At low water activity α-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents

Biotechnology Letters

Volumn 24, Issue 11, 2002, Pages 867-872

  Eckstein, Marrit ^a   Sesing, Martin ^b   Kragl, Udo ^a   Adlercreutz, Patrick ^c  

^a UNIVERSITY OF ROSTOCK   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

^c LUND UNIVERSITY   (Sweden)

Author keywords

Ionic liquids; Water activity; chymotrypsin

Indexed keywords

BUTANOL; CHYMOTRYPSIN A; N ACETYL LEVO PHENYLALANINE ETHYL ESTER; ORGANIC SOLVENT; UNCLASSIFIED DRUG; WATER;

ARTICLE; CATALYSIS; HYDROLYSIS; PROTEIN DEGRADATION; PROTEIN FUNCTION; TRANSESTERIFICATION;

EID: 0036273739     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1015564608261     Document Type: Article

References (28)

1. On the importance of the support material for enzymic synthesis in organic media. Support effects at controlled water activity
2. Activation of enzymes in organic media at low water activity by polyols and saccharides
3. Enzymatic catalysis and dynamics in low-water environments
4. How polar are room-temperature ionic liquids?
5. Enzymatic oxidation of ethanol in the gaseous phase
6. Effects of acetonitrile-water mixtures on alpha-chymotrypsin catalyzed dipeptide synthesis
7. Hydrophobic, highly conductive ambient-temperature molten salts
8. Polarity study of some 1-alkyl- 3-methylimidazolium ambient temperature ionic liquids with the solvatochromic dye, Nile red
9. Enzymatic catalysis of formation of Z-aspartame in ionic liquid - An alternative to enzymatic catalysis in organic solvents
10. Thermodynamic predictions for biocatalysis in non-conventional media: Theory, tests and recommendations for experimental design and analysis
11. Enzyme catalysed synthesis in ambient temperature ionic liquids
12. Biocatalysis in ionic liquids: Markedly enhanced enantioselectivity of lipase
13. Improving enzymes by using them in organic solvents
14. Enzyme catalysis in the presence of ionic liquids
15. α-chymotrypsin catalysis in imidazolium-based ionic liquids
16. Lipase catalysed reactions in ionic liquids
17. Stabilization of α-chymotrypsin by ionic liquids in transesterification reactions
18. Over-stabilization of Candida antarctica lipase B by ionic liquids in ester synthesis
19. Biochemical reactions in supercritical fluids
20. Improved preparation and use of room-temperature ionic liquids in lipase-catalyzed enantio- and regioselective acylations
21. Catalyze gas-phase reactions with enzymes
22. How do organic solvents affect peroxidase structure and function?
23. The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents
24. Enzyme catalysis in ionic liquids: Lipase catalysed resolution of 1-phenylethanol with improved enantioselectivity
25. Reaction rate with suspended lipase catalyst shows similar dependence on water activity in different organic solvents
26. Ionic liquids - New 'solutions' for transition metal catalysis
27. The effect of water on enzyme action inorganic media