Expression and purification of catalytic domain of human macrophage elastase for high-throughput inhibitor screening

Acta Pharmacologica Sinica

Volumn 23, Issue 2, 2002, Pages 143-151

  Cheng, Dong Hang ^a   Shen, Qiang ^a   Qian, Jing ^a   Qian, Zhen ^a   Ye, Qi Zhuang ^a  

^a Chinese Academy of Sciences   (China)

Author keywords

Gene expression; High throughput screening; Human macrophage elastase; Matrix metalloproteinases

Indexed keywords

CALCIUM ION; CHROMOGENIC SUBSTRATE; ENZYME INHIBITOR; MACROPHAGE ELASTASE; MACROPHAGE ELASTASE INHIBITOR; UNCLASSIFIED DRUG; ZINC ION; METALLOPROTEINASE; MMP12 PROTEIN, HUMAN; RECOMBINANT PROTEIN;

ANALYTIC METHOD; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; DRUG SCREENING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ESCHERICHIA COLI; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PH; PROTEIN EXPRESSION; PROTEIN FOLDING; AMINO ACID SEQUENCE; BIOSYNTHESIS; DRUG ANTAGONISM; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; MACROPHAGE; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; ESCHERICHIA COLI; HUMANS; MACROPHAGES; MATRIX METALLOPROTEINASE 12; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0036163189     PISSN: 16714083     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Structural properties of matrix metalloproteinases
2. The matrix-degrading metalloproteinases
3. Matrilysin is expressed by lipid-laden macrophages at sites of potential rapture in atherosclerotic lesions and localizes to areas of versican deposition, a proteoglycan substrate for the enzyme
4. Contributions of tumor and stromal matrix metalloproteinases to tumor progression, invasion and metastasis
5. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice
6. Cloning and characterization of a unique elastolytic metalloproteinase produced by human alveolar macrophages
7. Macrophage metalloelastase degrades matrix and myelin proteins and processes a tumour necrosis factor-alpha fusion protein
8. Purification of active matrix metalloproteinase catalytic domains and its use for screening of specific stromelysin-3 inhibitors
9. A recombinant human stromelysin catalytic domain identifying tryptophan derivatives as human stromelysin inhibitors
10. Reconstructed 19 kD a catalytic domain of gelatinase A is an active proteinase
11. Purification and characterization of the human stromelysin catalytic domain expressed in Escherichia coli
12. Recombinant human 92-kDa type IV collagenase/gelatinase from baculovirus-infected insect cells: Expression, purification, and characterization
13. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
14. High-throughput screening for human collagenase 1 inhibitors
15. Macrophage elastase (MMP-12)
16. Large-scale expression, refolding, and purification of the catalytic domain of human macrophage metalloelastase (MMP-12) in Escherichia coli
17. Mechanisms of activation of tissue procollagenase by matrix metalloproteinase 3 (stromelysin)
18. Activation mechanisms of matrix metalloproteinases
19. Hydrolysis of a broad spectrum of extracellular matrix proteins by human macrophage elastase