Transcriptional and proteomic analysis of a ferric uptake regulator (Fur) mutant of shewanella oneidensis: Possible involvement of Fur in energy metabolism, transcriptional regulation, and oxidative stress

Applied and Environmental Microbiology

Volumn 68, Issue 2, 2002, Pages 881-892

  Thompson, Dorothea K ^a   Beliaev, Alexander S ^a   Giometti, Carol S ^a   Tollaksen, Sandra L ^a   Khare, Tripti ^a   Lies, Douglas P ^a   Nealson, Kenneth H ^a   Lim, Hanjo ^a   Yates III, John ^a   Brandt, Craig C ^a   Tiedje, James M ^a   Zhou, Jizhong ^a  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; ELECTROPHORESIS; GENES; IRON; MASS SPECTROMETRY; METABOLISM; PROTEINS;

OXIDATIVE STRESSES;

MICROBIOLOGY;

CYTOCHROME B; CYTOCHROME C; FERRIC UPTAKE REGULATOR; GENE PRODUCT; REPRESSOR PROTEIN; SIDEROPHORE; UNCLASSIFIED DRUG;

BACTERIUM;

ANAEROBIC GROWTH; ARTICLE; BACTERIUM MUTANT; DNA MICROARRAY; ENERGY METABOLISM; GENE REPRESSION; IRON TRANSPORT; KNOCKOUT GENE; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEOMICS; SHEWANELLA; SHEWANELLA ONEIDENSIS; TRANSCRIPTION REGULATION;

NON-PROGRAMMATIC;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENERGY METABOLISM; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, BACTERIAL; IRON; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OXIDATIVE STRESS; PROTEOME; REPRESSOR PROTEINS; SEQUENCE ANALYSIS, DNA; SHEWANELLA; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PROKARYOTA; SHEWANELLA; SHEWANELLA ONEIDENSIS; SHEWANELLA ONEIDENSIS MR-1; TRIXIS;

EID: 0036156696     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.2.881-892.2002     Document Type: Article

References (73)

