Cloning and functional analysis of a cDNA encoding a starch synthase from potato (Solanum tuberosum L.) that is predominantly expressed in leaf tissue

Planta

Volumn 208, Issue 4, 1999, Pages 503-511

  Kossmann, Jens ^a   Abel, Gernot J W ^a,b   Springer, Franziska ^a   Lloyd, James R ^a   Willmitzer, Lothar ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

^b DANISCO A S   (Denmark)

Author keywords

Antisense potato; Isoform (starch synthase); Solanum (starch synthase); Starch structure; Transgenic potato; Tuber

Indexed keywords

AMINO ACID SEQUENCE; ANTISENSE TECHNOLOGY; COMPLEMENTARY DNA; ENZYME ACTIVITY; GEL ELECTROPHORESIS; GENE EXPRESSION; MOLECULAR CLONING; STARCH SYNTHASE; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; MOLECULAR SEQUENCE DATA; PLANT LEAVES; SEQUENCE HOMOLOGY, AMINO ACID; SOLANUM TUBEROSUM; STARCH SYNTHASE;

EID: 0033150133     PISSN: 00320935     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004250050587     Document Type: Article

References (38)

1. Abel GJW, Springer F, Willmitzer L, Kossmann J (1996) Cloning and functional analysis of a cDNA encoding a novel 139 kDa starch synthase from potato (Solanum tuberosum L.) Plant J 10: 981-991
2. Amasino RM (1986) Acceleration of nucleic acid hybridization rate by polyethylene glycol. Anal Biochem 152: 304-307
3. Baba T, Nishihara M, Mizuno K, Kawasaki T, Shimada H, Kobayashi E, Ohnishi S, Tanaka K-I, Arai Y (1993) Identification, cDNA cloning, and gene expression of soluble starch synthase in rice (Oryza saliva L.) immature seeds. Plant Physiol 103: 565-573
4. Becker D (1990) Binary vectors which allow the exchange of plant selectable markers and reporter genes. Nucleic Acids Res 18: 203
5. Craig J, Lloyd JR, Tomlinson K, Barber L, Edwards A, Wang TL, Martin C, Hedley CL, Smith AM (1998) Mutations in the gene encoding starch synthase II profoundly alter amylopectin structure in pea embryos. Plant Cell 10: 413-426
6. Creuzat-Sigal N, Latil-Damotte M, Cattaneo J, Puig J (1972) Genetic analysis and biochemical characterization of mutants impairing glycogen metabolism in Escherichia coli K-12. In: Piras R, Pontis HG (eds) Biochemistry of the glycoside linkage. Academic Press, New York, pp 647-680
7. Denyer K, Barber LM, Edwards EA, Smith AM, Wang TL (1997) Two isoforms of the GBSSI class of granule bound starch synthase are differentially expressed in the pea plant (Pisum sativum L.) Plant Cell Environ 20: 1566-1572
8. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387-395
9. Dry I, Smith A, Edwards A, Bhattacharyya M, Dunn P, Martin C (1992) Characterization of cDNAs encoding two isoforms of granule-bound starch synthase which show differential expression in developing storage organs of pea and potato. Plant J 2: 193-202
10. Edwards A, Marshall J, Sidebottom C, Visser RGF, Smith A, Martin C (1995) Biochemical and molecular characterization of a novel starch synthase from potato tubers. Plant J 8: 283-294
11. Gao M, Wanat J, Stinard PS, James MG, Myers AM (1998) Characterization of dull1, a maize gene coding for a novel starch synthase. Plant Cell 10: 399-412
12. Hawker JS, Ozbun JL, Preiss J (1972) Unprimed starch synthesis by soluble ADPglucose-starch glucosyltransferase from potato tubers. Phytochemistry 11: 1287-1293
13. Hizukuri S, Tabata S, Nikuni Z (1970) Studies on starch phosphate. Part 1. Estimation of glucose-6-phosphate residues in starch and the presence of other phosphate(s). Starch/Stärke 22: 338-343
14. Höfgen R, Willmitzer L (1990) Biochemical and genetic analysis of different palatin isoforms expressed in various organs of potato (Solanum tuberosum L.). Plant Sci 66: 221-230
15. Hovenkamp-Hermelink JHM, de Vries JN, Adamse P, Jacobsen E, Witholt B, Feenstra WJ (1988) Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato. Potato Res 31: 241-246
