Short term primary culture of epithelial cells derived from human breast tumours

British Journal of Cancer

Volumn 78, Issue 11, 1998, Pages 1421-1429

  Speirs, V ^a   Green, A R ^a   Walton, D S ^a   Kerin, M J ^a   Fox, J N ^a   Carleton, P J ^a   Desai, S B ^b   Atkin, S L ^a  

^a UNIVERSITY OF HULL   (United Kingdom)

^b SCUNTHORPE GENERAL HOSPITAL   (United Kingdom)

Author keywords

Breast; Culture; Epithelial; Tumour

Indexed keywords

CYTOKERATIN; EPITHELIAL MEMBRANE ANTIGEN; ESTROGEN RECEPTOR; TESTOSTERONE 17BETA DEHYDROGENASE;

ARTICLE; BREAST EPITHELIUM; BREAST TUMOR; CANCER CELL CULTURE; CANCER STAGING; CELL GROWTH; CELL SEPARATION; CELL VIABILITY; CENTRIFUGATION; CONTROLLED STUDY; ENZYME ACTIVITY; EPITHELIUM CELL; HISTOLOGY; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; LEIOMYOSARCOMA; PHENOTYPE; PLEURA EFFUSION; PRIORITY JOURNAL;

EID: 0031739920     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/bjc.1998.702     Document Type: Article

References (44)

