Assay and screening methods for bioactive substances based on cellular signaling pathways

Reviews in Molecular Biotechnology

Volumn 82, Issue 4, 2002, Pages 357-370

  Umezawa, Yoshio ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Antigen specific IgE; cGMP; MRP; Protein phosphorylation; Protein protein interaction

Indexed keywords

MAMMALIA;

CYCLIC GMP; GLYCOPROTEIN P; IMMUNOGLOBULIN E; INSULIN;

ANTIGEN ANTIBODY REACTION; BIOTECHNOLOGY; CHEMISTRY; MAST CELL; METABOLISM; METHODOLOGY; PHOSPHORYLATION; PHYSIOLOGY; PROTEIN BINDING; REVIEW; SIGNAL TRANSDUCTION;

ANTIGEN-ANTIBODY REACTIONS; BIOTECHNOLOGY; CYCLIC GMP; IMMUNOGLOBULIN E; INSULIN; MAST CELLS; P-GLYCOPROTEINS; PHOSPHORYLATION; PROTEIN BINDING; SIGNAL TRANSDUCTION;

EID: 0036181260     PISSN: 13890352     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-0352(01)00051-4     Document Type: Article

References (17)

1. A screening method for antigen-specific IgE using mast cells based on intracellular calcium signaling
2. Membrane topology and glycosylation of the human multidrug resistance-associated protein
3. Evaluation of agonist selectivity for the NMDA receptor ion channel in bilayer lipid membranes based on integrated single-channel currents
4. Evaluation and comparison of ion permeation and agonist selectivities for N-methl-D-aspartate receptor channels with different submit compositions in bilayer lipid membranes based on integrated single-channel currents
5. An optical method for evaluating ion selectivity for calcium signaling pathways in the cell
6. An assay method for evaluating chemical selectivity of agonists for insulin signaling pathways based on agonist-induced phosphorylation of a target peptide
7. Metal ion selectivity for formation of the calmodulin-metal-target peptide ternary complex studied by surface plasmon resonance spectroscopy
8. 2+? Surface plasmon resonance and FT-IR spectroscopic studies
9. A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing
10. Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing
11. A screening method for substrates of multidrug resistance-associated protein (MRP)
12. A method for evaluating chemical selectivity of agonists for glutamate receptor channels incorporated in liposomes based on an agonist-induced ion flux measured by ion-selective electrodes
13. A fluorescent indicator for tyrosine phosphorylation-based insulin signaling pathways
14. Fluorescent indicators for cyclic GMP based on cyclic GMP-dependent protein kinase Iα and green fluorescent proteins
15. Structure and expression of the messenger RNA encoding the murine multidrug resistance protein, an ATP-binding cassette transporter
16. Methods of analysis
17. An SPR-based screening method for agonist selectivity for insulin signaling pathways based on the binding of phosphotyrosine to its specific binding protein