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Volumn 25, Issue 3, 2002, Pages 400-408

High level expression and secretion of truncated forms of herpes simplex virus type 1 and type 2 glycoprotein D by the methylotrophic yeast Pichia pastoris

Author keywords

[No Author keywords available]

Indexed keywords

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC VECTORS; HERPESVIRUS 1, HUMAN; HERPESVIRUS 2, HUMAN; PEPTIDE FRAGMENTS; PICHIA; RECOMBINANT PROTEINS; SOLUBILITY; VIRAL ENVELOPE PROTEINS;

EID: 0036413612     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00034-7     Document Type: Article
Times cited : (12)

References (55)
  • 2
    • 0028089018 scopus 로고
    • Herpes simplex virus glycoproteins I and E facilitate cell-to-cell spread in vivo and across junctions of cultured cells
    • K.S. Dingwell, C.R. Brunetti, R.L. Hendricks, Q. Tand, A.J. Rainbow, D.C. Johnson, Herpes simplex virus glycoproteins I and E facilitate cell-to-cell spread in vivo and across junctions of cultured cells, J. Virol. 68 (1994) 834-845.
    • (1994) J. Virol. , vol.68 , pp. 834-845
    • Dingwell, K.S.1    Brunetti, C.R.2    Hendricks, R.L.3    Tand, Q.4    Rainbow, A.J.5    Johnson, D.C.6
  • 3
    • 0002109061 scopus 로고
    • J. Bentz (Ed.), CRC Press Inc., Boca Raton, FL
    • P.G. Spear, in: J. Bentz (Ed.), Viral Fusion Mechanisms, CRC Press Inc., Boca Raton, FL, 1993, pp. 201-232.
    • (1993) Viral Fusion Mechanisms , pp. 201-232
    • Spear, P.G.1
  • 4
    • 0028236443 scopus 로고
    • Glycoprotein C independent binding of herpes simplex virus to cells requires cell surface heparan sulfate and glycoprotein B
    • B.C. Herold, R.J. Visalli, N. Susmarski, C.R. Brandt, P.G. Spear, Glycoprotein C independent binding of herpes simplex virus to cells requires cell surface heparan sulfate and glycoprotein B, J. Gen. Virol. 75 (1995) 1211-1222.
    • (1995) J. Gen. Virol. , vol.75 , pp. 1211-1222
    • Herold, B.C.1    Visalli, R.J.2    Susmarski, N.3    Brandt, C.R.4    Spear, P.G.5
  • 5
    • 0029058864 scopus 로고
    • Viral determinants of the variable sensitivity of herpes simplex virus strains to gD mediated interference
    • H.J. Dean, M.S. Warner, S.S. Terhune, R.M. Johnson, P.G. Spear, Viral determinants of the variable sensitivity of herpes simplex virus strains to gD mediated interference, J. Virol. 69 (1995) 5171-5176.
    • (1995) J. Virol. , vol.69 , pp. 5171-5176
    • Dean, H.J.1    Warner, M.S.2    Terhune, S.S.3    Johnson, R.M.4    Spear, P.G.5
  • 6
    • 0025294236 scopus 로고
    • Soluble forms of herpes simplex virus glycoprotein D bind to a limited number of cell surface receptors and inhibit virus entry into cells
    • D.C. Johnson, R.L. Burke, T. Gregory, Soluble forms of herpes simplex virus glycoprotein D bind to a limited number of cell surface receptors and inhibit virus entry into cells, J. Virol. 64 (1990) 2569-2576.
    • (1990) J. Virol. , vol.64 , pp. 2569-2576
    • Johnson, D.C.1    Burke, R.L.2    Gregory, T.3
  • 7
    • 0026795801 scopus 로고
    • Soluble glycoprotein D blocks herpes simplex virus type 1 infection of rat eyes
    • L.B. Martin, P.C. Montgomery, T.C. Holland, Soluble glycopro-tein D blocks herpes simplex virus type 1 infection of rat eyes, J. Virol. 66 (1992) 5183-5189.
