Intracellular flow cytometric analysis of primary cultured breast tumor cells

Cancer Investigation

Volumn 20, Issue 3, 2002, Pages 340-347

  Loveday, Ruth L ^a   Speirs, Valerie ^a   Drew, Philip J ^a   Kerin, Michael J ^a   Monson, John R T ^a   Greenman, John ^a  

^a UNIVERSITY OF HULL   (United Kingdom)

Author keywords

Breast; Cytokeratin 19; Intracellular flow cytometry; Primary culture

Indexed keywords

CYTOKERATIN 19; VIMENTIN;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CELL TYPE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MICROSCOPY; PRIORITY JOURNAL; TUMOR BIOPSY;

BREAST NEOPLASMS; DNA, NEOPLASM; FLOW CYTOMETRY; GENE FREQUENCY; HUMANS; KERATINS; QUALITY CONTROL; SPECIMEN HANDLING; TUMOR CELLS, CULTURED;

EID: 0036246735     PISSN: 07357907     EISSN: None     Source Type: Journal    
DOI: 10.1081/CNV-120001179     Document Type: Article

References (36)

1. Comparative study of cytokine gene transcripts in normal and malignant breast tissue. Primary cell cultures derived from the same tissue samples
2. A human cell line from a pleural effusion derived from a breast carcinoma
3. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas
4. A newly established metastatic breast tumor cell line with integrated amplified copies of erbB2 and double minute chromosomes
5. Genetic and epigenetic regulation of human breast cancer progression and metastasis
6. Immortalization in culture: Occurrence at a late stage in the progression of breast cancer
7. Culture systems for study of human mammary epithelial cell proliferation, differentiation and transformation
8. Short-term primary culture of epithelial cells derived from human breast tumours
9. Cholera toxin and analogues of cyclic AMP stimulate the growth of cultured human mammary epithelial cells
10. Epidermal growth factor stimulation of human breast cancer cells in culture
11. Routine culturing of normal, dysplastic and malignant human mammary epithelial cells from small tissue samples
12. Culture of normal and malignant primary human mammary epithelial cells in a physiological manner simulates in vivo growth patterns and allows discrimination of cell type
13. Growth of normal human mammary cells in culture
14. Use of an efficient method for culturing human mammary epithelial cells to study adriamycin sensitivity
15. Serum-free growth of human mammary epithelial cells: Rapid clonal growth in defined medium and extended serial passage with pituitary extract
16. Quantitative variation of proto-oncogene and cytokine gene expression in isolated breast fibroblasts
17. Secretion of a TGF-beta-like growth inhibitor by normal rat mammary epithelial cells in vitro
18. Human breast cancers respond to growth factors in vivo but not in vitro
19. Fibroblasts regulate the migration of MCF7 mammary carcinoma cells in hydrated collagen gel
20. Effects of estrogen, epidermal growth factor, and transforming growth factor-alpha on the growth of human breast epithelial cells in primary culture
21. Comparative study of human and rat mammary tumorigenesis
22. Differential regulation of lymphokine production in mitogen-stimulated murine spleen cells
23. Transformation of human mammary epithelial cells by oncogenic retroviruses
24. Human papilloma virus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements
25. Tumor progression in four mammary epithelial cell lines derived from the same patient
26. Production of and responsiveness to transforming growth factor-β in normal and oncogene-transformed human mammary epithelial cells
27. Human malignant mammary epithelial cell in culture
28. Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: Relation to in vivo phenotypes and influence of medium
29. Patterns of cytokeratin expression by neoplastic and non-neoplastic epithelium
30. Selective cell culture of primary breast carcinomas
31. Microstaging of breast cancer patients using cytokeratin staining of the sentinel lymph node
32. Quantitative polymerase chain reaction for the detection of micrometastases in patients with breast cancer
33. Analysis of sensitivity and specificity of cytokeratin 19 reverse transcriptase-polymerase chain reaction for detection of occult breast cancer in bone marrow and leukapheresis products
34. Cancer invasion and metastasis
35. Differential regulation of breast tumor cell proliferation by stromal fibroblasts of various breast tissue sources