Molecular cloning, expression, and immunogenicity of MTB12, a novel low- molecular-weight antigen secreted by Mycobacterium tuberculosis

Infection and Immunity

Volumn 66, Issue 9, 1998, Pages 4208-4214

  Webb, John R ^a,d   Vedvick, Thomas S ^b   Alderson, Mark R ^b   Guderian, Jeffrey A ^b   Jen, Shyian S ^b   Ovendale, Pamela J ^b   Johnson, Stephen M ^a,e   Reed, Steven G ^a,b,c   Skeiky, Yasir A W ^b  

^a INFECTIOUS DISEASE RESEARCH INSTITUTE   (United States)

^b GLAXOSMITHKLINE   (United States)

^c UNIVERSITY OF WASHINGTON   (United States)

^d UNIVERSITY OF OTTAWA   (Canada)

^e NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MYCOBACTERIUM ANTIGEN; PROTEIN MTB12; TUBERCULIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CULTURE MEDIUM; IMMUNE RESPONSE; IMMUNOBLOTTING; MOLECULAR CLONING; MOLECULAR WEIGHT; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; SEQUENCE ANALYSIS;

MYCOBACTERIUM BOVIS; MYCOBACTERIUM LEPRAE; MYCOBACTERIUM TUBERCULOSIS;

EID: 0031688910     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.9.4208-4214.1998     Document Type: Article

References (35)

