Phosphorylation of the Pseudomonas aeruginosa response regulator AlgR is essential for type IV fimbria-mediated twitching motility

Journal of Bacteriology

Volumn 184, Issue 16, 2002, Pages 4544-4554

  Whitchurch, Cynthia B ^a   Erova, Tatiana E ^a   Emery, Jacqui A ^a   Sargent, Jennifer L ^a   Harris, Jonathan M ^a   Semmler, Annalese B T ^a   Young, Michael D ^a   Mattick, John S ^a   Wozniak, Daniel J ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGR PROTEIN; ASPARTIC ACID; GENE PRODUCT; PROTEIN HISTIDINE KINASE; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BIOFILM; BIOGENESIS; BIOSYNTHESIS; CELL MOTILITY; CONTROLLED STUDY; FIMBRIA; GENE EXPRESSION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; PSEUDOMONAS AERUGINOSA; SIGNAL TRANSDUCTION;

ALGINATES; AMINO ACID SEQUENCE; ASPARTIC ACID; BACTERIAL PROTEINS; BIOFILMS; ELECTROCHEMISTRY; FIMBRIAE, BACTERIAL; GLUCURONIC ACID; HEXURONIC ACIDS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; PHOSPHORYLATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS AERUGINOSA; TRANS-ACTIVATORS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0036334570     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.16.4544-4554.2002     Document Type: Article

References (46)

1. Fimbrial biogenesis genes of Pseudomonas aeruginosa: pilW and pilX increase the similarity of type 4 fimbriae to the GSP protein-secretion systems and pilY1 encodes a gonococcal PilC homologue
2. Identification of a gene, pilV, required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa whose product possesses a prepilin-like leader sequence
3. Genes involved in the biogenesis and function of type-4 fimbriae in Pseudomonas aeruginosa
4. Identification and characterization of AlgZ, an AlgT-dependent DNA binding protein required for Pseudomonas aeruginosa algD transcription
5. Dual function of PilS during transcriptional activation of the Pseudomonas aeruginosa pilin subunit gene
6. Trail following by gliding bacteria
7. The microcolony mode of growth in vivo - An ecological perspective
8. The involvement of cell-to-cell signals in the development of a bacterial biofilm
9. In vitro phosphorylation of AlgR, a regulator of mucoidy in Pseudomonas aentginosa, by histidine protein kinase and effects of small phospho-donor molecules
10. Enzyme-linked immunosorbent assay. Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes
11. Enhancer-like activity of AlgR1-binding site in alginate gene activation: Positional, orientational, and sequence specificity
12. Microbial pathogenesis in cystic fibrosis: Mucoid Pseudomonas aeruginosa and Burkholderia cepacia
13. Protein modelling for all
14. SWISS-MODEL and the Swiss-Pdb-Viewer: An environment for comparative protein modeling
15. PilS and PilR, a two-component transcriptional regulatory system controlling transcription of type 4 fimbriae in Pseudomonas aentginosa
16. Classical electrostatics in biology and chemistry
17. Identification of Pseudomonas aeruginosa genes required for epithelial cell injury
18. Purification of the regulatory protein AlgR1 and its binding in the far upstream region of the algD promoter in Pseudomonas aenuginosa
19. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
20. Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosa
21. Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients
22. The molecular genetics of type-4 fimbriae in Pseudomonas aeruginosa - A review
23. Electrostatic complementarity at protein/protein interfaces
24. Pilus retraction powers bacterial twitching motility
25. AlgR-binding sites within the algD promoter make up a set of inverted repeats separated by a large intervening segment of DNA
26. Versatile gene cassette plasmids to facilitate the construction of generalized and specialized cloning vectors
27. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons
28. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple convergent signalling pathways: A genetic analysis
29. Flagellar and twitching motility are necessarry for Pseudomonas aeruginosa biofilm development
30. Pseudomonas aeruginosa: A key problem in cystic fibrosis
31. Stereochemical criteria for polypeptide and protein chain conformations. II. Allowed conformations for a pair of peptide units
32. Phosphorylation site of NtrC, a protein phosphatase whose covalent intermediate activates transcription
33. Identification of the site of phosphorylation of the chemotaxis response regulator protein, CheY
34. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a protable ori T and the counter-selectable Bacillus subtilis sacB marker
35. A re-examination of twitching motility in Pseudomonas aeruginosa
36. Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms
37. Direct observation of extension and retraction of type IV pili
38. Electrophoretic transfer of proteins from polyacrylamide gels in nitrocellulose sheets: Procedure and some applications
39. Production of single-stranded plasmid DNA
40. New pUC-derived cloning vectors with different selectable markers and DNA replication origins
41. Construction of improved vectors for protein production in Pseudomonas aeruginosa
42. Functional expression of heterologous type 4 fimbriae in Pseudomonas aeruginosa
43. The alginate regulator AlgR and an associated sensor FimS are required for twitching motility in Pseudomonas aeruginosa
44. Transcriptional analysis of the Pseudomonas aeruginosa genes algR, algB and algD reveals a hierarchy of alginate gene expression which is modulated by algT