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Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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15N edited HMQC-NOESY-HSQC data [L. E. Kay, G. M. Clore, A. Bax, A. M. Gronenborn, Science 249, 411 (1990); D. R. Muhandiram, G. Y. Xu, L. E. Kay, J. Biomol. NMR 3, 463 (1993)].
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15N edited HMQC-NOESY-HSQC data [L. E. Kay, G. M. Clore, A. Bax, A. M. Gronenborn, Science 249, 411 (1990); D. R. Muhandiram, G. Y. Xu, L. E. Kay, J. Biomol. NMR 3, 463 (1993)].
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15N edited HMQC-NOESY-HSQC data [L. E. Kay, G. M. Clore, A. Bax, A. M. Gronenborn, Science 249, 411 (1990); D. R. Muhandiram, G. Y. Xu, L. E. Kay, J. Biomol. NMR 3, 463 (1993)].
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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Wüthrich, K.1
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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2 and best-fit backbone root mean square (rms) deviations of 1.3 ± 0.3 Å. Backbone hydrogen bond restraints identified with the use of DIANA, and additional NOE restraints, were included in subsequent rounds of refinement, which led to improved convergence but did not alter the global fold. Color figures were generated with the Midas-plus [T. E. Ferrin, C. C. Huang, L. E. Jarvis, R. Langridge, J. Mol. Graph. 6, 13 (1988], Molscript [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)], and Raster-3D [D. J. Bacon and W. F. Anderson, J. Mol. Graph. 6, 219 (1988); E. A. Merritt and M. E. P. Murphy, Acta Crystallogr. D50, 869 (1994)] software packages.
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Supported by NIH grant AI30917 (M.F.S.), an award from the Searle Scholars Program (W.I.S.), a grant from the Lucille P. Markey Charitable Trust, and the University of Utah Cancer Center Support Grant (CA 42014). NMR instrumentation was purchased with partial support from NIH grant GM 42561. J.W. is supported by a Meyerhoff scholarship from the University of Maryland Baltimore County (UMBC). We are grateful to T. O'Hern (Howard Hughes Medical Institute, UMBC) and M. Zawrotny (UMBC) for technical support.
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