Toxicity and immunogenicity of purified Haemophilus ducreyi cytolethal distending toxin in a rabbit model

Microbial Pathogenesis

Volumn 33, Issue 2, 2002, Pages 49-62

  Wising, Catharina ^a   Svensson, Liselott A ^a   Ahmed, Hinda J ^a   Sundaeus, Vivianne ^a   Ahlman, Karin ^a   Jonsson, Ing Marie ^a   Mölne, Lena ^a   Lagergård, Teresa ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

CdtA; CdtB; CdtC; Cytolethal distending toxin; Haemophilus ducreyi; Immunogenicity; Toxicity

Indexed keywords

BACTERIAL PROTEIN; CDT APROTEIN; CDTA PROTEIN; CDTB PROTEIN; CDTC PROTEIN; GENE PRODUCT; UNCLASSIFIED DRUG;

ARTICLE; BLOOD VESSEL; CELL CYCLE; CELL CYCLE G2 PHASE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EOSINOPHIL; ERYTHEMA; GEL FILTRATION; GRANULOCYTE; HAEMOPHILUS DUCREYI; HISTOLOGY; IMMUNIZATION; IMMUNOBLOTTING; IMMUNOGENICITY; ION EXCHANGE; MACROPHAGE; MAMMAL; NONHUMAN; PATHOGENICITY; PATHOLOGY; PRIORITY JOURNAL; PROTEIN PURIFICATION; RABBIT; SKIN MANIFESTATION; TOXICITY;

ESCHERICHIA COLI; HAEMOPHILUS; HAEMOPHILUS DUCREYI; MAMMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0036385621     PISSN: 08824010     EISSN: None     Source Type: Journal    
DOI: 10.1006/mpat.2002.0516     Document Type: Article

References (46)