1. Aldrich, C. J., A. De Cloux, A. S. Woods, R. J. Cotter, M. J. Soloski, and J. Forman. 1994. Identification of a Tap-dependent leader peptide recognized by alloreactive T cells specific for a class Ib antigen. Cell 79:649-658.
2. Andersen, P. 1994. Effective vaccination of mice against Mycobacterium tuberculosis infection with a soluble mixture of secreted mycobacterial proteins. Infect. Immun. 62:2536-2544.
3. Andersen, P., D. Askgaard, L. Ljungqvist, J. Bennedsen, and I. Heron. 1991. Proteins released from Mycobacterium tuberculosis during growth. Infect. Immun. 59:1905-1910.
4. Boesen, H., B. N. Jensen, T. Wilcke, and P. Andersen. 1995. Human T-cell responses to secreted antigen fractions of Mycobacterium tuberculosis. Infect. Immun. 63:1491-1497.
5. Braud, V., E. Y. Jones, and A. McMichael. 1997. The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9. Eur. J. Immunol. 27:1164-1169.
6. Brewer, T. F., and G. A. Colditz. 1995. Relationship between bacille Calmette-Guerin (BCG) strains and the efficacy of BCG vaccine in the prevention of tuberculosis. Clin. Infect. Dis. 20:126-135.
7. Colditz, G. A., C. S. Berkey, F. Mosteller, T. F. Brewer, M. E. Wilson, E. Burdick, and H. V. Fineberg. 1995. The efficacy of bacillus Calmette-Guerin vaccination of newborns and infants in the prevention of tuberculosis: meta-analyses of the published literature. Pediatrics 96:29-35.
8. Colditz, G. A., T. F. Brewer, C. S. Berkey, M. E. Wilson, E. Burdick. H. V. Fineberg, and F. Mosteller. 1994. Efficacy of BCG vaccine in the prevention of tuberculosis. Meta-analysis of the published literature. JAMA 271:698-702.
9. Content, J., A. de la Cuvellerie, L. De Wit, V. Vincent-Levy-Frebault, J. Ooms, and J. De Bruyn. 1991. The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis. Infect. Immun. 59:3205-3212.
10. De Bruyn, J., R. Bosnians, J. Nyabenda, and J. P. Van Vooren. 1989. Effect of zinc deficiency on the appearance of two immunodominant protein antigens (32 kDa and 65 kDa) in culture filtrates of mycobacteria. J. Gen. Microbiol. 135:79-84.
11. Dobos, K. M., K. H. Khoo, K. M. Swiderek, P. J. Brennan, and J. T. Belisle. 1996. Definition of the full extent of glycosylation of the 45-kilodalton glycoprotein of Mycobacterium tuberculosis. J. Bacteriol. 178:2498-2506.
12. Dobos, K. M., K. Swiderek, K. H. Khoo, P. J. Brennan, and J. T. Belisle. 1995. Evidence for giycosylation sites on the 45-kilodalton glycoprotein of Mycobacterium tuberculosis. Infect. Immun. 63:2846-2853.
13. Espitia, C., and R. Mantilla. 1989. Identification, isolation and partial characterization of Mycobacterium tuberculosis glycoprotein antigens. Clin. Exp. Immunol. 77:378-383.
14. Feinberg, A. P., and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
15. Garbe, T., D. Harris, M. Vordermeier, R. Lathigra, J. Ivanyi, and D. Young. 1993. Expression of the Mycobacterium tuberculosis 19-kilodalton antigen in Mycobacterium smegmatis: immunological analysis and evidence of glycosylation. Infect. Immun. 61:260-267.
16. Henderson, R. A., H. Michel, K. Sakaguchi, J. Shabanowitz, E. Appella, D. F. Hunt, and V. H. Engelhard. 1992. HLA-A2.1-associated peptides from a mutant cell line: a second pathway of antigen presentation. Science 255:1264-1266.
17. Hubbard, R. D., C. M. Flory, and F. M. Collins. 1992. Immunization of mice with mycobacterial culture filtrate proteins. Clin. Exp. Immunol. 87:94-98.
18. Hurley, S. S., G. A. Splitter, and R. A. Welch. 1987. Rapid lysis technique for mycobacterial species. J. Clin. Microbiol. 25:2227-2229.
19. Jacobs, R. F. 1994. Multiple-drug-resistant tuberculosis. Clin. Infect. Dis. 19: 1-10.
20. Kochi, A. 1991. The global tuberculosis situation and the new control strategy of the World Health Organization. Tubercle 72:1-6. (Editorial.)
21. Laqueyrerie, A., P. Militzer, F. Romain, K. Eiglmeier, S, Cole, and G. Marchal. 1995. Cloning, sequencing, and expression of the apa gene coding for the Mycobacterium tuberculosis 45/47-kilodalton secreted antigen complex. Infect. Immun. 63:4003-4010.
22. Launois, P., R. De Leys, M. N. Niang, A. Drowart, M. Andrien, P. Dierckx, J. L. Cartel, J. L. Sarthou, J. P. Van Vooren, and K. Huygen. 1994. T-cell-epitope mapping of the major secreted mycobacterial antigen Ag85A in tuberculosis and leprosy. Infect. Immun. 62:3679-3687.
23. Nagai, S., H. G. Wiker, M. Harboe, and M. Kinomoto. 1991. Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis. Infect. Immun. 59:372-382.
24. Oettinger, T., and A. B. Andersen. 1994. Cloning and B-cell-epitope mapping of MPT64 from Mycobacterium tuberculosis H37Rv. Infect. Immun. 62:2058-2064.
25. Orme, I. M., P. Andersen, and W. H. Boom. 1993. T cell response to Mycobacterium tuberculosis. J. Infect. Dis. 167:1481-1497.
26. Pal, P. G., and M. A. Horwitz. 1992. Immunization with extracellular proteins of Mycobacterium tuberculosis induces cell-mediated immune responses and substantial protective immunity in a guinea pig model of pulmonary tuberculosis. Infect. Immun. 60:4781-4792.
27. Roberts, A. D., M. G. Sonnenberg, D. J. Ordway, S. K. Furney, P. J. Brennan, J. T. Belisle, and I. M. Orme. 1995. Characteristics of protective immunity engendered by vaccination of mice with purified culture filtrate protein antigens of Mycobacterium tuberculosis. Immunology 85:502-508.
28. Roche, P. W., P. W. Peake, H. Billman-Jacobe, T. Doran, and W. J. Britton. 1994. T-cell determinants and antibody binding sites on the major mycobacterial secretory protein MPB59 of Mycobacterium bovis. Infect. Immun. 62: 5319-5326.
29. Roche, P. W., J. A. Triccas, D. T. Avery, T. Fifis, H. Billman-Jacobe, and W. J. Britton. 1994. Differential T cell responses to mycobacteria-secreted proteins distinguish vaccination with bacille Calmette-Guerin from infection with Mycobacterium tuberculosis. J. Infect. Dis. 170:1326-1330.
30. Romain, F., A. Laqueyrerie, P. Militzer, P. Pescher, P. Chavarot, M. Lagranderie, G. Auregan, M. Gheorghiu, and G. Marchal. 1993. Identification of a Mycobacterium bovis BCG 45/47-kilodalton antigen complex, an immunodominant target for antibody response after immunization with living bacteria. Infect. Immun. 61:742-750.
31. Skeiky, Y. A., D. R. Benson, M. Parsons, K. B. Elkon, and S. G. Reed. 1992. Cloning and expression of Trypanosoma cruzi ribosomal protein PO and epitope analysis of anti-PO autoantibodies in Chagas' disease patients. J. Exp. Med. 176:201-211.
32. Sonnenberg, M. G., and J. T. Belisle. 1997. Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry. Infect. Immun. 65:4515-4524.
33. Torres, M., T. Herrera, H. Villareal, E. A. Rich, and E. Sada. 1998. Cytokine profiles for peripheral blood lymphocytes from patients with active pulmonary tuberculosis and healthy household contacts in response to the 30-kilodalton antigen of Mycobacterium tuberculosis. Infect. Immun. 66:176-180.
34. Wei, M. L., and P. Cresswell. 1992. HLA-A2 molecules in an antigen-processing mutant cell contain signal sequence-derived peptides. Nature 356: 443-446.
35. Young, D. B., S. H. Kaufmann, P. W. Hermans, and J. E. Thole. 1992. Mycobacterial protein antigens: a compilation. Mol. Microbiol. 6:133-145.