Stabilization of α-chymotrypsin by ionic liquids in transesterification reactions

Biotechnology and Bioengineering

Volumn 75, Issue 5, 2001, Pages 563-569

  Lozano, Pedro ^a   De Diego, Teresa ^a   Guegan, Jean Paul ^b   Vaultier, Michel ^b   Iborra, José L ^a  

^a UNIVERSITY OF MURCIA   (Spain)

^b UNIVERSITÉ DE RENNES 1   (France)

Author keywords

chymotrypsin; Enzyme stability; Imidazolium salts; Ionic liquids; Transesterification

Indexed keywords

ESTERIFICATION; NEGATIVE IONS; POSITIVE IONS; RATE CONSTANTS;

BIOCATALYSIS;

ENZYMES;

1 BUTYL 3 METHYLIMIDAZOLIUM HEXAFLUOROPHOSPHATE; AMMONIUM DERIVATIVE; ANION; BIS (TRIFLUOROMETHYL)SULFONILAMIDE; CATION; CHYMOTRYPSIN A; IMIDAZOLE DERIVATIVE; PROPANOL; SULFANILAMIDE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BIOCATALYST; CHEMICAL REACTION KINETICS; ENZYME STABILITY; ESTERIFICATION; FLUORINATION; HALF LIFE TIME; INCUBATION TIME; LIQUID; REACTION ANALYSIS; SYNTHESIS; WATER CONTENT;

1-PROPANOL; ANIMALS; CATALYSIS; CATTLE; CHYMOTRYPSIN; ENZYME STABILITY; ESTERIFICATION; IONS; SOLUTIONS; TYROSINE;

EID: 0035814363     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10089     Document Type: Article

References (30)

1. How polar are room-temperature ionic liquids?
2. Examination of ionic liquids and their interaction with molecules, when used stationary phases in gas chromatography
3. Enhancement of immobilized protease catalyzed dipeptide synthesis by the presence of insoluble protonated nucleophile
4. Hydrophobic, highly conductive ambient temperatures molten salts
5. 2
6. Polarity study of some 1-alkyl-3-methylimidazolium ambient-temperature ionic liquids eith the solvatochromic dye, Nile red
7. Room-temperature replacements for organic solvents in multiphase bioprocess operations
8. Ionic liquids. Green solvents for the future
9. Enzymatic catalysis of formation of Z-aspartame in ionic liquid. An alternative to enzymatic catalysis in organic solvents
10. Designer solvents. Ionic liquids may boost clean technology development
11. News horizons for ionic liquids
12. Biologically active peptides and enzymatic approaches to their production
13. Thermodynamics predictions for biocatalysis in nonconventional media: Theory, test, and recommendations for experimental design and analysis
14. The phase behaviour of 1-alkyl-3-methylimidazolium tetrafluorborates, ionic liquids and ionic liquid crystals
15. Room-temperature ionic liquids as novel media for "clean" liquid-liquid extraction
16. Effects of water content on the enantioselectivity of α-chymotrypsin catalysis in organic media
17. Denaturation capacity: A new criterion for selection of organic solvents as reaction media in biocatalysis
18. Enzymatic reaction in aqueous-organic media. VI. Peptide synthesis by α-chymotrypsin in hydrophillic organic solvents
19. Kinetic analysis of deactivation of immobilized α-chymotrypsin by water-miscible organic solvent in kyotorphin synthesis
20. Designing enzymatic kyotorphin synthesis in organic media with low water content
21. Effect of polyols on α-chymotrypsin thermostability: A mechanistic analysis of the enzyme stabilization
22. Hydrophobicity and water activity relationships of water-miscible aprotic solvents on kyotorphin synthesis catalyzed by α-chymotrypsin
23. Dynamic structure/function relationships in the α-chymotrypsin deactivation process by heat and pH
24. Lipase-catalyzed reactions in ionic liquids
25. Transesterification catalized by polystyrene-supported chymotrypsin in toluene: The effect of neutralization of basic or acidic groups attaching to polystyrene resins
26. The use of new inoic liquids in two-phase catalytic hydrogenation reaction by rhodium complexes
27. Room-temperature molten salts based on quaternary ammonium ion
28. Ionic liquids - New solutions for transition metal catalysis
29. Room-temperature ionic liquids. Solvents for synthesis and catalysis
30. Air and stable 1-ethyl-3-methylimidazolium based ionic liquids