Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli

EMBO Journal

Volumn 15, Issue 18, 1996, Pages 4798-4805

  Blount, Paul ^a   Sukharev, Sergei I ^a   Moe, Paul C ^a   Schroeder, Matthew J ^a   Guy, H Robert ^b   Kung, Ching ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Mechanosensation; Mechanosensitive channel; MscL; Osmotic forces; Stretch activated channel

Indexed keywords

MEMBRANE PROTEIN; PROTEIN SUBUNIT;

ARTICLE; CLONING; ESCHERICHIA COLI; MEMBRANE CHANNEL; MEMBRANE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ISOLATION;

EID: 0029813032     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00860.x     Document Type: Article

References (53)

1. Armstrong, J.B., Adler, J. and Dahl, M.M. (1967) Nonchemotactic mutants of Escherichia coli. J. Bacteriol., 93, 390-398.
2. Ausubel, F.M., Brent, R.E., Kingston, R.E., Moore, D.D., Seidman, F.G., Smith, J.A. and Struhl, K. (1995) Current Protocols in Molecular Biology. Greene Pub. Associates and Wiley-Interscience, New York.
3. Berrier, C., Coulombe, A., Houssin, C. and Ghazi, A. (1989) A patch-clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes. Pressure-activated channels are localized in the inner membrane. FEBS Lett., 259, 27-32.
4. Berrier, C., Coulombe, A., Szabo, I., Zoratti, M. and Ghazi, A. (1992) Gadolinium ion inhibits loss of metabolites induced by osmotic shock and large stretch-activated channels in bacteria. Eur. J. Biochem., 206, 559-565.
5. Blount, P. and Merlie, J.P. (1990) Mutational analysis of muscle nicotinic acetylcholine receptor subunit assembly. J. Cell Biol., 111, 2613-2622.
6. Boyd, D., Manoil, C. and Beckwith, J. (1987) Determinants of membrane protein topology. Proc. Natl Acad. Sci. USA, 84, 8525-8529.
7. Brickman, E. and Beckwith, J. (1975) Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and φ80 transducing phages. J. Mol. Biol., 96, 307-316.
8. Burke, D.T. and Olson, M.V. (1986) Oligodeoxynucleotide-directed mutagenesis of Escherichia coli and yeast by simple cotransformation of the primer and template. DNA, 5, 325-332.
9. Canessa, C.M., Horisberger, J.D. and Rossier, B.C. (1993) Epithelial sodium channel related to proteins involved in neurodegeneration. Nature, 361, 467-470.
10. Canessa, C.M., Merillat, A.M. and Rossier, B.C. (1994) Membrane topology of the epithelial sodium channel in intact cells. Am. J. Physiol., 267, C1682-C1690.
11. Catterall, W.A. (1995) Structure and function of voltage-gated ion channels. Annu. Rev. Biochem., 64, 493-531.
12. Chumakov, K.M., Rozanov, M.N. and Agol, V.I. (1982) An RNA-dependent nucleoside triphosphate hydrolase from Krebs-II ascites tumor cells. Detection and preliminary characterization. Eur. J. Biochem., 127, 309-314.
13. Cui, C., Smith, D.O. and Adler, J. (1995) Characterization of mechanosensitive channels in Escherichia coli cytoplasmic membrane by whole-cell patch clamp recording. J. Membr. Biol., 144, 31-42.
14. Delcour, A.H., Martinac, B., Adler, J. and Kung, C. (1989) Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels. Biophys. J., 56, 631-636.
15. Derman, A.I. and Beckwith, J. (1991). Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm. J. Bacteriol., 173, 7719-7722.
16. Driscoll, M. and Chalfie, M. (1991) The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration. Nature, 349, 588-593.
17. Driscoll, M. and Chalfie, M. (1992) Developmental and abnormal cell death in C. elegans. Trends Neurosci., 15, 15-19.
18. Ehrmann, M., Boyd, D., and Beckwith, J. (1990) Genetic analysis of membrane protein topology by a sandwich gene fusion approach. Proc. Natl Acad. Sci. USA, 87, 7574-7578.
19. Fleischmann, R.D. et al. (1995) Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science, 269, 496-512.
20. + channels. In Dawson, D. (ed.), Ion Channels and Genetic Diseases. Rockefeller University Press, New York, pp. 1-16.
21. Guy, H.R. and Seetharamulu, P. (1986) Molecular model of the action potential sodium channel. Proc. Natl Acad. Sci. USA, 83, 508-512.
22. Hamill, O.P. and McBride, D.W., Jr (1994) The cloning of a mechanogated membrane ion channel. Trends Neurosci., 17, 439-43.
23. Hamill, O.P., Marty, A., Neher, E., Sakmann, B. and Sigworth, F.J. (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch., 391, 85-100.
24. Harlow, B. and Lane, D. (1988) Antibodies. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold spring Harbor, NY.