    • (1992) J. Virol. , vol.66 , pp. 5183-5189
    • Martin, L.B.1    Montgomery, P.C.2    Holland, T.C.3
  • 8
    • 0028212250 scopus 로고
    • Identification of functional regions of herpes simplex virus glycoprotein D by using linker-insertion mutagenesis
    • H.Y. Chiang, G.H. Cohen, R.J. Eisenberg, Identification of functional regions of herpes simplex virus glycoprotein D by using linker-insertion mutagenesis, J. Virol. 68 (1994) 2529-2543.
    • (1994) J. Virol. , vol.68 , pp. 2529-2543
    • Chiang, H.Y.1    Cohen, G.H.2    Eisenberg, R.J.3
  • 9
    • 0021339953 scopus 로고
    • Localization and synthesis of an antigenic determinant of herpes simplex virus glycoprotein D that stimulates the production of neutralizing antibody
    • G.H. Cohen, B. Dietzschold, M. Ponce de Leon, D. Long, E. Golub, A. Varrichio, L. Pereira, R.J. Eisenberg, Localization and synthesis of an antigenic determinant of herpes simplex virus glycoprotein D that stimulates the production of neutralizing antibody, J. Virol. 49 (1984) 102-108.
    • (1984) J. Virol. , vol.49 , pp. 102-108
    • Cohen, G.H.1    Dietzschold, B.2    Ponce de Leon, M.3    Long, D.4    Golub, E.5    Varrichio, A.6    Pereira, L.7    Eisenberg, R.J.8
  • 10
    • 0027347128 scopus 로고
    • T cell responses to synthetic peptides of herpes simplex virus type 1 glycoprotein D in naturally infected individuals
    • R.A. Damhof, J.W. Drijfhout, A.J. Scheffer, J.B. Wilterdink, G. Welling, S. Welling-Wester, T cell responses to synthetic peptides of herpes simplex virus type 1 glycoprotein D in naturally infected individuals, Arch. Virol. 130 (1993) 187-193.
    • (1993) Arch. Virol. , vol.130 , pp. 187-193
    • Damhof, R.A.1    Drijfhout, J.W.2    Scheffer, A.J.3    Wilterdink, J.B.4    Welling, G.5    Welling-Wester, S.6
  • 12
    • 0026760594 scopus 로고
    • Disulfide bond structure of glycoprotein D of herpes simplex virus types 1 and 2
    • D. Long, W.C. Wilcox, W.R. Abrams, G.H. Cohen, R.J. Eisenberg, Disulfide bond structure of glycoprotein D of herpes simplex virus types 1 and 2, J. Virol. 66 (1992) 6668-6685.
    • (1992) J. Virol. , vol.66 , pp. 6668-6685
    • Long, D.1    Wilcox, W.C.2    Abrams, W.R.3    Cohen, G.H.4    Eisenberg, R.J.5
  • 15
    • 0030007655 scopus 로고    scopus 로고
    • Structure-function analysis of soluble forms of herpes simplex virus glycoprotein D
    • A.V. Nicola, S.H. Willis, N.N. Naidoo, R.J. Eisenberg, G.H. Cohen, Structure-function analysis of soluble forms of herpes simplex virus glycoprotein D, J. Virol. 70 (1996) 3815-3822.
    • (1996) J. Virol. , vol.70 , pp. 3815-3822
    • Nicola, A.V.1    Willis, S.H.2    Naidoo, N.N.3    Eisenberg, R.J.4    Cohen, G.H.5
  • 19
    • 0030298375 scopus 로고    scopus 로고
    • Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    • R.I. Montgomery, M.S. Warner, B.J. Lum, P.G. Spear, Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family, Cell 87 (1996) 427-436.
