MALDI/MS-based epitope mapping of antigens bound to immobilized antibodies

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 20, Issue 1, 2002, Pages 49-62

  Parker, Carol E ^a   Tomer, Kenneth B ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

Epitope mapping; HIV p26; MALDI MS; Mass spectrometry; Proteolytic footprinting

Indexed keywords

CARBOXYPEPTIDASE C; EPITOPE; PROTEIN P26; RECOMBINANT PROTEIN; SEPHAROSE; UNCLASSIFIED DRUG;

ANTIGEN ANTIBODY COMPLEX; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BINDING KINETICS; CRYSTALLIZATION; DNA FOOTPRINTING; EPITOPE MAPPING; INSTRUMENTATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; PROTEIN DEGRADATION; PROTEIN PURIFICATION;

EID: 0036157245     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/mb:20:1:049     Document Type: Article

References (27)

1. Protein epitope mapping by mass spectrometry
2. Mapping protein-protein interactions by affinity-directed mass spectrometry
3. Molecular epitope identification by limited proteolysis of an immobilized antigen-antibody complex and mass spectrometric peptide mapping
4. Mass spectrometric approaches to the characterization of tertiary and supramolecular structures of biomolecules
5. Mapping epitopes on a protein antigen by the proteolysis of antigen-antibody complexes
6. Epitope mapping by chemical modification of free and antibody-bound protein antigen
7. Mass spectrometric mapping of protein epitope structures of myocardial infarct markers myoglobin and troponin T
8. Epitope-mapping of the gastrin-releasing peptide/anti-bombesin monoclonal antibody complex by proteolysis followed by matrix-assisted laser desorption mass spectrometry
9. Antigenic surveillance of the influenza virus by mass spectrometry
10. Direct identification of protein epitopes by mass spectrometry without immobilization of antibody and isolation of antibody-peptide complexes
11. IIIB p26 recognized by a monoclonal antibody
12. Matrix-assisted laser desorption ionization mass spectrometry mapping of human immunodeficiency virus-gp120 epitopes recognized by a limited polyclonal antibody
13. Characterization of a discontinuous epitope of the human immunodeficiency virus (HIV) core protein p24 by epitope excision and differential chemical modification followed by mass spectrometric peptide mapping analysis
14. Mass spectrometric characterization of a discontinuous epitope of the human immunodeficiency virus (HIV) envelope protein HIV-gp120 recognized by the human monoclonal antibody 1331A
15. Characterization of an anti-Borrelia burgdorferi OspA conformational epitope by limited proteolysis of monoclonal antibody-bound antigen and mass spectrometric peptide mapping
16. Monitoring cleavage of fusion proteins by matrix-assisted laser desorption ionization/mass spectrometry: Recombinant HIV-1IIIB p26
17. Monoclonal antibodies specific to p24, the major core protein of human T-cell leukemia virus type III
18. Biochemical and immunological analysis of human immunodeficiency virus gag gene products p17 and p24
19. Expression and purification of glutathione-S-transferase fusion proteins
20. Reactivity of an HIV gag gene polypeptide expressed in E. coli with sera from AIDS patients and monoclonal antibodies to gag
21. Epitope mapping of the HIV-1 gag region by analysis of gag gene deletion fragments expressed in Escherichia coli defines eight antigenic determinants
22. Affinity capillary electrophoresis investigation of an epitope on human immunodeficiency virus recognized by a monoclonal antibody
23. Human immunodeficiency virus type 1 gag protein binds to cyclophilins A and B
24. Crystal structure of dimeric HIV-1 capsid protein
25. Structure of the amino-terminal core domain of the HIV-1 capsid protein