Proteolytic processing of potyviral proteins and polyprotein processing intermediates in insect and plant cells

Journal of General Virology

Volumn 83, Issue 5, 2002, Pages 1211-1221

  Merits, Andres ^a   Rajamäki, Minna Liisa ^a   Lindholm, Päivi ^a   Runeberg Roos, Pia ^a   Kekarainen, Tuija ^a   Puustinen, Pietri ^a   Mäkeläinen, Katri ^a   Valkonen, Jari P T ^a   Saarma, Mart ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

MUTANT PROTEIN; POLYPROTEIN; PROTEINASE; RNA HELICASE; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CATALYSIS; CELL MEMBRANE; CELL TYPE; CONTROLLED STUDY; ENZYME ACTIVE SITE; GENE DELETION; GENE MUTATION; GENETIC CODE; INSECT CELL; NONHUMAN; PLANT CELL; POTYVIRUS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PROCESSING; VIRUS INFECTIVITY; VIRUS RECOMBINANT; VIRUS REPLICATION;

ANIMALIA; INSECTA; POTATO VIRUS A; POTYVIRUS; SOLANUM TUBEROSUM; UNIDENTIFIED BACULOVIRUS;

EID: 0036252665     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-83-5-1211     Document Type: Article

References (51)

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2. Development and evaluation of ELISA assays incorporating monoclonal antibodies for detection of potato A potyvirus
3. Small nuclear inclusion protein encoded by plant potyvirus genome is a protease
4. Processing of the tobacco etch virus 49K proteinase requires autoproteolysis
5. Mutational analysis of tobacco etch virus polyprotein processing: Cis and trans proteolytic activities of polyproteins containing the 49-kilodalton proteinase
6. A second proteinase encoded by a plant potyvirus genome
7. Internal cleavage and trans-proteolytic activities of the VPg-proteinase (NIa) of tobacco etch potyvirus in vivo
8. Genetic evidence for an essential role for potyvirus CI protein in cell-to-cell movement
9. RNA binding activity of NIa proteinase of tobacco etch potyvirus
10. Functional analysis of the interaction between VPg-proteinase (NIa) and RNA polymerase (NIb) of tobacco etch potyvirus, using conditional and suppressor mutants
11. In vitro interactions between a potyvirus-encoded, genome-linked protein and RNA-dependent RNA polymerase
12. RNA helicase activity of the plum pox potyvirus CI protein expressed in Escherichia coli. Mapping of an RNA binding domain
13. Mutational analysis of plum pox potyvirus polyprotein processing by the NIa protease in Escherichia coli
14. Proteolytic processing of the plum pox potyvirus polyprotein by the NIa protease at novel cleavage site
15. Towards a protein interaction map of potyviruses: Protein interaction matrixes of two potyviruses based on the yeast two-hybrid system
16. The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants
17. Recessive and dominant resistance interfere with the vascular transport of Potato virus A in diploid potatoes
18. Changing patterns of localization of the tobacco mosaic virus movement protein and replicase to the endoplasmatic reticulum and microtubules during infection
19. RNA polymerase activity catalysed by potyvirus encoded RNA-dependent-RNA-polymerase
20. A potyvirus polymerase interacts with the viral coat protein and VPg in yeast cells
21. Expression, purification, and identification of a novel self-cleavage site of the NIa C-terminal 27 kDa protease of turnip mosaic potyvirus C5
22. Effects of internal cleavages and mutations in the C-terminal region of NIa proteinase of turnip mosaic potyvirus on the catalytic activity
23. Mutational analysis of the tobacco vein mottling virus genome
24. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
25. Polypeptide requirements for assembly of functional Sindbis virus replication complex: A model for temporal regulation of minus and plus-strand RNA synthesis
26. Functions of the tobacco etch virus RNA polymerase (NIb): Subcellular transport and protein-protein interactions with VPg/proteinase (NIa)
27. Synthesis of potyviral RNA and proteins in tobacco mesophyll protoplasts inoculated by electroporation
28. Properties of the active plum pox potyvirus RNA polymerase complex in defined glycerol gradient fractions
29. VPg, coat protein and five non-structural proteins of potato A potyvirus bind RNA in a sequence unspecific manner
30. Biochemical and genetic evidence for interactions between potato A potyvirus-encoded proteins P1 and P3 and proteins of the putative replication complex
31. The VPg of tobacco etch virus RNA is the 49 kDa proteinase or the N-terminal 24 kDa part of the proteinase
32. Replacement of the tyrosine residue that links a potyviral VPg to the viral RNA is lethal
33. Identification of the genome-linked protein in virions of potato virus A, with comparison to other members in genus Potyvirus
34. Complete replication in vitro of tobacco mosaic virus RNA by a template-dependent, membrane-bound RNA polymerase
35. Expression and purification of a recombinant tobacco etch virus NIa proteinase: Biochemical analysis of the full-length and a naturally occurring truncated proteinase form
36. Semliki Forest virus-specific non-structural protein nsP3 is a phosphoprotein
37. The nucleotide sequence of potato virus A genomic RNA and its sequence similarities with other potyviruses
38. Infectious in vitro transcripts from cloned cDNA of the potato A potyvirus
39. The 6K2 protein and the VPg of Potato virus A are determinants of systemic infection in Nicandra physaloides
40. Biological, serological, and molecular differences among isolates of potato A potyvirus
41. Tobacco mosaic virus infection induces severe morphological changes of endoplasmatic reticulum
42. The tobacco etch potyvirus 6-kilodalton protein is membrane associated and involved in virus replication
43. Highlights and prospects of potyvirus molecular biology
44. Processing of the plum pox virus polyprotein at the P3-6K1 junction is not required for virus viability
45. Ultrastructural and temporal observations of the potyvirus cylindrical inclusions (Cis) shows that CI protein acts transiently in aiding virus movement
46. Two newly detected non-structural proteins in potyvirus-infected cells
47. Analysis of the VPg-proteinase (NIa) encoded by tobacco etch potyvirus: Effects of mutations on subcellular transport, proteolytic processing, and genome amplification
48. Formation of plant RNA virus replication complexes on membranes: Role of an endoplasmatic reticulum-targeted viral protein
49. Regulation of Sindbis virus RNA replication: Uncleaved P123 and nsP2 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis
50. Expression of potyvirus proteins in insect cells infected with recombinant baculovirus
51. The 35-kDa protein from the N-terminus of the potyviral polyprotein functions as a third virus-encoded proteinase