intracellular survival of recombinant Mycobacterium smegmatis. FEMS Microbiol Lett 2001;205:125-9.
19. Andersen P, Askgaard D, Ljungqvist L, Bennedsen J, Heron I. Proteins released from Mycobacterium tuberculosis during growth. Infect Immun 1991;59:1905-10.
20. Florio W, Freer G, Dalla Casa B et al. Comparative analysis of subcellular distribution of protein antigens in Mycobacterium bovis bacillus Calmette-Guérin. Can J Microbiol 1997;43:744-50.
21. Harboe M, Malin AS, Dockrell HS et al. B-cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tubercolosis. Infect Immun 1998;66:717-23.
22. Hewinson RG, Russell WP. Processing and secretion by Escherichia coli of a recombinant form of the immunogenic protein MPB70 of Mycobacterium tuberculosis. J Gen Microbiol 1993;139:1253-9.
23. Esin S, Batoni G, Kallenius G et al. Proliferation of distinct human T cell subsets in response to live, killed or soluble extracts of Mycobacterium tuberculosis and Myco. avium. Clin Exp Immunol 1996;104:419-25.
24. Batoni G, Esin S, Pardini M et al. Identification of distinct lymphocyte subsets responding to subcellular fractions of Mycobacterium bovis bacille Calmette-Guérin. Clin Exp Immunol 2000;119:270-9.
25. Dobos KM, Swiderek K, Khoo K-H, Brennan P J, Belisle JT. Evidence of glycosylation sites on the 45-kilodalton glycoprotein of Mycobacterium tuberculosis. Infect Immun 1995;63:2846-53.
26. Laqueyrerie A, Militzer P, Romain F, Eiglmeier K, Cole S, Marchal G. Cloning, sequencing and expression of the apa gene coding for the Mycobacterium tuberculosis 45/47-kilodalton secreted antigen complex. Infect Immun 1995;63:4003-10.
27. Wiker HG, Lyashchenko KP, Aksoy M et al. Immunochemical characterization of the MPB70/80 and MPB83 proteins of Mycobacterium bovis. Infect Immun 1998;66:1445-52.
28. Batoni G, Maisetta G, Florio W, Freer G, Campa M, Senesi S. Analysis of the Mycobacterium bovis hsp 60 promoter activity in recombinant Mycobacterium avium. FEMS Microbiol Lett 1998;169:117-24.
29. Coler RN, Skeiky YAW, Vedvick T et al. Molecular cloning and immunologic reactivity of a novel low molecular mass antigen of Mycobacterium tuberculosis. J Immunol 1998;161:2356-64.
30. Rhodes SG, Gavier-Widen D, Buddle BM et al. Antigen specificity in experimental bovine tuberculosis. Infect Immun 2000;68:2573-8.
31. Rook GA, Carswel JW, Stanford JL. Preliminary evidence for the trapping of antigen-specific lymphocytes in the lymphoid tissue of 'anergic' tuberculosis patients. Clin Exp Immunol 1976;26:129-32.
32. Vordermeier H-M, Harris DP, Friscia G et al. T cell repertoire in tuberculosis: Selective anergy to an immunodominant epitope of the 38-kD antigen in patients with active disease. Eur J Immunol 1992;22:2631-7.
33. Havlir DV, Wallis RS, Boom WH, Daniel TM, Chervenak K, Ellener JJ. Human immune response to Mycobacterium tuberculosis antigens. Infect Immun 1991;59:665-70.
34. Onwubalili JK, Scott GM, Robinson JA. Deficient immune interferon production in tuberculosis. Clin Exp Immunol 1985;59:405-13.
35. Bardana E J, JK, Farr RS, Minden P. Universal occurrence of antibodies to tubercle bacilli in sera from non-tuberculous and tuberculous individuals. Clin Exp Immunol 1973;13:65-77.
36. Laal S, Samanich KM, Sonnenberg MG, Zolla-Pazner S, Phadtare JM, Belisle JT. Human humoral responses to antigens of Mycobacterium tuberculosis: Immunodominance of high-molecular-mass antigens. Clin Diagn Lab Immun 1997;1:49-56.
37. Samanich K, Belisle JT, Laal S. Homogeneity of antibody responses in tuberculosis patients. Infect Immun 2001;69:4600-9.