Exon concatenation to increase the efficiency of mutation screening by DGGE

BioTechniques

Volumn 32, Issue 5, 2002, Pages 1064-1070

  Agirre, X ^a   Garcia Delgado M ^a   Calasanz, M J ^a   Larrayoz M J ^a   Novo, F J ^a   Vizmanos, J L ^a  

^a UNIVERSITY OF NAVARRA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; DNA; ELECTROPHORESIS; GENES;

MUTATIONS;

SCREENING;

DNA;

ARTICLE; B CELL LEUKEMIA; CANCER GENETICS; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA DETERMINATION; EXON; GENE MUTATION; GENETIC SCREENING; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; T CELL LEUKEMIA; VALIDATION PROCESS;

EID: 0036253814     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02325rr01     Document Type: Article

References (17)

1. Crystal structure of a p53 tumor suppressor-DNA complex: Understanding tumorigenic mutations
2. 5′ region and exon 7 mutations of the TP53 gene in two cases of B-cell prolymphocytic leukemia
3. Higher sensitivity of denaturing gradient gel electrophoresis than sequencing in the detection of mutations in DNA from tumor samples
4. Single-step DGGE-based mutation scanning of the p53 gene: Application to genetic diagnosis of colorectal cancer
5. p53 gene mutations inside and outside of exons 5-8: The patterns differ in breast and other cancers
6. Comprehensive TP53-denaturing gradient gel electrophoresis mutation detection assay also applicable to archival paraffin-embedded tissue
7. p53 in hematologic malignancies
8. Detection and localization of single base changes by denaturing gradient gel electrophoresis
9. Methods for detection of point mutations: Performance and quality assessment
10. Exhaustive analysis of the p53 gene coding sequence by denaturing gradient gel electrophoresis: Application to the detection of point mutations in acute leukemias
11. The clinical significance of mutations of the P53 tumour supressor gene in haematological malignancies
12. Pooled analysis of p53 mutations in hematological malignancies
13. Detecting single-base mutations
14. Attachment of 40-base pair G+C rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes
15. Structural aspects of the p53 protein in relation to gene evolution
16. DGGE is more sensitive for the detection of somatic point mutations than direct sequencing
17. Exons-introns = lexons: In-frame concatenation of exons by PCR