    • (1996) Cell , vol.87 , pp. 427-436
    • Montgomery, R.I.1    Warner, M.S.2    Lum, B.J.3    Spear, P.G.4
  • 22
    • 0033621651 scopus 로고    scopus 로고
    • Nectin2α and nectin2δ are low efficiency mediators for entry of herpes simplex virus mutants carrying the Leu25Pro substitution in gD
    • M. Lopez, F. Cocchi, L. Menotti, E. Avitabile, P. Dubreuil, G. Campadelli-Fiume, Nectin2α and nectin2δ are low efficiency mediators for entry of herpes simplex virus mutants carrying the Leu25Pro substitution in gD, J. Virol. 74 (2000) 1267-1274.
    • (2000) J. Virol. , vol.74 , pp. 1267-1274
    • Lopez, M.1    Cocchi, F.2    Menotti, L.3    Avitabile, E.4    Dubreuil, P.5    Campadelli-Fiume, G.6
  • 23
    • 0032551272 scopus 로고    scopus 로고
    • A cell surface protein with herpesvirus entry activity (HveB) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus
    • M.S. Warner, R.J. Geraghty, W.M. Martinez, R.I. Montgomery, J.C. Whitbeck, R. Xu, R.J. Eisenberg, G.H. Cohen, P.C. Spear, A cell surface protein with herpesvirus entry activity (HveB) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus, Virology 246 (1998) 179-189.
    • (1998) Virology , vol.246 , pp. 179-189
    • Warner, M.S.1    Geraghty, R.J.2    Martinez, W.M.3    Montgomery, R.I.4    Whitbeck, J.C.5    Xu, R.6    Eisenberg, R.J.7    Cohen, G.H.8    Spear, P.C.9
  • 24
    • 0031797729 scopus 로고    scopus 로고
    • The ectodomain of a novel member of the immunoglobulin superfamily related to the poliovirus receptor has the attributes of a bona fide receptor for herpes simplex virus 1 and 2
    • F. Cocchi, L. Menotti, P. Mirandola, M. Lopez, G. Campadelli-Fiume, The ectodomain of a novel member of the immunoglobulin superfamily related to the poliovirus receptor has the attributes of a bona fide receptor for herpes simplex virus 1 and 2, J. Virol. 72 (1998) 9992-10002.
    • (1998) J. Virol. , vol.72 , pp. 9992-10002
    • Cocchi, F.1    Menotti, L.2    Mirandola, P.3    Lopez, M.4    Campadelli-Fiume, G.5
  • 25
    • 0032486317 scopus 로고    scopus 로고
    • Entry of alphaherpesvirusses mediated by polio virus receptor-related protein 1 and poliovirus receptor
    • R.J. Geraghty, C. Krummenacher, G.H. Cohen, R.J. Eisenberg, P.G. Spear, Entry of alphaherpesvirusses mediated by polio virus receptor-related protein 1 and poliovirus receptor, Science 280 (1998) 1618-1620.
    • (1998) Science , vol.280 , pp. 1618-1620
    • Geraghty, R.J.1    Krummenacher, C.2    Cohen, G.H.3    Eisenberg, R.J.4    Spear, P.G.5
  • 27
    • 0032446355 scopus 로고    scopus 로고
    • The V domain of herpesvirus Ig-like receptor (HIgR) contains a major functional region in herpes simplex virus-1 entry into cells and interacts physically with the viral glycoprotein D
    • F. Cocchi, M. Lopez, L. Menotti, M. Aoubala, P. Dubreuil, G. Campadelli-Fiume, The V domain of herpesvirus Ig-like receptor (HIgR) contains a major functional region in herpes simplex virus-1 entry into cells and interacts physically with the viral glycoprotein D, Proc. Natl. Acad. Sci. USA 95 (1998) 15700-15705.
