Production of extracellular domain of human tissue factor using maltose-binding protein fusion system

Protein Expression and Purification

Volumn 26, Issue 2, 2002, Pages 229-234

  Guan, Ming ^a   Su, Bing ^a   Ye, Chuanzhong ^b   Lu, Yuan ^a  

^a FUDAN UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

Fusion expression; Recombinant protein; Tissue factor

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

CARRIER PROTEIN; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN; THROMBOPLASTIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; GENETICS; HUMAN; ISOLATION AND PURIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

BLOTTING, WESTERN; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; RECOMBINANT FUSION PROTEINS; THROMBOPLASTIN;

EID: 0036417348     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00537-5     Document Type: Article

References (25)

1. E.K. Spicer, R. Horton, L. Bloem, R. Bach, K.R. Williams, A. Guha, J. Kraus, T.C. Lin, Y. Nemerson, W.H. Konigsberg, Isolation of cDNA clones coding for human tissue factor: Primary structure of the protein and cDNA, Proc. Natl. Acad. Sci. USA 84 (1987) 5148-5152.
2. W. Ruf, B.M. Mueller, Tissue factor in cancer angiogenesis and metastasis, Curr. Opin. Hematol. 3 (1996) 379-384.
3. J. Contrino, G. Hair, D.L. Kreutzer, F.R. Rickles, In situ detection of tissue factor in vascular endothelial cells: Correlation with the malignant phenotype of human breast disease, Nat. Med. 2 (1996) 209-215.
4. W. Ruf, A. Rehemtulla, J.H. Morrissey, T.S. Edgington, Phospholipid-independent and -dependent interactions required for tissue factor receptor and cofactor function, J. Biol. Chem. 266 (1991) 2158-2166.
5. S. Krishnaswamy, K.A. Field, T.S. Edgington, J.H. Morrissey, K.G. Mann, Role of the membrane surface in the activation of human coagulation factor X, J. Biol. Chem. 267 (1992) 26110-26120.
6. S. Ran, B. Gao, S. Duffy, L. Watkins, N. Rote, P.E. Thorpe, Infarction of solid Hodgkin's tumors in mice by antibody-directed targeting of tissue factor to tumor vasculature, Cancer Res. 58 (1998) 4646-4653.
7. J.F. Rippmann, K. Pfizenmaier, R. Mattes, W.J. Rettig, D. Moosmayer, Fusion of the tissue factor extracellular domain to a tumour stroma specific single-chain fragment variable antibody results in an antigen-specific coagulation-promoting molecule, Biochem. J. 349 (2000) 805-812.
8. X. Huang, G. Molema, S. King, L. Watkins, T.S. Edgington, P.E. Thorpe, Tumor infarction in mice by antibody-directed targeting of tissue factor to tumor vasculature, Science 275 (1997) 547-550.
9. F. Nilsson, H. Kosmehl, L. Zardi, D. Neri, Targeted delivery of tissue factor to the ED-B domain of fibronectin, a marker of angiogenesis, mediates the infarction of solid tumors in mice, Cancer Res. 61 (2001) 711-716.
10. M. Guan, B. Su, Y. Lu, Quantitative reverse transcription-PCR measurement of tissue factor mRNA in glioma, Mol. Biotechnol. 20 (2002) 123-129.
11. S.D. Carson, Continuous chromogenic tissue factor assay: Comparison to clot-based assays and sensitivity established using pure tissue factor, Thromb. Res. Suppl. 47 (1987) 379-387.
12. B. Jude, B. Agraou, E.P. McFadden, S. Susen, C. Bauters, P. Lepelley, C. Vanhaesbroucke, P. Devos, A. Cosson, P. Asseman, Evidence for time-dependent activation of monocytes in the systemic circulation in unstable angina but not in acute myocardial infarction or in stable angina, Circulation 90 (1994) 1662-1668.
13. M. Guan, Z.M. Ni, Y. Lu, A simple method for the quantification of procoagulant activity of tissue factor, J. Clin. Hematol. 14 (2001) 166-168 (published in Chinese).
14. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
15. B.R. Zetter, On target with tumor blood vessel markers, Nat. Biotechnol. 15 (1997) 1243-1244.
16. C.V. Maina, P.D. Riggs, A.G. Grandea III, B.E. Slatko, L.S. Moran, J.A. Tagliamonte, L.A. McReynolds, C.D. Guan, An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein, Gene 71 (1988) 365-373.
17. M. Guan, Y.G. Wang, J.F. Dai, Y. Lu, Z.M. Ni, C.Q. Chen, Purification of extracellular domain of human tissue factor using GST fusion system, Chin. J. Biochem. Mol. Biol. 17 (2001) 35-38 (published in Chinese).
18. R.B. Kapust, D.S. Waugh, Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused, Protein Sci. 8 (1999) 1668-1674.
19. K. Nagai, M.F. Perutz, C. Poyart, Oxygen binding properties of human mutant hemoglobins synthesized in Escherichia coli, Proc. Natl. Acad. Sci. USA 82 (1985) 7252-7255.
20. R.A. Quinlan, R.D. Moir, M. Stewart, Expression in Escherichia coli of fragments of glial fibrillary acidic protein: Characterization, assembly properties and paracrystal formation, J. Cell Sci. 93 (1989) 71-83.
21. D. Eaton, H. Rodriguez, G.A. Vehar, Proteolytic processing of human factor VIII. Correlation of specific cleavages by thrombin, factor Xa, and activated protein C with activation and inactivation of factor VIII coagulant activity, Biochemistry 25 (1986) 505-512.
22. S.J. Wearne, Factor Xa cleavage of fusion proteins. Elimination of non-specific cleavage by reversible acylation, FEBS Lett. 263 (1990) 23-26.
23. A.R. Rezaie, M.M. Fiore, P.F. Neuenschwander, C.T. Esmon, J.H. Morrissey, Expression and purification of a soluble tissue factor fusion protein with an epitope for an unusual calcium-dependent antibody, Protein Expr. Purif. 3 (1992) 453-460.
24. E. Waxman, J.B. Ross, T.M. Laue, A. Guha, S.V. Thiruvikraman, T.C. Lin, W.H. Konigsberg, Y. Nemerson, Tissue factor and its extracellular soluble domain: The relationship between intermolecular association with factor VIIa and enzymatic activity of the complex, Biochemistry 31 (1992) 3998-4003.
25. B. Gao, S. Li, P.E. Thorpe, A simple and rapid method for purifying the extracellular domain of human tissue factor, Thromb. Res. 91 (1998) 249-253.