Modulation of hepatocyte function in an immortalized human hepatocyte cell line following exposure to liver-failure plasma

Artificial Organs

Volumn 26, Issue 4, 2002, Pages 340-348

  McCloskey, Paschal ^a   Tootle, Rosemary ^a   Selden, Clare ^a   Larsen, Fin ^b   Roberts, Eve ^c   Hodgson, Humphrey J F ^a  

^a ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Bioartificial liver; Cell culture; Hepatocyte; Liver failure

Indexed keywords

ARTIFICIAL ORGANS; BIOSYNTHESIS; CELLS;

BIOARTIFICIAL LIVER; CELL LINES; CULTURE MEDIUM; DNA SYNTHESISS; HEPATOCYTE FUNCTIONS; HEPATOCYTES; HUMAN HEPATOCYTES; LIVER FAILURE; PERFORMANCE; PLASMA MEDIA;

CELL CULTURE;

CYTOCHROME P450; DNA; THYMIDINE;

ARTICLE; CELL ASSAY; CELL FUNCTION; CELL IMMORTALIZATION; CELL LINE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY TEST; DEVICE; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME ASSAY; HUMAN; HUMAN CELL; LIVER CELL; LIVER CELL CULTURE; LIVER FAILURE; OXIDATIVE STRESS; PERFORMANCE; PLASMA; PLASMAPHERESIS; PRIORITY JOURNAL; PROTEIN SYNTHESIS; TOXICITY;

EID: 0036237187     PISSN: 0160564X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1525-1594.2002.06918.x     Document Type: Article

References (32)

1. Transport and metabolism in the hepatobiliary system
2. Microcarrier culture of hepatocytes in whole plasma for use in liver support bioreactors
3. Artifical liver: Evolution and future perspectives
4. Culture of a differentiated liver cell line, Hep G2, in serum with application to a bioartificial liver: Effect of supplementation of serum with amino acids
5. The effects of serum from patients with acute liver failure on the growth and metabolism of Hep G2 cells
6. Hepatotoxic effects of sera from patients with fulminant hepatitis B on isolated rat hepatocytes in culture
7. Plasma cytokine levels and coagulation and complement activation during use of the extracorporeal liver assist device in acute liver failure
8. Cytotoxicity of bile in human Hep G2 cells and in primary cultures of rat hepatocytes
9. Establishment of a human hepatocyte line derived from primary culture in a collagen gel sandwich culture system
10. Resistance of three immortalized human hepatocyte cell lines to acetaminophen and N-acetyl-p-benzoquinoneimine toxicity
11. 51Cr release by isolated rat hepatocytes: An indicator of membrane damage
12. Heat shock proteins protect WEHI-164 target cells from the cytolysis by tumor necrosis factor α and β
13. Increased gluathione in cultured hepatocytes associated with induction of cytochrome P-450
14. Characterization of human hepatocye lines derived from normal liver tissue
15. A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96 well plates
16. Caffeine biotransformation in human hepatocyte lines derived from normal liver tissue
17. Biotransformation of caffeine, paraxanthine, theophylline and theobromine by polycyclic aromatic hydrocarbon-inducilble cytochrome(s) P450 in human liver microsomes
18. Effect of culture medium on the maintenance of steroid metabolism in cultured adult rat hepatocytes
19. Irreversible androgenic programming at birth of microsomal and soluble rat liver enzymes active on 4-androstene-3,17-dione and 5a-androstane-3a,17bdiol
20. Protein measurement with the Folin phenol reagent
21. In vitro plasma perfusion through adsorbents and plasma ultrafiltration to remove endotoxins and cytokines
22. Non-parenchymal cells from regenerating rat liver generate IL-lα and IL-lβ - A mechanism of negative regulation of hepatocyte proliferation
23. Plasma amino acid profiles in patients with fulminant hepatic failure treated by repeated polyacrylonitrile membrane hemodialysis
24. Regulation of γ-glutamylcysteine synthetase subunit gene expression in retinal Muller cells by oxidative stress
25. Cytokines impair microsomal enzyme detoxification in isolated hepatocytes: Implications for a bioartifical liver
26. Plasma hepatocyte growth factor and biliprotein levels and outcome in fulminant hepatic failure
27. Significantly improved survival time in pigs with complete liver ischemia treated with a novel bioartifical liver
28. Clinical experience with artificial liver support systems
29. Extracorporeal liver support: Cell-based therapy for the failing liver
30. Biocompatibility of a cuprophane charcoal-based detoxification device in cirrhotic patients with hepatic encephalopathy
31. Comparison of three solid phase supports for promoting three-dimensional growth and function of human liver cell lines
32. Three-dimensional in vitro cell culture leads to a marked upregulation of cell function in human hepatocyte cell lines - An important tool for the development of a bioartificial liver machine