Multidimensional NMR methods for protein structure determination

IUBMB Life

Volumn 52, Issue 6, 2001, Pages 291-302

  Kanelis, Voula ^a   Forman Kay, Julie D ^a   Kay, Lewis E ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Deuteration; Dipolar couplings; Multidimensional heteronuclear NMR; TROSY

Indexed keywords

ANALYTIC METHOD; COMPLEX FORMATION; MOLECULAR WEIGHT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN INTERACTION; PROTEIN STRUCTURE; REVIEW; STRUCTURE ANALYSIS; TECHNIQUE;

EID: 0036189601     PISSN: 15216543     EISSN: None     Source Type: Journal    
DOI: 10.1080/152165401317291147     Document Type: Review

References (61)

1. The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins
2. NMR spectroscopy of large molecules and multimolecular assemblies in solution
3. New techniques in structural NMR - Anisotropic interactions
4. Solution structure of a Nedd4 WW domain-ENaC peptide complex
5. Structures of larger proteins in solution: Three- and four-dimensional heteronuclear NMR spectroscopy
6. Multidimensional nuclear magnetic resonance methods for protein studies
7. Heteronuclear multidimensional NMR experiments for the sturcture determinatin of proteins in solution employing pulsed field gradients
8. HNCACB, a high sensitivity 3D NMR experiment to correlate amide proton and nitrogen resonances with the α- and β-carbon resonances in proteins
9. Correlating backbone amide and side chain resonances in larger proteins by multiple relayed triple resonance NMR
10. Amino acid type determination in the sequential assignment procedure of uniformly 13C/15N-enriched proteins
11. WW domains of Nedd4 bind to the proline-rich PY motifs in the epithelial Na+ channel deleted in Liddle's syndrome
12. A de novo missense mutation of the beta subunit of the epithelial sodium channel causes hypertension and Liddle syndrome, identifying a proline-rich segment critical for regulation of channel activity
13. A family with Liddle's syndrome caused by a new missense mutation in the beta subunit of the epithelial sodium channel
14. Liddle disease caused by a missense mutation of beta subunit of the epithelial sodium channel gene
15. Genetic analysis of the epithelial sodium channel in Liddle's syndrome
16. Side chain and backbone assignments in isotopically labeled proteins from two heteronuclear triple resonance experiments
17. An efficient triple resonance experiment using carbon 13 isotropic mixing for determining sequence-specific resonance assignments of isotopically -enriched proteins
18. 13C labeled proteins via scalar couplings
19. NMR pulse schemes for the sequence-specific assignment of arginine guanidino 15N and 1H chemical shifts in proteins
20. Validation of protein structure from anisotropic carbonyl chemical shifts in a dilute liquid crystalline phase
21. Measurement of proton, nitrogen and carbonyl chemical shielding anisotropies in a protein dissolved in a dilute phase crystalline phase
22. Identification of the hydrogen bonding network in a protein by scalar couplings
23. NC' scalar couplings
24. Measurement of homo- and heteronuclear J couplings from quantitative J correlation
25. 2O
26. Isotope-filtered 2D NMR of a protein-peptide complex: Study of a skeletal muscle myosin light chain kinase fragment bound to calmodulin
27. Methods for measurement of intermolecular NOEs by multinuclear NMR spectroscopy: Application to a bacteriophage N-peptide/boxB RNA complex
28. 15N-enriched proteins. Application to HIV-1 Nef
29. Use of 1HN - 1HN NOEs to determine protein global folds in perdeuterated proteins
30. Global folds of highly deuterated, methyl protonated proteins by multidimensional NMR
31. Surface, subunit interfaces and interior of oligomeric proteins
32. A robust and cost-effective method for the production of Val, Leu, Ile (delta 1) methyl-protonated 15N-, 13C-, 2H-labeled proteins
33. Solution NMR studies of a 42 kDa Escherichia coil maltose binding protein/ β-cyclodextran complex: Chemical shift assignments and analysis
34. 13C-labeled proteins
35. 13C-labeled proteins with high resolution
36. 2H-labeled proteins
37. 13C-edited NOESY experiment
38. Global folds of proteins with low densities of NOEs using residual dipolar couplings: Application to the 370-residue maltodextrin-binding protein
39. Nuclear magnetic dipole interactions in field-oriented proteins: Information for structure determination in solution
40. Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium
41. Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions
42. Direct structure refinement against residual dipolar couplings in the presence of rhombicity of unknown magnitude
43. Impact of residual dipolar couplings on the accuracy of NMR structures determined from a minimal number of NOE restraints
44. Crystallography and NMR system: A new software suite for macromolecular structure determination
45. Direct structure refinement of high molecular weight proteins against residual dipolar couplings and carbonyl chemical shift changes upon alignment: An application to maltose binding protein
46. Refined 1.8-Å structure reveals the mode of binding of beta-cyclodextrin to the maltodextrin binding protein
47. Orienting domains in proteins using dipolar couplings measured by liquid-state NM R: Differences in solution and crystal forms of maltodextrin binding protein loaded with β-cyclodextrin
48. Ligand-induced structural changes to maltodextrin-binding protein as studied by solution NMR spectroscopy
49. The 2.3-Å resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis
50. What is the average conformation of bacteriophage T4 lysozyme in solution? A domain orientation study using dipolar couplings measured by solution NMR
51. Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution
52. TROSY triple-resonance four-dimensional NMR spectroscopy of a 46 ns tumbling protein
53. 2H labeled proteins
54. Assignment of 1H(N), 15N, 13C(alpha), 13CO and 13C(beta)resonances in a 67 kDa p53 dimer using 4D-TROSY NMR spectroscopy
55. New insights into p53 function from structural studies
56. Crystal structure of a p53 tumor suppressor-DNA complex: Understanding tumorigenic mutations
57. Refined solution structure of the oligomerization domain of the tumour suppressor p53
58. Solution structure of the tetrameric minimum transforming domain of p53
59. Latent and active p53 are identical in conformation