Specimen contamination in mycobacteriology laboratory detected by pseudo-outbreak of multidrug-resistant tuberculosis: Analysis by routine epidemiology and confirmation by molecular technique

Journal of Clinical Microbiology

Volumn 34, Issue 4, 1996, Pages 1017-1019

  Wurtz, Rebecca ^a,b,e   Demarais, Patricia ^b   Trainor, William ^a   Mcauley, James ^b,c   Kocka, Frank ^a   Mosher, Laura ^d   Dietrich, Steven ^d  

^a MA   (United States)

^b RUSH MEDICAL COLLEGE   (United States)

^c Chicago Department of Health   (United States)

^d MICHIGAN DEPARTMENT OF COMMUNITY HEALTH   (United States)

^e EVANSTON HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CULTURE; CONTAMINATION; DIAGNOSTIC ERROR; HUMAN; LABORATORY; MULTIDRUG RESISTANCE; PRIORITY JOURNAL; SPUTUM ANALYSIS; TUBERCULOSIS;

BACTERIOLOGICAL TECHNIQUES; DISEASE OUTBREAKS; EPIDEMIOLOGIC METHODS; EPIDEMIOLOGY, MOLECULAR; HUMANS; LABORATORIES; MYCOBACTERIUM TUBERCULOSIS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SPUTUM; TUBERCULOSIS, MULTIDRUG-RESISTANT;

EID: 0029985480     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.4.1017-1019.1996     Document Type: Article

References (15)

1. Aber, V. R., B. W. Allen, D. A. Michison, P. Ayuma, E. A. Edwards, and A. B. Keyes. 1980. Quality control in tuberculosis bacteriology: 1. Laboratory stud-ies on isolated positive cultures and the efficiency of direct smear examination. Tubercle 61:123-133.
2. Conville, P. S., and F. G. Witebsky. 1989. Inter-bottle transfer of mycobacteria by the BACTEC 460. Diagn. Microbiol. Infect. Dis. 12:401-405.
3. Fischl, M. A., R. B. Uttamchandani, G. L. Daikos et al. 1992. An outbreak of tuberculosis caused by multiple-drug-resistant tubercle bacilli among patients with HIV infection. Ann. Intern. Med. 117:177-182.
4. Hong Kong Chest Service. 1981. A study of the characteristics and course of sputum smear-negative pulmonary tuberculosis. Tubercle 62:155-167.
5. Horn, D. L., D. Hewlett, W. H. Haas et al. 1994. Superinfection with rifampin-isoniazid-streptomycin-ethambutol (RISE)-resistant tuberculosis in three patients with AIDS: confirmation by polymerase chain reaction fingerprinting Ann. Intern. Med. 121:115-116.
6. MacGregor, R. R., L. W. Clark, and F. Bass. 1975. The significance of isolating low numbers of Mycobacterium tuberculosis in culture of sputum specimens Chest 68:518-523.
7. Mauer, J. R., E. P. Desmond, M. D. Lesser, and W. D. Jones. 1984. False positive cultures of Mycobacterium tuberculosis. Chest 86:439-443.
8. Mitchison, D. A., A. B. Keyes, E. A. Edwards, P. Ayuma, S. P. Byfield, and A. J. Nunn. 1980. Quality control in tuberculosis bacteriology: 2. The origin of isolated positive cultures from the sputum of patients in four studies of short course chemotherapy in Africa. Tubercle 61:135-144.
9. Murray, P. R. 1991. Mycobacterial cross-contamination with the modified BACTEC 460 TB system. Diagn. Microbiol Infect. Dis. 14:33-35.
10. Small, P. M., N. B. McClenny, S. P. Singh, G. K. Schoolnik, L. S. Tompkins, and P. A. Mickelsen. 1993. Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures. J. Clin. Microbiol. 31:1677-1682
11. Small, P. M., R. W. Shafer, P. C. Hopewell et al. 1993. Exogenous reinfection with multidrug-resistant Mycobacterium tuberculosis in patients with advanced HIV infection. N. Engl. J. Med. 328:1137-1144.
12. van Embden, J. D., M. D. Cave, J. T. Crawford et al. 1993 Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J. Clin. Microbiol. 31:406-409.
13. Vannier, A. M., J. J. Tarrand, and P. R. Murray. 1988. Mycobacterial cross contamination during radiometric culturing. J. Clin. Microbiol. 26:1867-1868.
14. Weinstein, R. A., and W. E. Stamm. 1975. Early detection of false-positive acid-fast smears. Lancet ii:173-174.
15. Weltman, A. C., and D. N. Rose. 1994. Tuberculosis susceptibility patterns, predictors of multidrug resistance, and implications for initial therapeutic regimens at a New York City hospital. Arch. Intern. Med. 154:2161-2167.