    • (1998) Proc. Natl. Acad. Sci. USA , vol.95 , pp. 15700-15705
    • Cocchi, F.1    Lopez, M.2    Menotti, L.3    Aoubala, M.4    Dubreuil, P.5    Campadelli-Fiume, G.6
  • 28
    • 0031820802 scopus 로고    scopus 로고
    • Herpes simplex virus glycoprotein D can bind to poliovirus receptor related protein 1 or herpes entry mediator, two structurally unrelated mediators of virus entry
    • C. Krummenacher, A.V. Nicola, J.C. Whitbeck, H. Lou, W. Hou, R.J. Lambris, R.J. Geraghty, P.G. Spear, G.H. Cohen, R.J. Eisenberg, Herpes simplex virus glycoprotein D can bind to poliovirus receptor related protein 1 or herpes entry mediator, two structurally unrelated mediators of virus entry, J. Virol. 72 (1998) 7064-7074.
    • (1998) J. Virol. , vol.72 , pp. 7064-7074
    • Krummenacher, C.1    Nicola, A.V.2    Whitbeck, J.C.3    Lou, H.4    Hou, W.5    Lambris, R.J.6    Geraghty, R.J.7    Spear, P.G.8    Cohen, G.H.9    Eisenberg, R.J.10
  • 29
    • 0031868961 scopus 로고    scopus 로고
    • Functional region IV of glycoprotein D from herpes simplex virus modulates glycoprotein binding to the herpesvirus entry mediator
    • A.H. Rux, S.H. Willis, A.V. Nicola, W. Hou, C. Peng, H. Lou, G.H. Cohen, R.J. Eisenberg, Functional region IV of glycoprotein D from herpes simplex virus modulates glycoprotein binding to the herpesvirus entry mediator, J. Virol. 72 (1998) 7091-7098.
    • (1998) J. Virol. , vol.72 , pp. 7091-7098
    • Rux, A.H.1    Willis, S.H.2    Nicola, A.V.3    Hou, W.4    Peng, C.5    Lou, H.6    Cohen, G.H.7    Eisenberg, R.J.8
  • 31
    • 0029616337 scopus 로고
    • ADP-ribosyl cyclase and CD38 catalyze the synthesis of a calcium-mobilizing metabolite from NADP
    • R. Aarhus, R.M. Graeff, D.M. Dickeys, T.F. Walseth, H.C. Lee, ADP-ribosyl cyclase and CD38 catalyze the synthesis of a calcium-mobilizing metabolite from NADP, J. Biol. Chem. 270 (1995) 30327-30333.
    • (1995) J. Biol. Chem. , vol.270 , pp. 30327-30333
    • Aarhus, R.1    Graeff, R.M.2    Dickeys, D.M.3    Walseth, T.F.4    Lee, H.C.5
  • 32
    • 0033955337 scopus 로고    scopus 로고
    • Heterologous protein expression in the methylotrophic yeast P. pastoris
    • J.L. Cereghino, J.M. Cregg, Heterologous protein expression in the methylotrophic yeast P. pastoris, Microbiol. Rev. 24 (2000) 45-66.
    • (2000) Microbiol. Rev. , vol.24 , pp. 45-66
    • Cereghino, J.L.1    Cregg, J.M.2
  • 34
    • 0029980110 scopus 로고    scopus 로고
    • Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins
    • C.D. Lima, M.G. Klein, I.B. Weinstein, W.A. Hendrickson, Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins, Proc. Natl. Acad. Sci. USA 93 (1996) 5357-5362.
    • (1996) Proc. Natl. Acad. Sci. USA , vol.93 , pp. 5357-5362
    • Lima, C.D.1    Klein, M.G.2    Weinstein, I.B.3    Hendrickson, W.A.4
  • 35
    • 0028774540 scopus 로고
    • Expression of highly disulfide-bonded proteins in P. pastoris
    • C.E. White, N.M. Kempi, E.A. Komives, Expression of highly disulfide-bonded proteins in P. pastoris, Structure 2 (1994) 1003-1005.
