Cloning and functional analysis of a cDNA encoding a novel 139 kDa starch synthase from potato (Solanum tuberosum L.)

Plant Journal

Volumn 10, Issue 6, 1996, Pages 981-991

  Abel, Gernot J W ^a   Springer, Franziska ^a,b   Willmitzer, Lothar ^a,b   Kossmann, Jens ^a,b  

^a Inst F Genbiologische Forschung   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

SOLANUM TUBEROSUM; TUBEROSUM;

EID: 0030470475     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.1996.10060981.x     Document Type: Article

References (52)

1. Abel, G.J.W. (1995) Untersuchungen zur Funktion von Stärke-Synthasen in der Kartoffel (Solanum tuberosum L.). PhD Thesis. Berlin: Freie Universität.
2. Amasino, R.M. (1986) Acceleration of nucleic acid hybridization rate by polyethylene glycol. Anal. Biochem. 152, 304-307.
3. Baba, T., Noro, M., Hiroto, M. and Arai, Y. (1990) Properties of primer-dependent starch synthesis catalyzed by starch synthase from potato tubers. Phytochemistry 29, 719-723.
4. Baba, T., Nishihara, M., Mizuno, K., Kawasaki, T., Shimada, H., Kobayashi, E., Ohnishi, S., Tanaka, K.-I. and Arai, Y. (1993) Identification, cDNA cloning, and gene expression of soluble starch synthase in rice (Oryza sativa L.) immature seeds. Plant. Physiol. 103, 565-573.
5. Becker, D. (1990) Binary vectors which allow the exchange of plant selectable markers and reporter genes. Nucl. Acids Res. 18, 203.
6. Boyer, C.D. and Preiss, J. (1981) Evidence for independent genetic control of the multiple forms of maize endosperm branching enzymes and starch synthases. Plant Physiol. 67, 1141-1145.
7. Bradford, M.M. (1976) Rapid and quantitative method for quantitation of microgram quantities of protein utilizing the principle protein-dye binding. Anal. Biochem. 72, 248-252.
8. Delrue, B., Fontaine, T., Routier, F., Decq, A., Wieruszeski, J.M., van der Koornhuyse, N., Maddelein, M.L., Fournet, B. and Ball, S. (1992) Waxy Chlamydomonas reinhardtii monocellular algal mutants defective in amylose biosynthesis and granule-bound starch synthase activity accumulate structurally modified amylopectin. J. Bacteriol. 174, 3612-3620.
9. Denyer, K., Hylton, C.M., Jenner, C.F. and Smith, A.M. (1995) Identification of multiple isoforms of soluble and granule-bound starch synthase in developing wheat endosperm. Planta, 196, 256-265.
10. Devereux, J., Haeberli, P. and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucl. Acids Res. 12, 387-395.
11. Dry, I., Smith, A., Edwards, A., Bhattacharyya, M., Dunn, P. and Martin, C. (1992) Characterization of cDNAs encoding two isoforms of granule-bound starch synthase which show differential expression in developing storage organs of pea and potato. Plant J. 2, 193-202.
12. Echt, C.S. and Schwartz, D. (1981) Evidence for the inclusion of controlling elements within the structural gene at the waxy locus in maize. Genetics, 99, 275-284.
13. Edwards, A., Marshall, J., Sidebottom, C., Visser, R.G.F., Smith, A. and Martin, C. (1995) Biochemical and molecular characterization of a novel starch synthase from potato tubers. Plant J. 8, 283-294.
14. Fontaine, T., D'Hulst, C., Maddelein, M.-L., et al. (1993) Towards an understanding of the biogenesis of the starch granule. J. Biol. Chem. 268, 16223-16230.
