Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay

Planta Medica

Volumn 68, Issue 5, 2002, Pages 445-448

  Bruggisser, Regina ^a   Von Daeniken, Katrin ^a   Jundt, Gernot ^b   Schaffner, Willi ^a   Tullberg Reinert, Heide ^b  

^a UNIVERSITY OF BASEL   (Switzerland)

^b UNIVERSITY HOSPITAL BASEL   (Switzerland)

Author keywords

Antioxidants; MTT tetrazolium assay; Phytoestrogens; Plant extracts

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ACETYLCYSTEINE; ALPHA TOCOPHEROL; ANTIOXIDANT; ASCORBIC ACID; CIMICIFUGA RACEMOSA EXTRACT; CRYSTAL VIOLET; DYE; FORMAZAN; HYPERICUM PERFORATUM EXTRACT; KAEMPFEROL; NATURAL PRODUCT; PHYTOESTROGEN; PLANT EXTRACT; RESVERATROL;

ARTICLE; BREAST CANCER; CANCER CELL; CELL COUNT; CELL FREE SYSTEM; CELL PROLIFERATION; CELL VIABILITY; CHEMICAL INTERACTION; CONTROLLED STUDY; DRUG EFFECT; HUMAN; HUMAN CELL; NITROBLUE TETRAZOLIUM TEST; NONHUMAN; REDUCTION; SCREENING TEST;

ACETYLCYSTEINE; ANTIOXIDANTS; ASCORBIC ACID; BREAST NEOPLASMS; CELL DIVISION; CELL SURVIVAL; DRUG INTERACTIONS; ESTROGENS, NON-STEROIDAL; FLAVONOIDS; HUMANS; ISOFLAVONES; KAEMPFEROLS; PHYTOESTROGENS; PLANT EXTRACTS; PLANT PREPARATIONS; PLANTS; QUERCETIN; STILBENES; TETRAZOLIUM SALTS; THIAZOLES; TUMOR CELLS, CULTURED; VITAMIN E;

CIMICIFUGA; CIMICIFUGA RACEMOSA; HYPERICUM; HYPERICUM PERFORATUM; VIOLA (ANGIOSPERM);

EID: 0036273674     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2002-32073     Document Type: Article

References (15)

1. Studies on succinate tetrazolium reductase system III. Points of coupling of four different tetrazolium salts
2. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
3. Evaluation of tetrazolium-based semi-automated colorimetric assay: Assessment of chemosensitivity testing
4. The MTT assay underestimates the growth inhibitory effects of interferons
5. Use of the tetrazolium assay in measuring the response of human tumor cells to ionizing radiation
6. Validation of the MTT dye assay for enumeration of cells in proliferative and antiproliferative assays
7. Tetrazolium salts and formazans
8. Reduction of tetrazolium salt and superoxide generation in human tumor cells (HeLa)
9. Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction
10. Determination of cell number in monolayer cultures
11. Quantification of cells cultured on 96-well plates
12. Antioxidant compounds interfere with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxity assay
13. Flavonoids as peroxinitrite scavengers: The role of the hydroxyl groups
14. Antioxidant properties of flavonol glycosides from tea
15. The analysis of ginkgo flavonoids