25. Häse, C.C., Le Dain, A.C. and Martinac, B. (1995) Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli. J. Biol. Chem., 270, 18329-18334.
26. Huang, M. and Chalfie, M. (1994) Gene interactions affecting mechanosensory transduction in Caenorhabditis elegans. Nature, 367, 467-470.
27. Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J. (1990) PCR Protocols. A Guide to Methods and Applications. Academic Press, Inc., San Diego, CA.
28. Jan, L.Y. and Jan, Y.N. (1994) Potassium channels and their evolving gates. Nature, 371, 119-122.
29. Jentsch, T.J. (1994) Trinity of cation channels. Nature, 367, 412-413.
30. Lingueglia, E., Champigny, G., Lazdunski, M. and Barbry, P. (1995) Cloning of the amiloride-sensitive FMRFamide peptide-gated sodium channel. Nature, 378, 730-733.
31. Lu, Q. and Miller, C. (1995) Silver as a probe of pore-forming residues in a potassium channel. Science, 268, 304-307.
32. Manoil, C. and Beckwith, J. (1986) A genetic approach to analyzing membrane protein topology. Science, 233, 1403-1408.
33. Martinac, B. (1993) Mechanosensitive channels: biophysics and physiology. In Jackson, M.B. (ed.), Thermodynamics of Membrane Receptors and Channels. CRC press, Boca Raton, FL, pp. 327-352.
34. Martinac, B., Buechner, M., Delcour, A.H., Adler, J. and Kung, C. (1987) Pressure-sensitive ion channel in Escherichia coli. Proc. Natl Acad. Sci. USA, 84, 2297-2301.
35. Martinac, B., Adler, J. and Kung, C. (1990) Mechanosensitive ion channels of E. coli activated by amphipaths. Nature, 348, 261-263.
36. Matsushita, O., Jung, C.M. and Okabe, A. (1995) Identification of the gene encoding a mechanosensitive channel MscL homologue in Clostridium perfringens. Gene, 165, 147-148.
37. Milkman, R. (1994) An Escherichia coli homologue of eukuryotic potassium channel proteins. Proc. Natl Acad. Sci. USA, 91, 3510-3514.
38. Munro, A.W., Ritchie, G.Y., Lamb, A.J., Douglas, R.M. and Booth, I.R. (1991) The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli. Mol. Microbiol., 5, 607-616.
39. Parra-Lopez, C., Lin, R., Aspedon, A. and Groisman, E.A. (1994) A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium. EMBO J., 13, 3964-3972.
40. + channel. J. Biol. Chem., 269, 12981-12986.
41. Ruthe, H.J. and Adler, J. (1985) Fusion of bacterial spheroplasts by electric fields. Biochim. Biophys. Acta, 819, 105-113.
42. Sachs, F. (1992) Stretch-sensitive ion channels: an update. In Corey, D.P. and Roper, S.D. (eds), Sensory Transduction. Rockefeller University Press, New York, pp. 242-260.
43. Schlosser, A., Hamann, A., Bossemeyer, D., Schneider, E. and Bakker, E.P. (1993) Mol. Microbiol., 9, 533-43.
44. Schnaitman, C.A. (1970) Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J. Bacteriol., 104, 890-901.
45. Schrempf, H., Schmidt, O., Kummerlen, R., Hinnah, S., Muller, D., Betzler, M., Steinkamp, T. and Wagner, R. (1995) A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans. EMBO J., 14, 5170-5178.
46. Snyder, P.M., McDonald, F.J., Stokes, J.B. and Welsh, M.J. (1994) Membrane topology of the amiloride-sensitive epithelial sodium channel. J. Biol. Chem., 269, 24379-24383.
47. Sukharev, S.I., Martinac, B., Arshavsky, V.Y. and Kung, C. (1993) Two types of mechanosensitive channels in the Escherichia coli cell envelope: solubilization and functional reconstitution. Biophys. J., 65, 177-183.
48. Sukharev, S.I., Blount, P., Martinac, B., Blattner, F.R. and Kung, C. (1994a) A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature, 368, 265-268.
49. Sukharev, S.I., Martinac, B., Blount, P. and Kung, C. (1994b) Functional reconstitution as an assay for biochemical isolation of channel proteins: Application to the molecular identification of a bacterial mechanosensitive channel. Methods: A Companion to Methods in Enzymology, 6, 51-59.
50. Szabo, I., Petronilli, V. and Zoratti, M. (1992) A patch-clamp study of Bacillus subtilis. Biochim. Biophys. Acta, 1112, 29-38.
51. Szabo, I., Petronilli, V. and Zoratti, M. (1993) A patch-clamp investigation of the Streptococcus fuecalis cell membrane. J. Membr. Biol., 131, 203-218.
52. Zoratti, M. and Petronilli, V. (1988) Ion-conducting channels in a Gram-positive bacterium. FEBS Lett., 240, 105-109.
53. Zoratti, M., Petronilli, V. and Szabo, I. (1990) Stretch-activated composite ion channels in Bacillus subtilis. Biochem. Biophys. Res. Commun., 168, 443-450.