Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity

Virology

Volumn 226, Issue 2, 1996, Pages 183-190

  Kim, Do Hyung ^a   Hwang, Duk Chul ^a   Kang, Byoung Heon ^a   Lew, Jisun ^a   Han, Jisun ^a   Song, Byeong Doo ^b   Choi, Kwan Yong ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b Handong University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; TURNIP YELLOW MOSAIC VIRUS;

EID: 0030589581     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1996.0645     Document Type: Article

References (28)

1. Allaire, M., Chernaia, M. M., Malcolm, B. A., and James, M. N. G. (1994). Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases. Nature 369, 72-76.
2. Allison, R. F., Johnston, R. E., and Dougherty, W. G. (1986). Nucleotide sequence of the coding region of tobacco etch virus genomic RNA: Evidence for the synthesis of a single polyprotein. Virology 154, 9-20.
3. Bazan, J. F., and Fletterick, R. J. (1988). Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: Structural and functional implications. Proc. Natl. Acad. Sci. USA 85, 7872-7876.
4. Carrington, J. C., and Dougherty, W. G. (1987). Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. J Virol. 61, 2540-2548.
5. Carrington, J. C., Cary, S. M., Parks, T. D., and Dougherty, W. G. (1989). A second proteinase encoded by a plant potyvirus genome. EMBO J. 8, 365-370.
6. Creighton, T. E. (1993). In "Proteins, Structures and Molecular Properties," 2nd ed., pp. 7-8. Freeman, New York.
7. Domier, L. L., Franklin, K. M., Shahabuddin, M., Hellmann, G. M., Overmyer, J. H., Hiremath, S. T., Siaw, M. F. E., Lomonossoff, G. P., Shaw, J. G., and Rhoades, R. E. (1986). The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Res. 14, 5417-5430.
8. Dougherty, W. G., and Carrington, J. C. (1988). Expression and function of potyviral gene products. Annu. Rev. Phytopathol. 26, 123-143.
9. Dougherty, W. G., and Parks, T. D. (1991). Post-translational processing of the tobacco etch virus 49-kDa small nuclear inclusion polyprotein: Identification of an internal cleavage site and delimitation of VPg and proteinase domains. Virology 183, 449-456.
10. Dougherty, W. G., Parks, T. D., Cary, S. M., Bazan, J. F., and Fletterick, R. J. (1989). Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase. Virology 172, 302-310.
11. Dougherty, W. G., Parks, T. D., Smith, H. A., and Lindbo, J. A. (1990). Expression of the potyvirus genome: The role of proteolytic processing. In "Viral Genes and Plant Pathogenesis" (Piron, T. P., and Shaw, J. G., Eds.), pp. 124-139. Springer-Verlag, New York.
12. Gauss-Muller, V., Jurgensen, D., and Deutzmann, R. (1991). Autocatalytic cleavage of recombinant 3C proteinase of hepatitis A virus. Virology 182, 861-864.
13. Gorbalenya, A. E., Donchenko, A. P., Blinov, V. M., and Koonin, E. V. (1989). Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. FEBS Lett. 243, 103-114.
14. Guan, K. L., and Dixon, J. E. (1991). Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192, 262-267.
15. Kim, D.-H., Hwang, D. C., Kang, B. H., Lew, J., and Choi, K. Y. (1996). Characterization of the NIa protease from turnip mosaic potyvirus exhibiting a low-temperature optimum catalytic activity. Virology 221, 245-249.
16. Kim, D.-H., Park, Y. S., Kim, S. S., Lew, J., Nam, H. G., and Choi, K. Y. (1995). Expression, purification, and identification of a novel self-cleavage site of the NIa C-terminal 27-kDa protease of turnip mosaic potyvirus C5. Virology 213, 517-525.
17. Kunkel, T. A. (1985). Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82, 488-492.
18. Lain, S., Reichmann, J. L., Mendez, E., and Garcia, J. A. (1988). Nucleotide sequence of the 3′ terminal region of plum pox potyvirus RNA. Virus Res. 10, 325-342.
19. Matthews, D. A., Smith, W. W., Ferre, R. A., Condon, B., Budahazi, G., Sisson, W., Villafranca, J. E., Janson, C. A., McElroy, H. E., Gribskov, C. L., and Worland, S. (1994). Structure of human rhinovirus 3C protease reveals a trypsin-like polypeptide fold, RNA-binding site, and means for cleaving precursor polyprotein. Cell 77, 761-771.
20. Mohr, H., and Schopfer, P. (1995). "Plant Physiology," pp. 261-263. Springer-Verlag, New York.
21. Murphy, J. F., Rhoads, R. E., Hunt, A. G., and Shaw, J. G. (1990). The VPg of tobacco etch virus RNA is the 49-kDa proteinase or the N-terminal 24-kDa part of the proteinase. Virology 178, 285-288.
22. Nicolas, O., and Laliberté, J. F. (1992). The complete nucleotide sequence of turnip mosaic potyvirus RNA. J. Gen. Virol. 73, 2785-2793.
23. Pace, C. N., Vajdos, F., Fee, L., Grimsley, G., and Gray, T. (1995). How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 4, 2411-2423.
24. Parks, T. D., Howard, E. D., Wolpert, T. J., Arp, D. J., and Dougherty, W. G. (1995). Expression and purification of a recombinant tobacco etch virus NIa proteinase: Biochemical analyses of the full-length and a naturally occurring truncated proteinase form. Virology 210, 194-201.
25. Robaglia, C., Durand-Tardif, M., Tronchet, M., Boudazin, G., Astier-Manifacier, S., and Casse-Delbart, F. (1989). Nucleotide sequence of potato virus Y (N strain) genomic RNA. J. Gen. Virol. 70, 935-947.
26. Rose, J. R., Salto, R., and Craik, C. S. (1993). Regulation of autoproteolysis of the HIV-1 and HIV-2 proteinases with engineered amino acid substitutions. J. Biol. Chem. 268, 11939-11945.
27. Schulz, G. E., and Schirmer, R. H., Eds. (1979). "Principles of Protein Structure," pp. 10-14. Springer-Verlag, New York.
28. Verchot, J., Koonin, E. V.and Carrington, J. C. (1991). The 35-kDa protein from the N-terminus of the potyviral polyprotein functions as a third virus-encoded proteinase. Virology 185, 527-535.