Production of a novel FK520 analog in Streptomyces hygroscopicus: Improving titer while minimizing impurities

Journal of Industrial Microbiology and Biotechnology

Volumn 28, Issue 1, 2002, Pages 12-16

  Regentin, R ^a   Cadapan, L ^a   Ou, S ^a   Zavala, S ^a   Licari, P ^a  

^a Kosan Biosciences ^*  (United States)

Author keywords

Ascomycin; Fermentation; FK520; Streptomyces hygroscopicus

Indexed keywords

ACYLTRANSFERASE; ASCOMYCIN; OXYGEN; POLYKETIDE SYNTHASE;

ARTICLE; DRUG IMPURITY; DRUG MANUFACTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; NONHUMAN; STREPTOMYCES HYGROSCOPICUS;

BACTERIOLOGICAL TECHNIQUES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CULTURE MEDIA; FERMENTATION; HYDROGEN-ION CONCENTRATION; INDUSTRIAL MICROBIOLOGY; MOLECULAR STRUCTURE; OXYGEN; STREPTOMYCES; TACROLIMUS;

STREPTOMYCES; STREPTOMYCES HYGROSCOPICUS;

EID: 0036165304     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj/jim/7000208     Document Type: Article

References (10)

1. A functional chimeric modular polyketide synthase generated via domain replacement
2. Structural modification of the immunosuppressants FK-506 and ascomycin using a biological approach
3. Pharmacokinetic study of FK506 in the rat
4. Manipulation of modular polyketide synthases
5. Metabolism of the immunosuppressant tacrolimus in the small intestine: Cytochrome P450, drug interactions, and interindividual variability
6. Improved erythromycin production in a genetically engineered industrial strain of Saccharopolyspora erythraea
7. The biosynthetic gene cluster for the macrolactone ring of the immunosuppressant FK506
8. Chemical and biological characterization of two FK506 analogs produced by targeted gene disruption in Streptomyces sp. MA6548
9. In vitro metabolism of FK506 in rat, rabbit, and human liver microsomes: Identification of a major metabolite and of cytochrome P450 3A as the major enzymes responsible for its metabolism
10. The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC14891) contains genes for biosynthesis of unusual polyketide extender units