Entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor

Science

Volumn 280, Issue 5369, 1998, Pages 1618-1620

  Geraghty, Robert J ^a   Krummenacher, Claude ^b   Cohen, Gary H ^b   Eisenberg, Roselyn J ^b   Spear, Patricia G ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RECEPTOR PROTEIN; VIRUS ENVELOPE PROTEIN; VIRUS RECEPTOR;

ANIMAL CELL; ARTICLE; BOVINE HERPES VIRUS; CELL FUSION; CELL LINE; CHO CELL; CONTROLLED STUDY; EPITHELIUM CELL; GENE EXPRESSION; HERPES VIRUS; HUMAN; HUMAN CELL; MUCOSA CELL; NERVOUS SYSTEM; NONHUMAN; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; PSEUDORABIES HERPETOVIRUS; VIRION; VIRUS CELL INTERACTION;

ALPHAHERPESVIRINAE; ANIMALS; BASE SEQUENCE; CELL ADHESION MOLECULES; CELLS, CULTURED; CHO CELLS; CRICETINAE; EPITHELIAL CELLS; GENE EXPRESSION; HERPESVIRUS 1, BOVINE; HERPESVIRUS 1, HUMAN; HERPESVIRUS 1, SUID; HERPESVIRUS 2, HUMAN; HUMANS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; NEURONS; POLYMERASE CHAIN REACTION; RECEPTORS, VIRUS; TRANSFECTION; TUMOR CELLS, CULTURED; VIRAL ENVELOPE PROTEINS;

EID: 0032486317     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5369.1618     Document Type: Article

References (39)