1. Trees DL, Morse SA. Chancroid and Haemophilus ducreyi: an update. Clin Microbiol Rev 1995; 8: 357-75.
2. Lagergard T. Chapter 86. Haemophilus ducreyi. In: Sussman M, ed. Molecular medical microbiology. London: Academic Press, 2001: 1809-24.
3. Ahmed HJ, Svensson LA, Cope LD, et al. Prevalence of cdtABC genes encoding cytolethal distending toxin among Haemophilus ducreyi and Actinobacillus actinomycetemcomitans strains. J Med Microbiol 2001; 50: 860-4.
4. Pickett CL, Whitehouse CA. The cytolethal distending toxin family. Trends Microbiol 1999; 7: 292-7.
5. Johnson WM, Lior H. Response of Chinese hamster ovary cells to a cytolethal distending toxin (CDT) of Escherichia coli and possible misinterpretation as heat-labile (LT) enterotoxin. FEMS Microbiol Lett 1987; 43: 19-23.
6. Pickett CL, Cottle DL, Pesci EC, Bikah G. Cloning, sequencing, and expression of the Escherichia coli cytolethal distending toxin genes. Infect Immun 1994; 62: 1046-51.
7. Scott DA, Kaper JB. Cloning and sequencing of the genes encoding Escherichia coli cytolethal distending toxin. Infect Immun 1994; 62: 244-51.
8. Johnson WP, Lior H. Production of Shiga toxin and a cytolethal distending toxin (CLDT) by serogroups of Shigella ssp. FEMS Microbiol Lett 1987; 48: 235-8.
9. Okuda J, Kurazono H, Takeda Y. Distribution of the cytolethal distending toxin A gene (cdtA) among species of Shigella and Vibrio, and cloning and sequencing of the cdt gene from Shigella dysenteriae. Microb Pathog 1995; 18: 167-72.
10. Johnson WM, Lior H. A new heat-labile cytolethal distending toxin (CLDT) produced by Campylobacter spp. Microb Pathog 1988; 4: 115-26.
11. Pickett CL, Pesci EC, Cottle DL, Russell G, Erdem AN, Zeytin H. Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene. Infect Immun 1996; 64: 2070-8.
12. Mayer MP, Bueno LC, Hansen EJ, DiRienzo JM. Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. Infect Immun 1999; 67: 1227-37.
13. Sugai M, Kawamoto T, Peres SY, et al. The cell cycle-specific growth-inhibitory factor produced by Actino-bacillus actinomycetemcomitans is a cytolethal distending toxin. Infect Immun 1998; 66: 5008-19.
14. Cortes-Bratti X, Chaves-Olarte E, Lagergard T, Thelestam M. The cytolethal distending toxin from the chancroid bacterium Haemophilus ducreyi induces cell-cycle arrest in the G2 phase. J Clin Invest 1999; 103: 107-15.
15. Gelfanova V, Hansen EJ, Spinola SM. Cytolethal distending toxin of Haemophilus ducreyi induces apoptotic death of Jurkat T cells. Infect Immun 1999; 67: 6394-402.
16. Purven M, Lagergard T. Haemophilus ducreyi, a cytotoxin-producing bacterium. Infect Immun 1992; 60: 1156-62.
17. Svensson LA, Tarkowski A, Thelestam M, Lagergard T. The impact of Haemophilus ducreyi cytolethal distending toxin on cells involved in immune response. Microb Pathog 2001; 30: 157-66.
18. Cope LD, Lumbley S, Latimer JL, et al. A diffusible cytotoxin of Haemophilus ducreyi. Proc Natl Acad Sci USA 1997; 94: 4056-61.
19. Shenker BJ, McKay T, Datar S, Miller M, Chowhan R, Demuth D. Actinobacillus actinomycetemcomitans immunosuppressive protein is a member of the family of cytolethal distending toxins capable of causing a G2 arrest in human T cells. J Immunol 1999; 162: 4773-80.
20. Elwell CA, Dreyfus LA. DNase I homologous residues in CdtB are critical for cytolethal distending toxin-mediated cell cycle arrest. Mol Microbiol 2000; 37: 952-63.
21. Lara-Tejero M, Galan JE. A bacterial toxin that controls cell cycle progression as a deoxyribonuclease I-like protein. Science 2000; 290: 354-7.
22. Frisk A, Lebens M, Johansson C, et al. The role of different protein components from the Haemophilus ducreyi cytolethal distending toxin in the generation of cell toxicity. Microb Pathog 2001; 30: 313-24.
23. Lara-Tejero M, Galan JE. CdtA, CdtB, and CdtC form a tripartite complex that is required for cytolethal distending toxin activity. Infect Immun 2001; 69: 4358-65.
24. Lewis DA, Stevens MK, Latimer JL, et al. Characterization of Haemophilus ducreyi cdtA, cdtB, and cdtC mutants in in vitro and in vivo systems. Infect Immun 2001; 69: 5626-34.
25. Shenker BJ, Hoffmaster RH, McKay TL, Demuth DR. Expression of the cytolethal distending toxin (Cdt) operon in Actinobacillus actinomycetemcomitans: evidence that the CdtB protein is responsible for G2 arrest of the cell cycle in human T cells. J Immunol 2000; 165: 2612-8.
26. Saiki K, Konishi K, Gomi T, Nishihara T, Yoshikawa M. Reconstitution and purification of cytolethal distending toxin of Actinobacillus actinomycetemcomitans. Microbiol Immunol 2001; 45: 497-506.
27. Akifusa S, Poole S, Lewthwaite J, Henderson B, Nair SP. Recombinant Actinobacillus actinomycetemcomitans cytolethal distending toxin proteins are required to interact to inhibit human cell cycle progression and to stimulate human leukocyte cytokine synthesis. Infect Immun 2001; 69: 5925-30.
28. Deng K, Latimer JL, Lewis DA, Hansen EJ. Investigation of the interaction among the components of the cytolethal distending toxin of Haemophilus ducreyi. Biochem Biophys Res Commun 2001; 285: 609-15.
29. Mao X, DiRienzo JM. Functional studies of the recombinant subunits of a cytolethal distending holotoxin. Cell Microbiol 2002; 4: 245-55.
30. Stevens MK, Latimer JL, Lumbley SR, et al. Characterization of a Haemophilus ducreyi mutant deficient in expression of cytolethal distending toxin. Infect Immun 1999; 67: 3900-8.
31. Young RS, Fortney KR, Gelfanova V, et al. Expression of cytolethal distending toxin and hemolysin is not required for pustule formation by Haemophilus ducreyi in human volunteers. Infect Immun 2001; 69: 1938-42.
32. Purdy D, Buswell CM, Hodgson AE, McAlpine K, Henderson I, Leach SA. Characterisation of cytolethal distending toxin (CDT) mutants of Campylobacter jejuni. J Med Microbiol 2000; 49: 473-9.
33. Okuda J, Fukumoto M, Takeda Y, Nishibuchi M. Examination of diarrheagenicity of cytolethal distending toxin: suckling mouse response to the products of the cdtABC genes of Shigella dysenteriae. Infect Immun 1997; 65: 428-33.
34. Li L, Sharipo A, Chaves-Olarte E, et al. The Haemophilus ducreyi cytolethal distending toxin activates sensors of DNA damage and repair complexes in proliferating and non- proliferating cells. Cell Microbiol 2002; 4: 87-99.
35. Lagergard T. The role of Haemophilus ducreyi bacteria, cytotoxin, endotoxin and antibodies in animal models for study of chancroid. Microb Pathog 1992; 13: 203-17.
36. Purven M, Frisk A, Lonnroth I, Lagergard T. Purification and identification of Haemophilus ducreyi cytotoxin by use of a neutralizing monoclonal antibody. Infect Immun 1997; 65: 3496-9.
37. Svensson LA, Henning P, Lagergard T. The cytolethal distending toxin of Haemophilus ducreyi inhibits endothelial cell proliferation. Infect Immun 2002; 70: 2665-9.
38. Relyveld EH, Bizzini B, Gupta RK. Rational approaches to reduce adverse reactions in man to vaccines containing tetanus and diphtheria toxoids. Vaccine 1998; 16: 1016-23.
39. Sambrook J, Fritsch E, Maniatis T. Molecular cloning: a laboratory manual, 2nd ed. N.Y.: Cold Spring Harbor Laboratory Press, 1989.
40. Mattsby-Baltzer I, Lindgren K, Lindholm B, Edebo L. Endotoxin shedding by enterobacteria: free and cell-bound endotoxin differ in Limulus activity. Infect Immun 1991; 59: 689-95.
41. De St Groth SF, Scheidegger D. Production of mono-clonal antibodies: strategy and tactics. J Immunol Methods 1980; 35: 1-21.
42. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.
43. Lagergard T, Purven M. Neutralizing antibodies to Haemophilus ducreyi cytotoxin. Infect Immun 1993; 61: 1589-92.
44. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol 1972; 109: 129-35.
45. Sert V, Cans C, Tasca C, et al. The bacterial cytolethal distending toxin (CDT) triggers a G2 cell cycle checkpoint in mammalian cells without preliminary induction of DNA strand breaks. Oncogene 1999; 18: 6296-304.
46. Towbin H, Steahelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamid gel to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4.