1. Adams EF, Coldham NG and James VHT (1988a) Steroidal regulation of oestradiol-17β dehydrogenase activity of the human breast cancer cell line MCF-7. J Endocrinol 118: 149-154
2. Adams EF, Newton CJ, Braunsberg H, Shaikh N, Ghilchik MW and James VHT (1988b) Effects of human breast fibroblasts on growth and 17β-estradiol dehydrogenase activity of MCF-7 cells. Breast Cancer Res Treat 11: 165-172
3. Band V, Zajchowski D, Swisshelm D, Trask D, Kulesa V, Cohen C, Conolly J and Sager R (1990) Tumour progression in four mammary epithelial cell lines derived from the same patient. Cancer Res 50: 7351-7357
4. Bartek J, Taylor-Papadimitrou J, Miller N and Millis R (1985) Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours. Int J Cancer 35: 359-364
5. Bergstraesser LM and Weitzman SA (1993) Culture of normal and malignant human mammary epithelial cells in a physiological manner simulates in vivo growth patterns and allows discrimination of cell type. Cancer Res 53: 2644-2654
6. Cailleau R, Young R, Olive M and Reeves WJJ (1974) Breast tumour cell lines from pleural effusions. J Natl Cancer Inst 53: 661-666
7. Castegnetta LA, Granata OM, Taibi G, Casto ML, Comito L, Olivieri G, Di Falco M and Carruba G (1996) 17β-Hydroxysteroid oxidoreductase activity in intact cells significantly differs from classical enzymology analysis. J Endocrinol 150: S73-S78
8. Clarke RB, Howell A, Potten CS and Anderson E (1997) Dissociation between steroid receptor expression and cell proliferation in the human breast. Cancer Res 57: 4987-4991
9. Dairkee SH, Deng G, Stampfer MR, Waldman FM and Smith HS (1997) Partial enzymatic degradation of stroma allows enrichment and expansion of primary breast tumour cells. Cancer Res 57: 1590-1596
10. Emerman JT and Wilkinson DA (1990) Routine culturing of normal, dysplastic and malignant human mammary epithelial cells from small tissue samples. In Vitro Cell Devel Biol 26: 1186-1194
11. Emerman JT, Stingl J, Petersen A, Shpall EJ and Eaves CJ (1996) Selective growth of freshly isolated human breast epithelial cells cultured at low concentrations in the presence or absence of bone marrow cells. Breast Cancer Res Treat 41: 147-159
12. Ethier SP, Mahacek ML, Gullick WJ, Frank TS and Weber BL (1993) Differential isolation of normal luminal mammary epithelial cells and breast cancer cells from primary and metastatic sites. Cancer Res 53: 627-635
13. Green AR, Green VL, White MC and Speirs V (1997) Expression of cytokine messenger RNA in normal and neoplastic human breast tissue: identification of interleukin-8 as a potential regulatory factor in breast tumours. Int J Cancer 72: 937-941
14. Hofland LJ, van der Burg B, van Eijck CHJ, Sprij DM, van Koetsveld PM and Lamberts SWJ (1995) Role of tumour-derived fibroblasts in the growth of primary cultures of human breast cancer cells: effects of epidermal growth factor and the somatostatin analogue octreotide. Int J Cancer 60: 93-99
15. Hsu SM, Raine L and Fanger H (1981) The use of avidin-biotin complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29: 577-580
16. Lippman ME, Dickson RB, Kasid A, Gelman E, Davidson N, McManaway N, Huff K, Bronzert D, Bates S and Knabbe C (1989) Autocrine and paracrine growth regulation of human breast cancer. J Steroid Biochem 24: 147-154
17. McCallum HM and Lowther GW (1996) Long-term culture of primary breast cancer in defined medium. Breast Cancer Res Treat 39: 247-259
18. Meltzer P, Leibovitz A, Dalton W, Villar H, Kute T, Davies J, Nagle R and Trent J (1991) Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. Br J Cancer 63: 727-735
19. Nasir J and Speirs V (1997) Rapid and irreversible loss of estrogen receptor in human osteoblast-like cells following culture in phenol red-tree medium. In Vitro Cell Dev Biol 33: 240-242
20. O'Hare MJ, Ormerod MG, Monaghan P, Lane EB and Gusterson BA (1991) Characterisation in vitro of luminal and myoepithelial cells isolated from the human mammary gland by cell sorting. Differentiation 46: 209-221
21. Ogmundsdottir HM, Petursdottir I, Gudmundsdottir I, Amundsdottir L, Ronnov-Jessen L and Petersen OW (1993) Effects of lymphocytes and fibroblasts on the growth of human mammary carcinoma cells studies in short-term primary cultures. In Vitro Cell Devel Biol 29A: 936-942
22. Osborne CK and Arteaga CL (1990) Autocrine and paracrine growth regulation of breast cancer: clinical implications. Breast Cancer Res Treat 15: 3-11
23. Osborne CK, Hobbs K and Trent JM (1987) Biological differences among MCF-7 human breast cancer cells lines from different laboratories. Breast Cancer Res Treat 9: 111-121
24. Peltoketo H, Isomaa V, Poutanen M and Vihko R (1996) Expression and regulation of 17β-hydroxysteroid dehydrogenase type I. J Endocrinol 150: S21-S30
25. Petersen OW, van Deurs B, Nielsen K, Madsen MW, Laursen I, Balslev I and Briand P (1990) Differential tumorigenicity of two autologous human breast carcinoma cell lines, HMT-390951 and HMT-390958, established in serum-free medium. Cancer Res 50: 1257-1270
26. Pink JJ, Bilimoria MM, Assikis J and Jordan VC (1996) Irreversible loss of oestrogen receptor in T47D breast cancer cells following prolonged oestrogen deprivation. Br J Cancer 74: 1227-1236
27. Poutanen M, Isomaa V, Peltoketo H and Vihko R (1995) Role of 17β-hydroxysteroid dehydrogenase type I in endocrine and intracrine estradiol biosynthesis. J Steroid Biochem Molec Biol 55: 525-532
28. Reed MJ, Singh A, Ghilchik MW, Coldham NG and Purohit A (1991) Regulation of oestradiol 17β-hydroxysteroid dehydrogenase in breast tissues: the role of growth factors. J Steroid Biochem Molec Biol 39: 791-798
29. Sannino P and Shousa S (1994) Demonstration of oestrogen and its receptors in paraffin wax section of breast carcinoma using the monoclonal antibody 1D5 and microwave oven processing. J Clin Pathol 47: 90-92
30. Schoenfield A, Luqmani Y, Sinnett HD, Shousa S and Coombes RC (1996) Keratin 19 mRNA measurement to detect micrometastases in lymph nodes in breast cancer patients. Br J Cancer 74: 1639-1642
31. Schoenfield A, Luqmani Y, Smith D, O'Reilly S, Shousa S, Sinnett SD and Coombes RC (1994) Detection of breast cancer micrometastases in axillary lymph nodes by using polymerase chain reaction. Cancer Res 54: 2896-2990
32. Shearer M, Bartkova J, Bartek J, Berdichevsky F, Barnes D, Millis R and Taylor-Papadimitrou (1992) Studies of clonal cell lines developed from primary breast cancers indicate that the ability to undergo morphogenesis in vitro is lost early in malignancy. Int J Cancer 51: 602-612
33. Soule HD, Vasquez J, Long A, Albert S and Brennan M (1973) A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst 51: 1409-1413
34. Speirs V, Adams EF, Rafferty B and White MC (1993) Interactive effects of interleukin-6, 17β-estradiol and progesterone on growth and 17β-hydroxysteroid activity in human breast carcinoma cells. J Steroid Biochem Mol Biol 46: 11-15
35. Speirs V, Birch MA, Boyle-Walsh E, Green AR, Gallagher JA and White MC (1995) Interleukin 3: a putative protective factor against breast cancer which is secreted by male but not female breast fibroblasts. Int J Cancer 61: 416-419
36. Speirs V, Green AR and White MC (1996a) A comparative study of cytokine gene transcripts in normal and malignant breast tissue and primary cell culture derived from the same tissue samples. Int J Cancer 66: 551-556
37. Speirs V, Green AR and White MC (1996b) Collagenase III: a superior enzyme for complete disaggregation and improved viability of normal and malignant human breast tissue. In Vitro Cell Devel Biol 32: 72-74
38. Stampfer M and Yaswen P (1993) Culture systems for study of human mammary epithelial cell proliferation, differentiation and transformation. Cancer Surveys 18: 7-34
39. Taylor-Papadimitrou J, Stampfer M, Bartek J, Lewis A, Boshell M, Lane EB and Leigh IM (1989) Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci 94: 403-413
40. van Roozendaal CEP, van Ooijen B, Klijm JGM, Claassen C, Eggermont AMM, Henzen-Logmans SC and Foekens JA (1992) Stromal influences in breast cancer cell growth. Br J Cancer 65: 77-81
41. Vermeulen A, Deslypre JP, Paridaens R, Leclercq G, Roy F and Heuson JC (1986) Aromatase, 17β-hydroxysteroid dehydrogenase and intratissular sex hormone concentrations in cancerous and normal glandular breast tissue in postmenopausal women. Eur J Cancer Clin Oncol 22: 515-525
42. Wolman, SR, Smith HS, Stampfer M and Hackett AJ (1985) Growth of diploid cells from breast cancers. Cancer Genet Cytogenet 16: 49-64
43. Yaswen P, Smoll A, Peehl DM, Trask DK, Sager R and Stampfer MR (1990) Downregulation of a calmodulin-related gene during transformation of human mammary epithelial cells. Proc Natl Acad Sci USA 87: 7360-7364
44. Yaswen P, Smoll A, Hosada J, Parry G and Stampfer MR (1992) Protein product of a human intronless calmodulin-like gene shows tissue-specific expression and reduced abundance in transformed cells. Cell Growth Differen 3: 335-346