1. The pKNOCK series of broad-host-range mobilizable suicide vectors for gene knockout and targeted DNA insertion into the chromosome of gram-negative bacteria
2. Analytical techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: Multiple isoelectric focusing
3. Analytical techniques for cell fractions. XXII. Two-dimensional analysis of serum and tissue proteins: Multiple gradient slab-gel electrophoresis
4. The Tycho system for computer analysis of two-dimensional gel electrophoresis patterns
5. Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli
6. Cloning and initial characterization of the Bordetella pertussis fur gene
7. Shewanella putrefaciens mtrB encodes an outer membrane protein required for Fe(III) and Mn(IV) reduction
8. Gene and protein expression profiles of Shewanella oneidensis during anaerobic growth with different electron acceptors
9. Identification and cloning of a fur homolog from Neisseria gonorrhoeue
10. Bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated Bordetella bronchiseptica mutants
11. Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli
12. Bacillus subtilis contains multiple Fur homologues: Identification of the iron uptake (Fur) and peroxide regulon (PerR) repressors
13. Cloning, sequencing, and transcriptional regulation of viuA, the gene encoding the ferric vibriobactin receptor of Vibrio cholerae
14. Iron regulation of Shiga-like toxin expression in Escherichia coli is mediated by the fur locus
15. A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens
16. A gene cluster involved in the utilization of both free heme and heme: Hemopexin by Haemophilus influenzae type b
17. Metal iron regulation of gene expression. Fur repressor-operator interaction at the promoter region of the aerobactin system of pColV-K30
18. Fur (ferric uptake regulation) protein and CAP (catabolite-activator protein) modulate transcription of fur gene in Escherichia coli
19. Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor
20. An approach to correlate mass spectral data of peptides with amino acid sequences in a protein database
21. Effect of Salmonella typhimurium ferric uptake regulator (fur) mutations on iron- and pH-regulated protein synthesis
22. Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatography-microspray and nanospray mass spectrometry
23. Quantitation of human leukocyte proteins after silver-staining: A study with two-dimensional electrophoresis
24. The application of two-dimensional electrophoresis to mutation studies
25. Transcriptional regulation by iron of a Vibrio cholerae virulence gene and homology of the gene to the Escherichia coli Fur system
26. Identification of an iron-regulated virulence determinant in Vibrio cholerae, using Tn phoA mutagenesis
27. Mechanism for iron-regulated transcription of the Escherichia coli cir gene: Metal-dependent binding of Fur protein to the promoters
28. The htpR gene product of E. coli is a sigma factor for heat-shock promoters
29. The FNR modulon and FNR-regulated gene expression
30. Identification of a genetic locus of Haemophilus influenzae type b necessary for the binding and utilization of heme bound to human hemopexin
31. Ferrous iron transport mutants in Escherichia coli K12
32. Regulatory roles of Fnr, Fur, and Arc in expression of manganese-containing superoxide dismutase in Escherichia coli
33. An operon containing fumC and sodA encoding fumarase C and manganese superoxide dismutase is controlled by the ferric uptake regulator in Pseudomonas aeruginosa: Fur mutants produce elevated alginate levels
34. Ferric uptake regulator (Fur) mutants of Pseudomonas aeruginosa demonstrate defective siderophore-mediated iron uptake, altered aerobic growth, and decreased superoxide dismutase and catalase activities
35. A concise guide to cDNA microarray analysis
36. Interaction of Pseudomonas aeruginosa with epithelial cells: Identification of differentially regulated genes by expression microarray analysis of human cDNAs
37. Suicide plasmids containing promoterless reporter genes can simultaneously disrupt and create fusions to target genes of diverse bacteria
38. Transcriptional analysis of the Bordetella alcaligin siderophore biosynthesis operon
39. Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes
40. Yeast microarrays for genome wide parallel genetic and gene expression analysis
41. Identifying the major proteome components ofHaemophilus influenzae typestrain NCTC 8143
42. Cloning, sequencing, and transcriptional regulation of the Vibrio cholerae fur gene
43. Analysis of the complexity of gene regulation by Fur in vibrio cholerae
44. Role of iron in regulation of virulence genes
45. Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns
46. Dissimilatory metal reduction
47. Isolation and characterization of a Shewanella putrefaciens MR-1 electron transport regulator etrA mutant: Reassessment of the role of EtrA
48. Ferric reductase is associated with the membranes of anaerobically grown Shewanella putrefaciens MR-1
49. Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor
50. Iron and manganese in anaerobic respiration: Environmental significance, physiology, and regulation
51. Control of Escherichia coli superoxide dismutase (sodA and sodB) genes by the ferric uptake regulation (fur) locus
52. Exotoxin A production in Pseudomonas aeruginosa requires the ironregulated alternative sigma factor PvdS
53. Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: Cycle selection of iron-regulated genes
54. High resolution two-dimensional electrophoresis of proteins
55. Purification and biochemical characterization of a putative [6Fe-6S] prismane-cluster-containing protein from Desulfovibrio vulgaris (Hildenborough)
56. Coordinate regulation of siderophore and exotoxin A production: Molecular cloning and sequencing of the Pseudomonas aeruginosa fur gene
57. Quantitation of microgram amounts of protein in two-dimensional polyacrylamide gel electrophoresis sample buffer
58. Sequence and genetic characterization of etrA, an fur analog that regulates anaerobic respiration in Shewanella putrefaciens MR-1
59. Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes
60. Universal chemical assay for the detection and determination of siderophores
61. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels
62. Pleiotropic effects of a Yersinia pestis fur mutation
63. The primary structure of a protein containing a putative [6Fe-6S] prismane cluster from Desulfovibrio desulfuricans (ATCC 27774)
64. Wholegenome expression analysis of snf/swi mutants of Saccharomyces cerevisiae
65. Identification and cloning of a fur homolog from Neisseria meningitidis
66. Isolation and analysis of a fur mutant of Neisseria gonorrhoeae
67. Characterization of the Vibrio anguillarum fur gene: Role in regulation of expression of the FatA outer membrane protein and catechols
68. Iron regulation of siderophore biosynthesis and transport in Pseudomonas putida WCS358: Involvement of a transcriptional activator and of the Fur protein
69. Phosphorus assimilation and control of the phosphate regulon
70. The interaction of the Vibrio cholerae transcription factors, Fur and IrgB, with the overlapping promoters of two virulence genes, irgA and irgB
71. Molecular characterization of the ferric-uptake regulator, fur, from Staphylococcus aureus
72. Global gene expression profiles of Bacillus subtilis grown under anaerobic conditions