    • (1994) Structure , vol.2 , pp. 1003-1005
    • White, C.E.1    Kempi, N.M.2    Komives, E.A.3
  • 36
    • 0032587512 scopus 로고    scopus 로고
    • Production of large quantities of isotopically labeled protein in P. pastoris by fermentation
    • M.J. Wood, E.A. Komives, Production of large quantities of isotopically labeled protein in P. pastoris by fermentation, J. Biomol. N.M.R. 13 (1999) 149-159.
    • (1999) J. Biomol. N.M.R. , vol.13 , pp. 149-159
    • Wood, M.J.1    Komives, E.A.2
  • 37
    • 0031471296 scopus 로고    scopus 로고
    • The production of recombinant dengue virus E protein using Escherichia coli and P. pastoris
    • R.J. Sugrue, T. Cui, Q. Xu, J. Fu, Y.C. Chan, The production of recombinant dengue virus E protein using Escherichia coli and P. pastoris, J. Virol. Methods 69 (1997) 159-169.
    • (1997) J. Virol. Methods , vol.69 , pp. 159-169
    • Sugrue, R.J.1    Cui, T.2    Xu, Q.3    Fu, J.4    Chan, Y.C.5
  • 38
    • 0030591760 scopus 로고    scopus 로고
    • Intracellular production of a major cytomegalovirus antigenic protein in the methylotrophic yeast P. pastoris
    • M.C. Battista, G. Bergamini, F. Campanini, M.P. Landini, A. Ripalti, Intracellular production of a major cytomegalovirus antigenic protein in the methylotrophic yeast P. pastoris, Gene 176 (1996) 197-201.
    • (1996) Gene , vol.176 , pp. 197-201
    • Battista, M.C.1    Bergamini, G.2    Campanini, F.3    Landini, M.P.4    Ripalti, A.5
  • 39
    • 0030843878 scopus 로고    scopus 로고
    • Protection of mice against a lethal influenza challenge by immunization with yeast-derived recombinant influenza neuraminidase
    • W. Martinet, X. Saelens, T. Deroo, S. Neirynck, R. Contreras, W. Min Jou, W. Fiers, Protection of mice against a lethal influenza challenge by immunization with yeast-derived recombinant influenza neuraminidase, Eur. J. Biochem. 247 (1997) 332-338.
    • (1997) Eur. J. Biochem. , vol.247 , pp. 332-338
    • Martinet, W.1    Saelens, X.2    Deroo, T.3    Neirynck, S.4    Contreras, R.5    Min Jou, W.6    Fiers, W.7
  • 40
    • 0030951077 scopus 로고    scopus 로고
    • Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenicity
    • X. Zhu, S. Wu, G.J. Letchworth III, Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenicity, Vaccine 15 (1997) 679-688.
    • (1997) Vaccine , vol.15 , pp. 679-688
    • Zhu, X.1    Wu, S.2    Letchworth G.J. III3
  • 41
    • 0027746358 scopus 로고
    • The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast P. pastoris
    • C.A. Scorer, R.G. Buckholz, J.J. Clare, M.A. Romanos, The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast P. pastoris, Gene 136 (1993) 111-119.
    • (1993) Gene , vol.136 , pp. 111-119
    • Scorer, C.A.1    Buckholz, R.G.2    Clare, J.J.3    Romanos, M.A.4
  • 45
    • 0020077906 scopus 로고
    • Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies
    • L. Pereira, D.V. Dondero, D. Gallo, V. Devlin, J.D. Woodie, Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies, Infect. Immun. 35 (1982) 363-367.
    • (1982) Infect. Immun. , vol.35 , pp. 363-367
    • Pereira, L.1    Dondero, D.V.2    Gallo, D.3    Devlin, V.4    Woodie, J.D.5
  • 47
    • 0022494423 scopus 로고
    • An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization
    • A.C. Minson, T.C. Hodgman, P. Digard, D.C. Hancock, S.E. Bell, E.A. Buckmaster, An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization, J. Gen. Virol. 67 (1986) 1001-1013.
