The effects of serum from patients with acute liver failure on the growth and metabolism of Hep G2 cells

Artificial Organs

Volumn 22, Issue 12, 1998, Pages 1023-1030

  Shi, Q ^a   Gaylor, J D S ^a   Cousins, R ^a   Plevris, J ^b   Hayes, P C ^b   Grant, M H ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Bioartificial liver; Cytotoxicity of fulminant hepatic failure serum; Hep G2 cells

Indexed keywords

ARTIFICIAL ORGANS; BIOSYNTHESIS; BODY FLUIDS; CELL PROLIFERATION; NUCLEIC ACIDS;

ACUTE LIVER FAILURE; BIOARTIFICIAL LIVER; CYTOTOXICITY OF FULMINANT HEPATIC FAILURE SERUM; DIRECT CONTACT; DNA-PROTEINS; FULMINANT HEPATIC FAILURES; GLUTHATHIONE; HEP-G2 CELLS; HOMOEOSTASIS; LIVER CELLS;

CELL CULTURE;

DNA; GLUTATHIONE; PROTEIN; RNA;

ARTICLE; ARTIFICIAL LIVER; CELL PROLIFERATION; CONTROLLED STUDY; CYTOTOXICITY; DETOXIFICATION; DEVICE; HEP 2 CELL; HUMAN; HUMAN CELL; LIVER FAILURE; PRIORITY JOURNAL;

EID: 0031646704     PISSN: 0160564X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1525-1594.1998.06211.x     Document Type: Article

References (32)

1. Kay MA, Fausto N. Liver regeneration: Prospects for therapy based on new technology. Mol Med Today 1997;March: 108-15.
2. Rozga J, Demetriou AA. Artificial liver: Evolution and future perspective. ASAIO J 1995;41:831-7.
3. Nyberg SL, Peshwa M, Payne WD, Hu WS, Cerra FB. Evolution of the bioartificial liver: The need for randomized clinical trials. Am J Surg 1993;166:512-21.
4. Sussman NL, Lake JR. Treatment of hepatic failure 1996: Current concepts and progress toward liver dialysis. Am J Kidney Dis 1996;27:605-21.
5. Hughes RD, Cochrane AMG, Thomson AD, Murray-Lyon IM, Williams R. The cytotoxicity of plasma from patients with acute hepatic failure to isolated rabbit hepatocytes. Br J Exp Pathol 1976;57:348.
6. Gover CD, Hughes RD, Williams R. Rapid inhibition of DNA synthesis in hepatocytes from regenerating livers by serum from patients with fulminant hepatic failure. Br J Exp Palhol 1982;63:547-53.
7. Sewell RB, Hughes RD, Poston L, Williams R. Effects of serum from patients with fulminant hepatic failure on leucocyte sodium transport. Clin Sci 1982;63:237-42.
8. Lee WM. Acute liver failure. New Engl J Med 1993;329: 1862-74.
9. Doostdar H, Duthie SJ, Melvin WT, Burke MD, Gram MH. Influence of medium composition on the drug metabolising enzyme activities of human Hep G2 hepatoma cells. Fed Eur Biochem Soc Lett 1988;241:15-8.
10. Grant MH, Burke MD, Hawksworth GM, Duthie SJ, Engeset J, Petrie JC. Human adult hepatocytes in primary culture: Maintenance of mixed function oxidase and conjugation pathways of drug metabolism. Biochem Pharmacol 1987;36: 2311-6.
11. Sussman NL, Kelly JH. Extracorporeal liver assist in the treatment of fulminant hepatic failure. Blood Purif 1993;11: 170-4.
12. Sussman NL, Gislason GT, Conlin CA, Kelly JH. The hepatix extracorporeal liver assist device: Initial clinical experience. Artif Organs 1994;18:390-6.
13. Habig WH, Jakoby WB. Assays for differentiation of glutathione-S-transferase. Methods Enzymol 1981;77:398-405.
14. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-3.
15. Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 1985;113:484-90.
16. Phillips AH, Langdon RG. Hepatic triphosphopyridine nucleotide-cytochrome c reductase: Isolation, characterisation and kinetic studies. J Biol Chem 1962;237:2652-660.
17. Grant MH, Rodger IW. The metabolism of leukotrienes, detoxification of reactive oxygen species and the activity of the cytochrome P450 mixed function oxidase system in sensitised guinea pig lungs. Pulm Pharmacol 1991;4:165-9.
18. Rister M, Baehner RL. The alteration of Superoxide dismutlase, catalase, glutathione peroxidase and NADPH cytochrome c reductase in guinea pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia. J Clin Invest 1976;58:1174-84.
19. Anuforo DC, Acosta D, Smith RV. Hepatotoxicity studies with primary cultures of liver cells. In Vitro 1978;14:981-8.
20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
21. Hissin PJ, Hilf R. A fluorimetric method for the determination of oxidised and reduced glutathione in tissues. Annal Biochem 1976;74:214-26.
22. Doostdar H, Demoz A, Burke MD, Melvin WT, Grant MH. Variation in drug-metabolizing enzyme activities during the growth of human Hep G2 cells. Xenobiotica, 1990;20:435-41.
23. Gazzard BG, Portmann B, Murray-Lyon IM, Williams R. Causes of death from fulminant hepatic failure and relationship to quantitative histological assessment of parenchymal damage. Q J Med 1975;176:615-26.
24. Burt AD, Fames OFW. Pathophysiology of the liver. In: MacSween RNM, Schenlr APP, Burt AD, Portmann BC, eds. Pathology of the liver. London: Churchill Livingstone Press 1994:65-8.
25. Haas T, Holloway CJ, Osterthum V, Trautschold I. Hepatotoxic effects of sera from patients with fulminant hepatitis B on isolated rat hepatocytes in culture. J Clin Chem Clin Biochem 1981;19:283-6.
26. Hughes RD, Zhang L, Tsubouchi H, Daikuhara Y, Williams R. Plasma hepatocyte growth factor and biliprotein levels and outcome in fulminant hepatic failure. J Hepatol 1994;20: 106-11.
27. Tystruo N, Jensen SA, Krog B, Dalholf K. Expression of liver-specific functions in rat hepatocytes following sublethal and lethal acetaminophen poisoning. J Hepatol 1996;25: 183-90.
28. Meister A. Glutathione. In: Arias IM, Jakoby WB, Popper H, Schachler D, Schatritz DA, eds. Liver: Biology and pathobiology, 2nd Edition. New York: Raven Press 1988:401-7.
29. Melkonian AD, Gaylor JDS, Cousins RB, Grant MH. Culture of a differentiated liver cell line. Hep G2, in serum with application to a bioartificial liver: Effects of supplementation of serum with amino acids. Artif Organs 1994;18:611-7.
30. Grant MH, Smirthwaite AD, Cousins RB, Gaylor JDS. The cytotoxicity of bile on human Hep G2 cells. Cell Biol Toxicol 1996:34.
31. Ekevall E, Plevris J, Smith MD, Anderson K, Nelson L, Hayes PC, Cousins RB, Grant MH, Gaylor JDS. Response of rat hepatocytes in culture to serum from fulminant hepatic failure patients. Artif Organs 1997;21:204.
32. Uchino J, Matsue H, Takahashi M, Nakajima Y, Matsushita M, Hamada T, Hashimura E. Function of cultured monolayer pig hepatoeytes in plasma from hepatic failure patients. ASAIO Trims 1991;37:M337-8.