Developmentally-regulated changes of Xist RNA levels in single preimplantation mouse embryos, as revealed by quantitative real-time PCR

Molecular Reproduction and Development

Volumn 61, Issue 4, 2002, Pages 425-436

  Hartshorn, Cristina ^a   Rice, John E ^a   Wangh, Lawrence J ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

Embryo sexing; Genome copy counting; MRNA copy counting; Xist expression

Indexed keywords

MESSENGER RNA; PROTEIN XIST; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL DIFFERENTIATION; CELL DIVISION; CONTROLLED STUDY; ECTODERM; EMBRYO; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENOME IMPRINTING; INNER CELL MASS; MALE; MEASUREMENT; MORULA; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PREIMPLANTATION EMBRYO; PRIORITY JOURNAL; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SRY GENE; X CHROMOSOME; X CHROMOSOME INACTIVATION;

ANIMALS; BLASTOCYST; DNA-BINDING PROTEINS; DOSAGE COMPENSATION, GENETIC; FEMALE; GENE EXPRESSION REGULATION, DEVELOPMENTAL; MALE; MICE; NUCLEAR PROTEINS; POLYMERASE CHAIN REACTION; PREGNANCY; RNA; RNA, UNTRANSLATED; SEX DETERMINATION (ANALYSIS); SEX-DETERMINING REGION Y PROTEIN; TRANSCRIPTION FACTORS;

EID: 0036184156     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/mrd.10037     Document Type: Article

References (63)

1. X inactivation-specific transcript expression in mouse oocytes and zygotes
2. The biochemistry of differentiation of mouse trophoblast: Studies on polyploidy
3. DNA synthesis in the preimplantation mouse embryo
4. Loss of methylation activates Xist in somatic but not in embryonic cells
5. Integrated amplification and detection of the C677T point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves
6. Characterization of a murine gene expressed from the inactive X chromosome
7. The role of Xist in X-inactivation
8. Conservation of position and exclusive expression of mouse Xist from the inactive X chromosome
9. The product of the mouse Xist gene is a 15 kb inactive X-specific transcript containing no conserved ORF and located in the nucleus
10. The human X-inactivation centre is not required for maintenance of X-chromosome inactivation
11. A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome
12. The human XIST gene: Analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus
13. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
14. Quantitative RT-PCR assays show Xist RNA levels are low in mouse female adult tissue, embryos and embryoid bodies
15. Histone macroH2A1 is concentrated in the inactive X chromosome of female mammals
16. Conditional deletion of Xist disrupts histone macroH2A localization but not maintenance of X inactivation
17. XIST expression in human oocytes and preimplantation embryos
18. X inactive-specific transcript (Xist) expression and X chromosome inactivation in the preattachment bovine embryo
19. Quantitative RT-PCR: Pitfalls and potential
20. Lack of coupling between onset of giant transformation and genome endoreduplication in the mural trophectoderm of the mouse blastocyst
21. Xist RNA associates with specific regions of the inactive X chromatin
22. Regulation of X-chromosome inactivation in development in mice and humans
23. Maternally inherited X chromosome is not inactivated in mouse blastocysts due to parental imprinting
24. Real time quantitative PCR
25. A new structure for the murine Xist gene and its relationship to chromosome choice/counting during X-chromosome inactivation
26. The foundation of two distinct cell lineages within the mouse morula
27. Cell subpopulations in the late morula and early blastocyst of the mouse
28. A role for cytoplasmic determinants in the development of the mouse early embryo?
29. Expression of Xist during mouse development suggests a role in the initiation of X chromosome inactivation
30. Imprinting and X chromosome counting mechanisms determine Xist expression in early mouse development
31. Development and DNA polymerase activities in cultured preimplantation mouse embryos: Comparison with embryos developed in vivo
32. Transient Xist-ence
33. Expression of X-linked genes in androgenetic, gynogenetic, and normal mouse preimplantation embryos
34. Effects of X chromosome number and parental origin on X-linked gene expression in preimplantation mouse embryos
35. Disruption of imprinted X inactivation by parent-oforigin effects at Tsix
36. Tsix, a gene antisense to Xist at the X-inactivation centre
37. Gene action in the X chromosome of the mouse (Mus musculus L.)
38. Histone macroH2A1.2 relocates to the inactive X chromosome after initiation and propagation of X-inactivation
39. X-chromosome inactivation: Molecular mechanisms and genetic consequences
40. Experimental genetic mosaicism in the mouse
41. X inactivation: Tsix and Xist as yin and yang
42. Changes in DNA methylation during mouse embryonic development in relation to X-chromosome activity and imprinting
43. Decrease in DNA methylase activity during preimplantation development in the mouse
44. XIST RNA paints the inactive X chromosome at interphase: Evidence for a novel RNA involved in nuclear/chromosome structure
45. Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres
46. Evidence that random and imprinted Xist expression is controlled by preemptive methylation
47. Developments in quantitative PCR
48. DNA hypomethylation can activate Xist expression and silence X-linked genes
49. RNA and the epigenetic regulation of X chromosome inactivation
50. X chromosome inactivation is mediated by Xist RNA stabilization
51. Real-time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres
52. The search for the mouse X-chromosome inactivation centre
53. The use of X-autosome translocations in locating the X-chromosome inactivation center
54. Regulation of imprinted Xchromosome inactivation in mice by Tsix
55. Stabilization of Xist RNA mediates initiation of X chromosome inactivation
56. Variable X chromosome inactivation patterns in neartetraploid murine EC × somatic cell hybrid cells differentiated in vitro
57. Preferential inactivation of the paternally derived X chromosome in the extraembryonic membranes of the mouse
58. Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage
59. La teneur du noyau cellulaire en acide desoxyribonucleique a travers les organes, les individus et les especes animales
60. The differential staining pattern of the X chromosome in the embryonic and extraembryonic tissues of postimplantation homozygous tetraploid mouse embryos
61. Preimplantation differentiation in the mouse egg as revealed by microinjection of vital markers
62. A shift from reversible to irreversible X inactivation is triggered during ES cell differentiation
63. The developmental potential of mouse 16-cell blastomeres