1. P. G. Spear, Semin. Virol. 4, 167 (1993); T. C. Mettenleiter, in Viral Vectors (Academic Press, New York, 1995), pp. 367-393.
2. P. G. Spear, Semin. Virol. 4, 167 (1993); T. C. Mettenleiter, in Viral Vectors (Academic Press, New York, 1995), pp. 367-393.
3. G. Campadelli-Fiume, M. Arsenakis, F. Farabegoli, B. Roizman, J. Virol. 62, 159 (1988); E. A. Petrovskis, A. L. Meyer, L. E. Post, ibid., p. 2196; D. C. Johnson and M. W. Ligas, ibid., p. 4605; D. C. Johnson, R. L. Burke, T. Gregory, ibid. 64, 2569 (1990); A. Karger and T. C. Mettenleiter, Virology 194, 654 (1993); C. C. L. Chase, C. Lohoff, G. J. Letchworth III, ibid., p. 365; W. Lee and A. O. Fuller, J. Virol. 67, 5088 (1993).
4. G. Campadelli-Fiume, M. Arsenakis, F. Farabegoli, B. Roizman, J. Virol. 62, 159 (1988); E. A. Petrovskis, A. L. Meyer, L. E. Post, ibid., p. 2196; D. C. Johnson and M. W. Ligas, ibid., p. 4605; D. C. Johnson, R. L. Burke, T. Gregory, ibid. 64, 2569 (1990); A. Karger and T. C. Mettenleiter, Virology 194, 654 (1993); C. C. L. Chase, C. Lohoff, G. J. Letchworth III, ibid., p. 365; W. Lee and A. O. Fuller, J. Virol. 67, 5088 (1993).
5. G. Campadelli-Fiume, M. Arsenakis, F. Farabegoli, B. Roizman, J. Virol. 62, 159 (1988); E. A. Petrovskis, A. L. Meyer, L. E. Post, ibid., p. 2196; D. C. Johnson and M. W. Ligas, ibid., p. 4605; D. C. Johnson, R. L. Burke, T. Gregory, ibid. 64, 2569 (1990); A. Karger and T. C. Mettenleiter, Virology 194, 654 (1993); C. C. L. Chase, C. Lohoff, G. J. Letchworth III, ibid., p. 365; W. Lee and A. O. Fuller, J. Virol. 67, 5088 (1993).
6. G. Campadelli-Fiume, M. Arsenakis, F. Farabegoli, B. Roizman, J. Virol. 62, 159 (1988); E. A. Petrovskis, A. L. Meyer, L. E. Post, ibid., p. 2196; D. C. Johnson and M. W. Ligas, ibid., p. 4605; D. C. Johnson, R. L. Burke, T. Gregory, ibid. 64, 2569 (1990); A. Karger and T. C. Mettenleiter, Virology 194, 654 (1993); C. C. L. Chase, C. Lohoff, G. J. Letchworth III, ibid., p. 365; W. Lee and A. O. Fuller, J. Virol. 67, 5088 (1993).
7. G. Campadelli-Fiume, M. Arsenakis, F. Farabegoli, B. Roizman, J. Virol. 62, 159 (1988); E. A. Petrovskis, A. L. Meyer, L. E. Post, ibid., p. 2196; D. C. Johnson and M. W. Ligas, ibid., p. 4605; D. C. Johnson, R. L. Burke, T. Gregory, ibid. 64, 2569 (1990); A. Karger and T. C. Mettenleiter, Virology 194, 654 (1993); C. C. L. Chase, C. Lohoff, G. J. Letchworth III, ibid., p. 365; W. Lee and A. O. Fuller, J. Virol. 67, 5088 (1993).
8. G. Campadelli-Fiume, M. Arsenakis, F. Farabegoli, B. Roizman, J. Virol. 62, 159 (1988); E. A. Petrovskis, A. L. Meyer, L. E. Post, ibid., p. 2196; D. C. Johnson and M. W. Ligas, ibid., p. 4605; D. C. Johnson, R. L. Burke, T. Gregory, ibid. 64, 2569 (1990); A. Karger and T. C. Mettenleiter, Virology 194, 654 (1993); C. C. L. Chase, C. Lohoff, G. J. Letchworth III, ibid., p. 365; W. Lee and A. O. Fuller, J. Virol. 67, 5088 (1993).
9. G. Campadelli-Fiume, M. Arsenakis, F. Farabegoli, B. Roizman, J. Virol. 62, 159 (1988); E. A. Petrovskis, A. L. Meyer, L. E. Post, ibid., p. 2196; D. C. Johnson and M. W. Ligas, ibid., p. 4605; D. C. Johnson, R. L. Burke, T. Gregory, ibid. 64, 2569 (1990); A. Karger and T. C. Mettenleiter, Virology 194, 654 (1993); C. C. L. Chase, C. Lohoff, G. J. Letchworth III, ibid., p. 365; W. Lee and A. O. Fuller, J. Virol. 67, 5088 (1993).
10. R. I. Montgomery, M. W. Warner, B. J. Lum, P. G. Spear, Cell 87, 427 (1996).
11. J. C. Whitbeck et al., J. Virol. 71, 6083 (1997); A. V. Nicola et al., ibid. 72, 3595 (1998).
12. J. C. Whitbeck et al., J. Virol. 71, 6083 (1997); A. V. Nicola et al., ibid. 72, 3595 (1998).
13. M. W. Warner et al., Virology, in press.
14. F. Eberle et al., Gene 159, 267 (1995).
15. C. L. Mendelsohn, E. Wimmer, V. R. Racaniello, Cell 56, 855 (1989).
16. M. Lopez et al., Gene 155, 261 (1995).
17. M. T. Shieh et al., J. Cell. Biol. 116, 1273 (1992).
