Quantification of fibronectin adsorption to silicone-rubber cell culture substrates

BioTechniques

Volumn 32, Issue 4, 2002, Pages 876-887

  Cunningham, James J ^a   Nikolovski, Janeta ^a   Linderman, Jennifer J ^a   Mooney, David J ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION; ADSORPTION; BIOLOGICAL MEMBRANES; CELL CULTURE; POLYSTYRENES; PROTEINS; SILICONES;

MECHANICAL FORCE;

RUBBER;

FIBRONECTIN; MATRIX PROTEIN; POLYSTYRENE; SILASTIC;

ADSORPTION; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL ADHESION; CELL CULTURE; CONTROLLED STUDY; DENSITY; DEVICE; EXTRACELLULAR MATRIX; FORCE; MECHANICAL STRESS; NONHUMAN; QUANTITATIVE ANALYSIS; RAT; SMOOTH MUSCLE FIBER;

ANIMALIA;

EID: 0036216212     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02324rr05     Document Type: Article

References (30)

1. Strain measurements in cultured vascular smooth muscle cells subjected to mechanical deformation
2. Focal adhesions: Transmembrane junctions between the extracellular matrix and the cytoskeleton
3. Effects of mechanical forces on signal transduction and gene expression in endothelial cells
4. Adsorption and elution of extracellular matrix proteins on nontissue culture petri dishes
5. Strain profiles for circular cell culture plates containing flexible surfaces employed to mechanically deform cells in vitro
6. Cellular adhesiveness and extracellular substrata
7. Adsorption characteristics of plasma fibronectin in relationship to biological activity
8. Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum-containing medium
9. A method for inducing equi-biaxial and uniform strains in elastomeric membranes used as cell substrates
10. Fibronectin controls capillary endothelial cell growth by modulating cell shape
11. Tensegrity: The architectural basis of cellular mechanotransduction
12. Shear stress activates p60src-Ras-MAPK signaling pathways in vascular endothelial cells
13. Cyclic mechanical strain regulates the development of engineered smooth muscle tissue
14. An equibiaxial strain system for cultured cells
15. Extracellular signal-regulated kinase and c-Jun NH2-terminal kinase activation by mechanical stretch is integrin pendent and matrix-specific in rat cardiac fibroblasts
16. An RGD spacing of 440 nm is sufficient for integrin α V β 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation
17. Attachment of cultured human bone cells to novel polymers
18. Visualization of cellular focal contacts using a monoclonal antibody to 80 kD serum protein adsorbed on the substratum
19. Smooth muscle cell adhesion to tissue engineering scaffolds
20. Microtubule assembly is regulated by externally applied strain in cultured smooth muscle cells
21. Development and characterization of a cloned rat pulmonary arterial smooth muscle cell line that maintains differentiated properties through multiple subcultures
22. Device for the application of a dynamic biaxially uniform and isotropic strain to a flexible cell culture membrane
23. Regulation of cellular response to mechanical signals by matrix design
24. Strain increases airway smooth muscle cell proliferation
25. A strain device imposing dynamic and uniform equi-biaxial strain to cultured cells
26. Mechanical stress stimulates aortic endothelial cells to proliferate
27. A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin
28. Effects of polystyrene surface chemistry on the biological activity of solid phase fibronectin and vitronectin, analyzed with monoclonal antibodies
29. Mechanical forces and their second messengers in stimulating cell growth in vitro
30. Mechanical strain of rat vascular smooth muscle cells is sensed by specific extracellular matrix/integrin interactions