Production of a freeze-thaw-stable potato starch by antisense inhibition of three starch synthase genes

Nature Biotechnology

Volumn 20, Issue 3, 2002, Pages 295-299

  Jobling, Stephen A ^a   Westcott, Roger J ^a   Tayal, Akash ^b   Jeffcoat, Roger ^b   Schwall, Gerhard P ^c  

^a UNILEVER RESEARCH   (United Kingdom)

^b NATIONAL STARCH AND CHEMICAL COMPANY   (United States)

^c PROTEOSYS AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FREEZING; GENES; STARCH; TEXTURES; THAWING;

ANTISENSE INHIBITION;

BIOTECHNOLOGY;

AMYLASE; AMYLOPECTIN; COMPLEMENTARY DNA; STARCH; STARCH SYNTHASE;

ARTICLE; CARBOHYDRATE ANALYSIS; CHEMICAL MODIFICATION; DNA MODIFICATION; ENZYME ACTIVITY; ENZYME ASSAY; FREEZING; GENE EXPRESSION; GENE EXPRESSION REGULATION; POTATO; PRIORITY JOURNAL; THAWING; VISCOMETRY; VISCOSITY;

AMYLOPECTIN; AMYLOSE; CHROMATOGRAPHY; FREEZING; OLIGONUCLEOTIDES, ANTISENSE; PLANTS, GENETICALLY MODIFIED; SOLANUM TUBEROSUM; STARCH; STARCH SYNTHASE; TIME FACTORS;

SOLANUM TUBEROSUM;

EID: 0036195311     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0302-295     Document Type: Article

References (18)

1. Understanding and influencing starch biochemistry
2. Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs
3. A minor form of starch branching enzyme in potato (Solanum tuberosum L.) tubers has a major effect on starch structure: Cloning and characterisation of multiple forms of SBE A
4. Production of very-high amylose potato starch by inhibition of SBE A and B
5. Biochemical and molecular characterisation of a novel starch synthase from potato tubers
6. Cloning and functional analysis of a cDNA encoding a novel starch synthase from potato (Solanum tuberosum)
7. Identification of the major starch synthase in the soluble fraction of potato tubers
8. A combined reduction in activity of starch synthases II and III of potato has novel effects on the starch of tubers
9. Simultaneous antisense inhibition of two starch synthase isoforms in potato tubers leads to accumulation of grossly modified amylopectin
10. Evidence that amylose synthesis occurs within the matrix of the starch granule in potato tubers
11. Field evaluation of transgenic potato plants expressing an antisense granule-bound starch synthase gene: Increase of the antisense effect during tuber growth
12. Determination of water separation from cooked starch and flour pastes after refrigeration and freeze-thaw
13. Cloning and functional analysis of a cDNA encoding a starch synthase isoform from potato (Solanum tuberosum L.) that is predominantly expressed in leaf tissue
14. Crystallisation of malto-oligosaccharides as models of the crystalline form of starch: Minimum chain length required for the formation of double helices
15. Influence of chain-length of short monodisperse amyloses on the formation of A-type and B-type x-ray-diffraction patterns
16. Consequences of antisense RNA inhibition of starch branching enzyme activity on properties of potato starch]