18. The 5′ half of the Prr1 cDNA insert in pBG38 consisted of the Hind III to Bst EII fragment of the I.M.A.G.E. Consortium Clone 140737, obtained from a placenta cDNA library (13). The 3′ half of the Prr1 cDNA extended from the Bst EII site to the stop codon and was obtained by two rounds of PCR amplification of a placenta cDNA library (Clonetech) with use of primers int1 (5′-TCCTTCACCGATGGCACTATCC) and 108 (5′-ACACGTACCACTCCTTCTTG) for the first round and primers int1 and 104 (5′-GCTCTAGAGCGGCTACACGTACCACTCCTT) for the second round. The initial thermocycling conditions were 94°C for 1 min, 70°C for 1 min, and 72°C for 1.5 min. After every three cycles, the annealing temperature was decreased 3°C until 55°C was reached, after which cycling was continued to 35 total cycles. Two percent of the first PCR volume was used as template with the second primer set under the cycling conditions. The Prr1 cDNA was ligated into pcDNA3 (Invitrogen) via Hind III and Xba I sites. The Pvr cDNA (α form) was PCR amplified from a HeLa cDNA library (Invitrogen) as described (16), with primers pvr01 (5′-TCTGGAGCTTGAAGAAGTGGG) and pvr07 (5′-CACCTTGTGCCCTCTGTCTG) for the first round and pvr01 plus pvr08 (5′-CCTCTCAGTCCCGACGCTGT) for subsequent rounds of amplification. The Pvr cDNA was inserted into pcDNA3 via the Eco RV site to yield pBG42.16.
19. - virus was propagated on SW78 cells to obtain infectious virus. The description and references for the virus strains used in Fig. 1 were as described (3) except for HSV-1(mP) (26) and HSV-2(G) (27).
20. R. J. Geraghty and P. G. Spear, data not shown.
21. G. G. Lennon, C. Auffray, M. Polymeropoulos, M. B. Soares, Genomics 33, 151 (1996).
22. Nucleotide sequence analysis of pBG38 was performed by the University of Chicago Cancer Research Center DNA Sequencing Facility. The sequence differences were absences of cytosines at base pairs (bp) 582, 597, and 617 [numbered according to sequence published in (8)]. These changes yield a deletion of one amino acid residue and the resulting sequence Glu-Ala-Glu-Tyr-Gln-Glu-Ile-Arg-Asn-Pro-Asn-Gly-Thr-Val (amino acids 192 to 205) instead of Glu-Ala-Arg-Val-Pro-Gly-Asp-Ser-Gly-Thr-Pro-Met-Ala-Pro-Val (amino acids 192 to 206).
23. U. Francke and B. Francke, Somat. Cell Genet. 7, 171 (1981).
24. S. Koike et al., Embo J. 9, 3217 (1990).
25. B. S. Kwon et al., J. Biol. Chem. 272, 14272 (1997).
26. 6 cells by using the RNeasy kit (Qiagen). The 3′ RACE kit (GibcoBRL) was used for RT. PCR amplification of HveC sequences was performed with int1 (10) and 105 (5′-TCAACACCAGCAGGATGCTC) primers and one round of thermocycling conditions (10). The primers int1 and 105 spanned three predicted exons; therefore, amplification of contaminating genomic DNA would result in a band of greater size than the HveC-cDNA-specific (738 bp) band detected. The β-actin control primers and thermocycling conditions have been described (29). The samples were subjected to electrophoresis on a 1% agarose gel and visualized by ethidium bromide staining.
27. H. Hsu et al., J. Biol. Chem. 272, 13471 (1997); S. A. Marsters et al., ibid., p. 14029.
28. H. Hsu et al., J. Biol. Chem. 272, 13471 (1997); S. A. Marsters et al., ibid., p. 14029.
29. 5 plaque-forming units (pfu) of HSV-1(KOS)tk12 per milliliter (5), 30 mM Hepes, DMEM, and 10% fetal calf serum]. The mixtures were incubated at 37°C for 1 hour and then chilled on ice. The cells were chilled at 4°C for 10 min before infection. Protein and virus mixture (100 μl) was added to each well and incubated at 4°C for 1 hour, after which the plates were transferred to 37°C for 6 hours. Cells were lysed by adding to each well 100 μl of culture medium containing 1% NP-40. Fifty microliters of cell lysate was transferred to another 96-well dish, 50 μl of chlorophenol red-P-D-galactopyranoside was added, and the kinetics of β-Gal activity was measured at 570 nm.
30. T. Terry-Allison et al., J. Virol., in press.
31. C. Krummenacher et al., ibid., in press.
32. C. Krummenacher, G. H. Cohen, R. J. Eisenberg, data not shown.
33. N. Babic et al., J. Gen. Virol. 77, 2277 (1996).
34. J. M. Miller, C. A. Whetstone, L. J. Bello, W. C. Lawrence, J. C. Whitbeck, Am. J. Vet. Res. 56, 870 (1995).
35. M. D. Hoggan and B. Roizman, Am. J. Hyg. 70, 208 (1958).
36. J. M. Ejercito, E. Kieff, B. Roizman, J. Gen. Virol. 2, 357 (1968).
37. J. T. Thomas and L. A. Laimins, J. Virol. 72, 1131 (1998).
38. J. C. Willey et al., Am. J. Respir. Cell Mol. Biol. 14, 262 (1986).
39. -, L. Bello for BHV-1v4a, C. Waltenbaugh for use of his spectrometer, N. Susmarski for work with cell lines and virus stocks, W. Hou for technical assistance, and R. Xu for CHO-IEβ8/HveC cells. We also thank R. Montgomery, M. Warner, and P. Speck for helpful discussions, and R. Longnecker and L. Laimins for suggestions on the manuscript. Supported by a grant from the National Institute of Allergy and Infectious Diseases (RO1 Al 36293 to P.G.S.) and by grants from the National Institute of Neurological Disorders and Stroke (NS-36731, NS-30606, and Al-18289 to R.J.E. and G.H.C.). R.J.G. is supported by National Research Service Award F32 Al09471.