Increasing protein stability by polar surface residues: Domain-wide consequences of interactions within a loop

Biophysical Journal

Volumn 82, Issue 1, 2002, Pages 391-398

  Pokkuluri, P R ^a   Raffen, R ^a   Dieckman, L ^a   Boogaard, C ^a   Stevens, F J ^a   Schiffer, M ^a  

^a ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBONYL DERIVATIVE; IMMUNOGLOBULIN LIGHT CHAIN;

AMINO ACID SEQUENCE; ARTICLE; HYDROGEN BOND; MUTANT; MUTATION; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; SURFACE PROPERTY; THERMODYNAMICS; X RAY DIFFRACTION;

EID: 0036217934     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)75403-9     Document Type: Article

References (44)

1. Immunoglobulin light chain amyloidosis: The archetype of structural and pathogenic variability
2. Identification of residues that stabilize the single-chain Fv of monoclonal antibodies B3
3. Crystallography and NMR system: A new software suite for macromolecular structure determination
4. Conformational stability of mixed disulfide derivatives of β-lactoglobulin B
5. The molecular structure of a dimer composed of the variable portions of the Bence-Jones protein Rei refined at 2.0-Å resolution
6. Energetics of a hydrogen bond (charged and neutral) and of a cation-π interaction in apoflavodoxin
7. Principles of protein stability derived from protein engineering experiments
8. v the variable domain of a human immunoglobulin κ light chain
9. A test of the "jigsaw puzzle" model for protein folding by multiple methionine substitutions within the core of T4 lysozyme
10. A general method of in vitro preparation and specific mutagenesis of DNA fragments: Study of protein and DNA interactions
11. Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting
12. v
13. Rapid and efficient site-specific mutagenesis without phenotypic selection
14. Stabilization of protein structures
15. Context-dependent protein stabilization by methionine-to-leucine substitution shown in T4 lysozyme
16. Structural and genetic analysis of protein stability
17. Hydrogen bonding stabilizes globular proteins
18. AMoRe: An automated package for molecular replacement
19. Processing of x-ray diffraction data collected in oscillation mode
20. Single surface stabilizer
21. Two exposed amino acid residues confer thermostability on a cold shock protein
22. A domain flip as a result of a single amino-acid substitution
23. Amino-acid substitutions in a surface turn modulate protein stability
24. Physicochemical consequences of amino acid variations that contribute to fibril formation by immunoglobulin light chains
25. Small zone, high-speed gel filtration chromatography to detect protein aggregation associated with light chain pathologies
26. CHAIN: A crystallographic modeling program
27. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase
28. Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl α-chymotrypsin using different denaturants
29. Refined crystal structure of a recombinant immunoglobulin domain and a complementarity-determining region 1-grafted mutant
30. Sequence statistics reliably predict stabilizing mutations in a protein domain
31. Analysis of protein-protein interaction by simulation of small-zone size-exclusion chromatography: Application to an antibody-antigen association
32. Analysis of protein-protein interaction by simulation of small-zone size exclusion chromatography
33. Four structural risk factors identify most fibril-forming kappa light chains
34. Computer simulation of protein self-association during small-zone gel filtration: Estimation of equilibrium constant
35. Contribution of surface salt bridges to protein stability
36. Contribution of hydrogen bonds to the conformational stability of human lysozyme: Calorimetry and x-ray analysis of six Ser→Ala mutants
37. X-ray crystallography reveals stringent conservation of protein fold after removal of the only disulfide bridge from a stabilized immunoglobulin variable domain
38. v mutant T39K: Application of local NCS restraints
39. The Puc plasmids, an M13Mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
40. Crystal structure of Bence Jones protein Rhe (3 Å) and its unique domain-domain association
41. Additivity of mutational effects in proteins
42. Recombinant immunoglobulin variable domains generated from synthetic genes provide a system for in vitro characterization of light chain amyloid proteins
43. In vitro evolution of thermodynamically stable turns