15. Hawker, J.S., Ozbun, J.L. and Preiss, J. (1972) Unprimed starch synthesis by soluble ADP glucose-starch glucosyltransferase from potato tubers. Phytochemistryt 11, 1287-1293.
16. Hawker, J.S., Ozbun, J.L., Ozaki, H., Greenberg, E. and Preiss, J. (1974) Interaction of spinach leaf adenosine diphosphate glucose a-1,4-glucan α-4-glycosyl transferase and α-1,4-glucan, α-1,4-glucan-6-glucosyl transferase in synthesis of branched α-glucan. Arch. Biochem. Biophys. 160, 530-551.
17. Hawker, J.S., Marschner, H. and Krauss, A. (1979) Starch synthesis in developing potato tubers. Physiol. Plant. 46, 25-30.
18. Hesse, H. (1993) Molekulare Ansätze zur Analyse von sink und source Situationen in Solarium tuberosum L. und Beta vulgaris L. PhD Thesis. Berlin: Freie Universität.
19. Hizukuri, S., Tabata, S. and Nikuni, Z. (1970) Studies on starch phosphate. Part 1. Estimation of glucose-6-phosphate residues in starch and the presence of other phosphate(s). Starch/Stärke, 22, 338-343.
20. Hovenkamp-Hermelink, J.H.M., Jacobsen, E., Ponstein, A.S., Visser, R.G.F., Vos-Scheperkeuter, G.H., Bijmolt, E.W., de Vries, J.N., Witholt, B. and Feenstra, W.J. (1987) Isolation of an amylose-free starch mutant of the potato (Solanum tuberosum L.) Theor. Appl. Genet. 75, 217-221.
21. Hovenkamp-Hermelink, J.H.M., de Vries, J.N., Adamse, P., Jacobsen, E., Witholt, B. and Feenstra, W.J. (1988) Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue in the potato. Potato Res. 31, 241-246.
22. Hseih, J.-S. (1988) Genetic studies on the wx gene of sorghum (Sorghum bicolor (L.) Moench). I. Examination of the protein product of the waxylocus. Bot. Bull. Acad. Sinica, 29, 293-299.
23. Kossmann, J., Visser, R.G.F., Müller-Röber, B., Willmitzer, L. and Sonnewald, U. (1991) Cloning and expression analysis of a potato cDNA that encodes branching enzyme: evidence for co-expression of starch biosynthetic genes. Mol. Gen. Genet. 230, 39-44.
24. Kossmann, J., Müller-Röber, B., Dyer, T. A., Raines, C.A., Sonnewald, U. and Willmitzer, L. (1992) Cloning and expression analysis of the plastidic fructose-1,6-bisphosphatase codi ng sequence from potato: circumstantial evidence for the import of hexoses into chloroplasts. Planta, 188, 7-12.
25. Kuipers, G. J., Vreem, J.T.M., Meyer, H., Jacobsen, E., Feenstra, W.J. and Visser, R.G.F. (1992) Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato. Euphytica, 59, 83-93
26. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
27. Lehrach, H., Diamond, D., Wozney, J.M. and Boedtker, H. (1977) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry, 16, 4743-4751.
28. van der Leij, F.R., Visser, R.G.F., Ponstein, A.S., Jacobsen, E. and Feenstra, W.J. (1991) Sequence of the structural gene for the granule-bound starch synthase of potato (Solanum tuberosum L.) and evidence for a single point deletion in the amf allele. Mol. Gen. Genet. 228, 240-248.
29. Logemann, J., Schell, J. and Willmitzer, L. (1987) Improved method for the isolation of RNA from plant tissue. Anal. Biochem. 163, 16-20.
30. Matters, G.L. and Boyer, C.D. (1982) Soluble starch syntheses and starch branching enzymes from cotyledons of smooth- and wrinkled-seeded lines of Pisum sativum L. Biochem. Genet. 20, 833-848.
31. Mu, C., Harn, C., Ko, Y.-T., Singletary, G.W., Keeling, P.L. and Wassermann, B.P. (1994) Association of a 76 kDa polypeptide with soluble starch synthase I activity in maize (cv B73) endosperm. Plant J. 6, 151-159.