    • (1986) J. Gen. Virol. , vol.67 , pp. 1001-1013
    • Minson, A.C.1    Hodgman, T.C.2    Digard, P.3    Hancock, D.C.4    Bell, S.E.5    Buckmaster, E.A.6
  • 48
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254.
    • (1976) Anal. Biochem. , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 50
    • 0031777344 scopus 로고    scopus 로고
    • Examination of the kinetics of herpes simplex virus glycoprotein D binding to the herpesvirus entry mediator, using surface plasmon resonance
    • S.H. Willis, A.H. Rux, C. Peng, J.C. Whitbeck, A.V. Nicola, H. Lou, W. Hou, L. Salvador, R.J. Eisenberg, G.H. Cohen, Examination of the kinetics of herpes simplex virus glycoprotein D binding to the herpesvirus entry mediator, using surface plasmon resonance, J. Virol. 72 (1998) 5937-5947.
    • (1998) J. Virol. , vol.72 , pp. 5937-5947
    • Willis, S.H.1    Rux, A.H.2    Peng, C.3    Whitbeck, J.C.4    Nicola, A.V.5    Lou, H.6    Hou, W.7    Salvador, L.8    Eisenberg, R.J.9    Cohen, G.H.10
  • 51
    • 0027954404 scopus 로고
    • High level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein D synthesized by baculovirus-infected insect cells
    • W.P. Sisk, J.D. Bradley, R.J. Leipold, A.M. Stoltzfus, M. de Leon, M. Hilf, C. Peng, G.H. Cohen, R.J. Eisenberg, High level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein D synthesized by baculovirus-infected insect cells, J. Virol. 68 (1994) 766-775.
    • (1994) J. Virol. , vol.68 , pp. 766-775
    • Sisk, W.P.1    Bradley, J.D.2    Leipold, R.J.3    Stoltzfus, A.M.4    De Leon, M.5    Hilf, M.6    Peng, C.7    Cohen, G.H.8    Eisenberg, R.J.9
  • 52
    • 0024637162 scopus 로고
    • Size distribution and general structural features of N-linked oligosaccharides from the methylotrophic yeast, P. pastoris
    • L.S. Grinna, J.F. Tschopp, Size distribution and general structural features of N-linked oligosaccharides from the methylotrophic yeast, P. pastoris, Yeast 5 (1989) 107-115.
    • (1989) Yeast , vol.5 , pp. 107-115
    • Grinna, L.S.1    Tschopp, J.F.2
  • 53
    • 0028349040 scopus 로고
    • Expression of seven herpes simplex virus type 1 glycoproteins (gB, gC, gD, gG, gH and gI): Comparative protection against lethal challenge in mice
    • H. Ghiasi, R. Kaiwar, A.B. Nesburn, S. Slanina, S.L. Wechsler, Expression of seven herpes simplex virus type 1 glycoproteins (gB, gC, gD, gG, gH and gI): Comparative protection against lethal challenge in mice, J. Virol. 68 (1994) 2118-2126.
    • (1994) J. Virol. , vol.68 , pp. 2118-2126
    • Ghiasi, H.1    Kaiwar, R.2    Nesburn, A.B.3    Slanina, S.4    Wechsler, S.L.5
  • 55
    • 0030945466 scopus 로고    scopus 로고
    • Antigenic structure of soluble herpes simplex virus glycoprotein D correlates with inhibition of HSV infection
    • A.V. Nicola, C. Peng, H. Lou, G.H. Cohen, R.J. Eisenberg, Antigenic structure of soluble herpes simplex virus glycoprotein D correlates with inhibition of HSV infection, J. Virol. 71 (1997) 2940-2946.
    • (1997) J. Virol. , vol.71 , pp. 2940-2946
    • Nicola, A.V.1    Peng, C.2    Lou, H.3    Cohen, G.H.4    Eisenberg, R.J.5


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