Enhanced exopolysaccharide production by metabolic engineering of Streptococcus thermophilus

Applied and Environmental Microbiology

Volumn 68, Issue 2, 2002, Pages 784-790

  Levander, Fredrik ^a   Svensson, Malin ^a   Rådström, Peter ^a  

^a LUND INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; GENES; METABOLISM; METABOLITES; POLYSACCHARIDES;

MUTANTS;

BACTERIA;

BACTERIAL POLYSACCHARIDE; GALACTOSE; GLUCOSE 1 PHOSPHATE; LACTOSE; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE;

POLYSACCHARIDE;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CARBOHYDRATE METABOLISM; CARBON SOURCE; ENZYME ACTIVITY; FERMENTATION; GALU GENE; GENE OVEREXPRESSION; METABOLIC ENGINEERING; METABOLIC REGULATION; MOLECULAR CLONING; NONHUMAN; STREPTOCOCCUS THERMOPHILUS;

BASE SEQUENCE; CLONING, MOLECULAR; CULTURE MEDIA; GALACTOSE; GENETIC ENGINEERING; MOLECULAR SEQUENCE DATA; PHOSPHOGLUCOMUTASE; POLYSACCHARIDES, BACTERIAL; STREPTOCOCCUS; UTP-GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE;

BACTERIA (MICROORGANISMS); POSIBACTERIA; PROKARYOTA; STREPTOCOCCUS; STREPTOCOCCUS THERMOPHILUS; TRIXIS;

EID: 0036156924     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.2.784-790.2002     Document Type: Article

References (38)

1. The complete cps gene cluster from Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of a new exopolysaccharide
2. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
3. Control aspects of uridine 5′-diphoshate glucose and thymidine 5′-diphosphate glucose synthesis by microbial enzymes
4. High-efficiency gene inactivation and replacement system for gram-positive bacteria
5. Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis
6. Are horizontal transfers involved in the evolution of the Streptococcus thermophilus exopolysaccharide synthesis loci?
7. Functional analysis in type Ia group B Streptococcus of a cluster of genes involved in extracellular polysaccharide production by diverse species of streptococci
8. Correlation of activities of the enzymes α-phosphoglucomutase, UDP-galactose 4-epimerase, and UDP-glucose pyrophosphorylase with exopolysaccharide biosynthesis by Streptococcus thermophilus LY03
9. Indication that the nitrogen source influences both amount and size of exopolysaccharides produced by Streptococcus thermophilus LY03 and modelling of the bacterial growth and exopolysaccharide production in a complex medium
10. UDP-N-acetylglucosamine 4-epimerase activity indicates the presence of N-acetyl-galactosamine in exopolysaccharides of Streptococcus thermophilus strains
11. Heteropolysaccharides from lactic acid bacteria
12. Lactose transport system of Streptococcus thermophilus. Functional reconstitution of the protein and characterization of the kinetic mechanism of transport
13. + ratio
14. Heterologous expression of an immunogenic pneumococcal type 3 capsular polysaccharide in Lactococcus lactis
15. A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol
16. Capsule biosynthesis and basic metabolism in Streptococcus pneumoniae are linked through the cellular phosphoglucomutase
17. Galactokinase activity in Streptococcus thermophilus
18. High efficiency transformation of Escherichia coli with plasmids
19. Requirement for phosphoglucomutase in exopolysaccharide biosynthesis in glucose- and lactose-utilizing Streptococcus thermophilus
20. Small-scale analysis of exopolysaccharides from Streptococcus thermophilus grown in a semi-defined medium
21. Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure
22. Characterization of the galU gene of Streptococcus pneumoniae encoding a uridine diphosphoglucose pyrophosphorylase: A gene essential for capsular polysaccharide biosynthesis
23. Directed genomic integration, gene replacement, and integrative gene expression in Streptococcus thermophilus
24. Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis
25. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis
26. Electrotransformation of industrial strains of Streptococcus thermophilus
27. The concentration of red blood cell UDP glucose and UDP galactose determined by high-performance liquid chromatography
28. Energy transduction in lactic acid bacteria
29. Purification and characterization of two phosphoglucomutases from Lactococcus lactis subsp. lactis and their regulation in maltose- and glucose-utilizing cells
30. Biochemistry: How to make a superior cell
31. Identification and characterization of the eps (exopolysaccharide) gene cluster from Streptococcus thermophilus Sfi6
32. Selection of galactose-fermenting Streptococcus thermophilus in lactose-limited chemostat cultures
33. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools
34. Genetics and engineering of microbial exopolysaccharides for food: Approaches for the production of existing and novel polysaccharides
35. Nutrient requirements of lactococci in defined growth media
36. Activation of silent gal genes in the lac-gal regulon of Streptococcus thermophilus
37. Biological functions of UDP-glucose synthesis in Streptococcus mutans