32. Müller-Röber, B., Kossmann, J., Hannah, L.C., Willmitzer, L. and Sonnewald, U. (1990) Only one of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose. Mol. Gen. Genet. 224, 136-146.
33. 31P NMR. Starch/Stärke 43, 25-27.
34. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497.
35. Nielsen, T. H., Wischmann, B., Enevoldsen, K. and Møller, B. L. (1994) Starch phosphorylation in potato tubers proceeds concurrently with de novo biosynthesis of starch. Plant Physiol. 105, 111-117.
36. Okuno, K. and Sakaguchi, S. (1982) Inheritance of starch characteristics in perisperm of Amaranthus hypocondriacus. J. Hered. 73, 467.
37. Pollock, C.J. and Preiss, J. (1980) The citrate-stimulated starch synthase of starchy maize kernels: purification and properties. Arch. Biochem. Biophys. 204, 578-588.
38. Ponstein, A. (1990) Starch synthesis in potato tubers. PhD Thesis. Groningen, The Netherlands: Rijksuniversiteit.
39. Preiss, J. (1991) Biology and molecular biology of starch synthesis and its regulation. In Oxford Surveys of Plant Molecular and Cell Biology, Volume 7 (Miflin, B.J., ed.). Oxford: Oxford University Press, pp. 59-114.
40. Rocha-Sosa, M., Sonnewald, U., Frommer, W., Stratmann, M., Schell, J. and Willmitzer, L. (1989) Both developmental and metabolic signals activate the promoter of class I palatin gene. EMBO J. 8, 23-29.
41. Rohde, W., Becker D. and Salamini, F. (1988) Structural analysis of the waxy locus from Hordeum vulgare. Nucl. Acids Res. 16, 7185-7186.
42. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
43. von Schaewen, A. (1989) Untersuchungen zur ER-vermittelten, subzellulären Kompartimentierung fremder Proteine in höheren Pflanzen. PhD Thesis. Berlin: Freie Universität.
44. Shewmaker, C.K., Boyer, C.D., Wiesenborn, O.P., Thompson, D.B., Boersig, M.R., Oakes, J.V. and Stalker, D.M. (1994) Expression of Escherichia coli glycogen synthase in the tubers of transgenic potatoes (Solanum tuberosum) results in a highly branches starch. Plant Physiol. 104, 1159-1166.
45. Shulmann, A.H. and Ahokas, H. (1990) A novel shrunken endosperm mutant of barley. Physiol. Plant. 78, 583-589.
46. Stitt, M., MacLilley, R., Gerhardt, R. and Heldt, H.W. (1989) Metabolite levels in specific cells and subcellular compartments of plant leaves. Methods Enzymol. 174, 518-552.
47. Swinkels, J.J.M. (1985) Composition and properties of commercial native starches. Starch/Stärke, 37, 1-5.
48. Tsay, J.S. and Kuo, G.G. (1980) Enzymatic activities of starch synthesis in potato tubers of different sizes. Physiol. Plant. 48, 460-462.
49. Tyynelä, J. and Shulmann, A.H. (1993) An analysis of soluble starch synthase isozy mes from the developing grains of normal and shx cv. Bomi barley (Hordeum vulgare L.). Physiol. Plant 89, 835-841.
50. Tyynelä, J., Stitt, M., Lönneborg, A., Smeekens, S. and Schulmann, A.M. (1995) Metabolism of starch synthesis in developing grains of the shx shrunken mutant of barley (Hordeum vulgare). Physiol. Plant. 93, 77-84.
51. Villareal, C.P. and Juliano, B.O. (1986) Waxy gene factor and residual protein of rice starch granules. Starch/Stärke, 38, 118-119.
52. Visser, R.G.F., Somhorst, I., Kuipers, G. J., Buys, N.J., Feenstra, W.J. and Jacobsen, E. (1991) Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol. Gen. Genet. 225, 289-296.