Distance dependence and salt sensitivity of pairwise, coulombic interactions in a protein

Protein Science

Volumn 11, Issue 5, 2002, Pages 1004-1016

  Lee, Kelly K ^a   Fitch, Carolyn A ^a   Bertrand Garcia Moreno E ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Coulombic interactions; Determinants of pKa values; Electrostatics; Ionic strength; Poisson Boltzmann; Salt effects; Staphylococcal nuclease

Indexed keywords

HISTIDINE; NUCLEASE; PROTEIN; SODIUM CHLORIDE;

ARTICLE; CALCULATION; DIELECTRIC CONSTANT; ELECTRICITY; IONIC STRENGTH; MEASUREMENT; MUTANT; PKA; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTON NUCLEAR MAGNETIC RESONANCE; SENSITIVITY AND SENSIBILITY; STAPHYLOCOCCUS; STRUCTURE ANALYSIS; WATER IMMERSION;

ELECTROSTATICS; HISTIDINE; MAGNETIC RESONANCE SPECTROSCOPY; MICROCOCCAL NUCLEASE; MUTATION; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP;

STAPHYLOCOCCUS;

EID: 0036228118     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.4700102     Document Type: Article

References (53)

1. Protein stability and electrostatic interactions between solvent exposed charged side chains
2. NMR assignments of the 4 histidines of staphylococcal nuclease in native and denatured states
3. Prediction of pH-dependent properties of proteins
4. as in proteins
5. a's of ionizable groups in proteins: Atomic detail from a continuum electrostatic model
6. Electrostatic interactions across a beta-sheet
7. Histidine pKa shifts accompanying the inactivating Asp121Asn substitution in a semisynthetic bovine pancreatic ribonuclease
8. Contributions of engineered surface salt bridges to the stability of T4 lysozyme determined by directed mutagenesis
9. Cumulative site-directed charge-change replacements in bacteriophage T4 lysozyme suggest that long-range electrostatic interactions contribute little to protein stability
10. Electrostatics and diffusion of molecules in solution-simulations with the University of Houston Brownian Dynamics program
11. High apparent dielectric constants in the interior of a protein reflect water penetration
12. Binding of buried structural water increases the flexibility of proteins
13. Coulombic effects of remote subsites on the active site of ribonuclease A
14. a values of buried residues: Analysis with continuum methods and role of water penetration
15. Theoretical and experimental analysis of ionization equilibria in ovomucoid third domain
16. a values in the ovomucoid third domain to charge replacement at a neighboring residue
17. a calculations
18. Multiple-site titration and molecular modeling: Two rapid methods for computing energies and forces for ionizable groups in proteins
19. Use of glass electrodes to measure acidities in deuterium oxide
20. Increasing protein stability by altering long-range coulombic interactions
21. The crystal structure of staphylococcal nuclease refined at 1.7 Å resolution
22. Thermal versus guanidine-induced unfolding of ubiquitin. An analysis in terms of the contributions from charge-charge interactions to protein stability
23. Salt effects on ionization equilibria of histidines in myoglobin
24. Binding of Ca2+ to Calbinding D9K: Structural stability and function at high salt concentration
25. a values and the pH dependent stability of the N-terminal domain of L9 as probes of electrostatic interactions in the denatured state. Differentiation between local and nonlocal interactions
26. Electrostatic effects in highly changed proteins: Salt sensitivity of pKa values of histidines in staphylococcal nuclease
27. Long-range surface charge-charge interactions in proteins. Comparison of experimental results with calculations from a theoretical method
28. Engineering a thermostable protein via optimization of charge-charge interactions on the protein surface
29. Observation of histidine residues in proteins by means of nuclear magnetic resonance spectroscopy
30. pH-dependent properties in proteins
31. Contributions of the ionizable amino acids to the stability of staphylococcal nuclease
32. Thermodynamic study of the acid denaturation of bamase and its dependence on ionic strength: Evidence for residual electrostatic interactions in the acid/thermally denatured state
33. a values of the denatured state are on average 0.4 units lower than those of model compounds
34. Charge-charge interactions influence the denatured state ensemble and contribute to protein stability
35. Two exposed amino acid residues confer thermostability on a cold shock protein
36. Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering
37. To charge or not to charge?
38. as and protein stability
39. What are the dielectric "constants" of proteins and how to validate electrostatic models?
40. Estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles
41. a's of ionizable residues in proteins: Semi-microscopic and microscopic approaches
42. The effect of protein relaxation on charge-charge interactions and dielectric constants of proteins
43. The effect of net charge on the solubility, activity, and stability of ribonuclease Sa
44. Rational modification of protein stability by the mutation of charged surface residues
45. Hydrogen-bonds and the pH-dependence of ovomucoid 3rd domain stability
46. a-values in the denatured states of proteins
47. a changes in a protein tyrosine phosphatase: Experimental and theoretical determination of electrostatic properties in a small protein
48. a calculations: Conformational averaging versus the average structure
49. Calculation of the electric potential in the active site cleft due to a-helix dipoles
50. pH dependence of stability of staphylococcal nuclease: Evidence of substantial electrostatic interactions in the denatured state
51. Conformation and hydrogen ion titration of proteins: A continuum electrostatic model with conformational flexibility