16. Knight ME, Harn C, Lilley CER, Guan H, Singletary GW, Mu-Forster C, Wasserman BP, Keeling PL (1998) Molecular cloning of starch synthase I from maize (W64) and expression in Escherichia coli. Plant J 14: 613-622
17. Kossmann J, Visser RGF, Müller-Röber B, Willmitzer L, Sonnewald U (1991) Cloning and expression analysis of a potato cDNA that encodes branching enzyme: evidence for co-expression of starch biosynthetic genes. Mol Gen Genet 230: 39-44
18. Kossmann J, Müller-Röber B, Dyer TA, Raines CA, Sonnewald U, Willmitzer L (1992) Cloning and expression analysis of the plastidic fructose-1,6-bisphosphatase coding sequence from potato: circumstantial evidence for the import of hexoses into chloroplasts. Planta 188: 7-12
19. Lehrach H, Diamond D, Wozney JM, Boedtker H (1977) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16: 4743-4751
20. Lloyd JR, Landschütze V, Kossmann J (1999) Simultanous antisense inhibition of two starch synthase isoforms in potato tubers leads to accumulation of grossly modified amylopectin. Biochem J, 338: 515-521
21. Logemann J, Schell J, Willmitzer L (1987) Improved method for the isolation of RNA from plant tissue. Anal Biochem 163: 16-20
22. Lorberth R, Ritte G, Willmitzer L, Kossmann J (1998) Inhibition of a starch-granule-bound protein leads to modified starch and repression of cold sweetening. Nature Biotech 16: 473-477
23. Marshall J, Sidebottom C, Debet M, Martin C, Smith AM, Edwards A (1996) Identification of the major starch synthase in the soluble fraction of potato tubers. Plant Cell 8: 1121-1135
24. Martin C, Smith AM ( 1995) Starch biosynthesis. Plant Cell 7:971-985
25. 31P NMR. Starch/ Stärke 43: 25
26. Müller-Röber B, Kossmann J, Hannah LC, Willmitzer L, Sonnewald U (1990) Only one of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose. Mol Gen Genet 224: 136-146
27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497
28. Nielsen TH, Wischmann B, Enevoldsen K, Møller BL (1994) Starch phosphorylation in potato tubers proceeds concurrently with de novo biosynthesis of starch. Plant Physiol 105: 111-117
29. Preiss J (1991) Biology and molecular biology of starch synthesis and its regulation. In: Miflin BJ, Miflin HF (eds) Oxford surveys of plant molecular and cell biology, vol 7. Oxford University Press, Oxford, pp 59-114
30. Rocha-Sosa M, Sonnewald U, Frommer W, Stratmann M, Schell J, Willmitzer L (1989) Both developmental and metabolic signals activate the promoter of class I patatin gene. EMBO J 8: 23-29
31. Schwartz M (1966) Location of the maltose A and maltose B loci on the genetic map of Escherichia coli. J Bacteriol 92: 1083-1089
32. Shewmaker CK, Boyer CD, Wiesenborn DP, Thompson DB, Boersig MR, Oakes JV, Stalker DM (1994) Expression of Escherichia coli glycogen synthase in the tubers of transgenic potatoes (Solanum tuberosum) results in a highly branched starch. Plant Physiol 104: 1159-1166
33. Stitt M, Mac Lilley R, Gerhardt R, Heldt HW (1989) Metabolite levels in specific cells and subcellular compartments of plant leaves. Methods Enzymol 174: 518-552
34. Tacke M, Yi L, Steup M (1991) Multiplicity of soluble glucan-synthase activity in spinach leaves: Enzyme pattern and intracellular location. Planta 185: 220-226
35. Tomlinson KL, Lloyd JR, Smith AM (1997) Importance of isoforms of starch-branching enzyme in determining the structure of starch in pea leaves. Plant J 11: 31-43
36. Van der Leij FR, Visser RGF, Ponstein AS, Jacobsen E, Feenstra WJ (1991) Sequence of the structural gene for the granule-bound starch synthase of potato (Solanum tuberosum L.) and evidence for a single point deletion in the amf allele. Mol Gen Genet 228: 240-248
37. Von Schaewen A (1989) Untersuchungen zur ER-vermittelten, subzellulären Kompartimentierung fremder Proteine in höheren Pflanzen. PhD thesis, Freie Universität Berlin
38. Zeeman SC, Umemoto T, Lue W-L, Au-Yeung P, Martin C, Smith AM, Chen J (1998) A mutant of Arabidopsis lacking a chloroplastic isoamylase accumulates both starch and phytoglycogen. Plant Cell 10: 1699-1711