Reduction of the rate of false-positive cultures of Mycobacterium tuberculosis in a laboratory with a high culture positivity rate

Clinical Chemistry and Laboratory Medicine

Volumn 40, Issue 9, 2002, Pages 888-892

  Carroll, Nora M ^a   Richardson, Madalene ^a   Engelke, Erica ^a   de Kock, Marianna ^a   Lombard, Carl ^a   van Helden, Paul D ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

Cultures; DNA fingerprinting; IS6110; Mycobacterium tuberculosis; Tuberculosis

Indexed keywords

TUBERCULOSTATIC AGENT;

ADULT; ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CLINICAL PROTOCOL; CONTROLLED STUDY; COST BENEFIT ANALYSIS; DIAGNOSTIC PROCEDURE; DNA FINGERPRINTING; HEALTH CARE PLANNING; HUMAN; LABORATORY DIAGNOSIS; LUNG TUBERCULOSIS; MAJOR CLINICAL STUDY; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; PROSPECTIVE STUDY; RESOURCE MANAGEMENT; RETROSPECTIVE STUDY; SPUTUM ANALYSIS; TREATMENT FAILURE; WORKLOAD; EQUIPMENT; GENETICS; ISOLATION AND PURIFICATION; LABORATORY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; ORGANIZATION AND MANAGEMENT; REPRODUCIBILITY; SPUTUM; STANDARD;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; UNCULTURED ACTINOMYCETE;

ADULT; BACTERIOLOGICAL TECHNIQUES; DNA FINGERPRINTING; EQUIPMENT CONTAMINATION; FALSE POSITIVE REACTIONS; HUMANS; LABORATORIES; MYCOBACTERIUM TUBERCULOSIS; PROSPECTIVE STUDIES; REPRODUCIBILITY OF RESULTS; SPECIMEN HANDLING; SPUTUM; TUBERCULOSIS, PULMONARY;

EID: 0036407651     PISSN: 14346621     EISSN: None     Source Type: Journal    
DOI: 10.1515/CCLM.2002.157     Document Type: Article

References (16)

1. Burman W, Reves R. Review of false-positive cultures for Mycobacterium tuberculosis and recommendations for avoiding unnecessary treatment. Clin Infect Dis 2000; 31:1390-5.
2. Breese P, Burman W, Hildred M, Stone B, Wilson M, Yang Z, et al. The effect of changes in laboratory practices on the rate of false-positive cultures for Mycobacterium tuberculosis. Arch Pathol Lab Med 2001; 125:1213-6.
3. Bauer J, Thomsen V, Poulsen S, Andersen A. False-positive results from cultures of Mycobacterium tuberculosis due to laboratory contamination confirmed by restriction fragment length polymorphism analysis. Int J Tuberc Lung Dis 2001; 5:789-90.
4. Aber VR, Allen BW, Mitchison DA, Ayuma P, Edwards EA, Keyes AB. Quality control in tuberculosis bacteriology. 1. Laboratory studies on isolated positive cultures and the efficiency of direct smear examination. Tubercle 1980; 61:123-33.
5. Benjamin W, Lok K, Harris R, Brook N, Bond L, Mulcahy D, et al. Identification of a contaminating Mycobacterium tuberculosis strain with a transposition of an IS6110 insertion element resulting in an altered spoligotype. J Clin Micro 2001; 37:1092-6.
6. Chang C, Kim H, Son H, Park S, Lee M, Park S, et al. False-positive growth of Mycobacterium tuberculosis attributable to laboratory contamination confirmed by restriction fragment length polymorphism analysis. Int J Tuberc Lung Dis 2001; 5:861-7.
7. Trakas K, Stosor V, Dietrich S, Mosher L, Warren J. Persistent tuberculosis or specimen contamination? Arch Pathol Lab Med 2000; 124:902-3.
8. Ramos M, Soini H, Roscanni G, Jaques M, Villares M, Musser J. Extensive cross contamination of specimens with Mycobacterium tuberculosis in a reference laboratory. J Clin Micro 1999; 37:916-9.
9. Das S, Chan S, Allen B, Mitchison D, Lowrie D. Application of DNA fingerprinting with IS986 to sequential mycobacterial isolates obtained from pulmonary tuberculosis patients in Hong Kong before, during and after short-course chemotherapy. Tuberc Lung Dis 1993; 74:47-51.
10. Mitchison D, Keyes A, Edwards E, Ayuma P, Byfield S, Nunn A. Quality control in tuberculosis bacteriology. 2. The origin of isolated positive cultures from the sputum of patients in four studies of short course chemotherapy in Africa. Tubercule 1980; 61:135-44.
11. Gascoyne-Binzi D, Barlow R, Frothingham R, Robinson G, Collyins, T, Gelletlie R, et al. Rapid identification of laboratory contamination with Mycobacterium tuberculosis using variable number tandem repeat analysis. J Clin Micro 2001; 39:69-74.
12. Van Duin J, Pijnenburg J, van Rijswoud C, De Haas P, Hendriks W, van Soolingen D. Investigation of cross contamination in a Mycobacterium tuberculosis laboratory using IS6110 DNA fingerprinting. Int J Tuberc Lung Dis 1998; 1:425-9.
13. Wurtz R, Demarais P, Trainor W, McAuley J, Kocka F, Mosher L, et al. Specimen contamination in a mycobacteriology laboratory detected by pseudo outbreak of multidrug resistant tuberculosis: analysis by routine epidemiology and confirmation by molecular technique. J Clin Micro 1996; 34:1017-9.
14. Enarson D, Rieder H, Arnadotti T, Trébucq A. Management of tuberculosis: a guide for low income countries, 5th ed. International Union Against Tuberculosis and Lung Disease, 2000 http://www.iuatld.org/assets/images/Management-of-TB.pdf
15. Warren R, Richardson M, van der Spuy G, Victor T, Sampson S, Beyers N, van Helden P. DNA fingerprinting and molecular epidemiology of tuberculosis: use and interpretation in an epidemic setting. Electrophoresis 1999; 20:1807-12.
16. Richardson M, Carroll NM, Engelke E, Van Der Spuy GD, Salker F, Munch Z, et al. Multiple Mycobacterium tuberculosis strains in early cultures from patients in a high-incidence community setting. J Clin Microbiol 2002; 40:2750-4.