Investigation of cross contamination in a Mycobacterium tuberculosis laboratory using IS6110 DNA fingerprinting

International Journal of Tuberculosis and Lung Disease

Volumn 2, Issue 5, 1998, Pages 425-429

  Van Duin, J M ^a,c   Pijnenburg, J E M ^b   Van Rijswoud, C M ^a   De Haas, P E W ^b   Hendriks, W D H ^a   Van Soolingen, D ^b  

^a Regional Microbiological Center   (Netherlands)

^b NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

^c Zuiderziekenhuis   (Netherlands)

Author keywords

DNA fingerprinting; Laboratory cross contamination; Mycobacterium tuberculosis; RFLP typing

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CLINICAL ARTICLE; DNA FINGERPRINTING; GOOD LABORATORY PRACTICE; HUMAN; HUMAN CELL; HUMAN TISSUE; ISOLATION PROCEDURE; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; STRAIN DIFFERENCE;

BACTERIAL TYPING TECHNIQUES; DNA FINGERPRINTING; DNA, BACTERIAL; FALSE POSITIVE REACTIONS; LABORATORIES; MYCOBACTERIUM TUBERCULOSIS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SPECIES SPECIFICITY;

EID: 0031866923     PISSN: 10273719     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Conville P S, Witebsky F G. Inter bottle transfer of mycobacteria by the Bactec 460. Diagn Microbiol Infect Dis 1989; 12: 401-405.
2. Maurer J R, Desmond E P, Lesser M D, Jones, Jr W D. False-positive cultures of Mycobacterium tuberculosis. Chest 1984; 86:3: 439-443.
3. Murray P R. Mycobacterial cross contamination with the modified Bactec 460 TB. Diagn Microbiol Infect Dis 1991; 14: 33-35.
4. Small P M, McCleeny N B, Singh S P, Schoolnik G K, Thompkins L S, Mickelsen P A. Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures. J Clin Microbiol 1993; 31: 1677-1682.
5. Vannier A N, Tarrand J J, Murray P R. Mycobacterial cross contamination during radiometric culturing. J Clin Microbiol 1988; 26: 1867-1868.
6. Bauer F, Thomsen V, Poulsen S, Andersen A B. False-positive results from cultures of Mycobacterium tuberculosis due to laboratory cross-contamination confirmed by restriction fragment length polymorphism. J Clin Microbiol 1997; 35: 988-991.
7. Cave M D, Eisenach K D, Templeton G, et al. Stability of DNA fingerprint pattern produced with IS6110 in strains of Mycobacterium tuberculosis. J Clin Microbiol 1994; 32: 262-266.
8. Chevrel-Dellagi D, Abderrahman A, Haltiti R, Koubaji H, Gicquel B, Dellagi K. Large-scale DNA fingerprinting of Mycobac- terium tuberculosis strains as a tool for epidemiological studies of tuberculosis. J Clin Microbiol 1993; 31: 2446-2450.
9. Genewein A, Telenti A, Bernasconi C, et al. Molecular approach to identifying route of transmission of tuberculosis in the community. Lancet 1993; 342: 841-844.
10. Van Soolingen D, de Haas P E W, Hermans P W M, van Embden J D A. DNA fingerprinting of Mycobacterium tuberculosis. Methods in Enzym 1993; 235: 196-205.
11. Van Soolingen D, Hermans P W M, de Haas P E W, Soll D R, van Embden J D A. The occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains; evaluation of IS-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol 1991; 29: 2578-2586.
12. Van Soolingen D, de Haas P E W, Hermans P W M, Groenen P M A, van Embden J D A. Comparison of various repetitive elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J Clin Microbiol 1993; 31: 1987-1995.
13. Sommers H M, Good R C. Mycobacterium. Manual of Clinical Microbiology. 4th ed. Am Soc for Microb 1985.
14. Kubica G P, Wayne L G. The Mycobacteria: a source book. New York: Marcel Dekker, Inc., 1984.
15. Kubica G P. Differential identification of mycobacteria VII. Key features of identification of clinically significant mycobacteria. Am Rev Respir Dis 1973; 107-109.
16. Virtanen S. A study of nitrate reduction of infection mycobacteria. Acta Tuberc Scand 1960; (Suppl) 48: 119.
17. Runyon E H, Selin M J, Hawes H W. Distinguishing mycobacteria by the niacin test. Am Rev Tuberc 1959; 79: 663-665.
18. Young W D, Jr, et al. Development of paper strip test for the detection of niacin produced by mycobacteria. Appl Microbiol 1970; 20: 939-945.